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In order to explore new probiotic sources, this study investigated the potential of fructose-loving lactic acid bacteria (FLAB) from bee wax. From 15 isolated bacterial strains, AG1.4 strain was identified as the most promising probiotic strain. AG1.4 was classified under the Lactiplantibacillus group with 100% similarity. This strain demonstrated a high adhesion ability to solvents, reaching 91.65%, and exhibited strong antibacterial activity against E. coli and S. aureus, with inhibition zones measuring 12.67 mm and 14.33 mm, respectively. Additionally, AG1.4 showed resistance to all three antibiotics: ampicillin (10 µg/mL), tetracycline (30 µg/mL), and ofloxacin (10 µg/mL), and it was the only strain capable of simultaneously degrading starch, protein, and cellulose. The ability to utilize fructose and the unique biological properties provided by strain AG1.4 open new avenues for the development of novel functional fermented products, diversifying probiotic sources, ensuring food safety, and enhancing the nutritional value for humans.