Phân lập và tuyển chọn nấm men chịu nhiệt và lên men bốn loại dịch ép trái cây
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Abstract
The selection of heat-tolerant yeasts capable of fermenting a wide variety of fruits is crucial in the beverage fermentation industry. This approach not only helps reduce cooling costs during fermentation but also enables the use of diverse raw materials. The research results isolated and selected 6 yeast strains capable of producing ethanol at concentrations of 4-10%. Of these, 5 strains grew at 45oC in YPD broth medium. The MR strain isolated from jackfruit has with following characteristics: able to grow at 45oC; pH suitable at 3.5 and adaptability on ethanol fermentation with guava, starfruit, jackfruit and oranges with ethanol concentrations of 10%, 10%, 8% and 6% respectively. Sequence analysis of the MR strain’s 18S rRNA gene revealed 91.34% similarity with Saccharomyces cerevisiae AUMC 10233. Further research and investigation of optimal conditions, including temperature, pH, fermentation time and fruit type, is needed to maximize the alcoholic fermentation potential of the MR strain, in order to improve ethanol production efficiency and wine quality on a variety of fruits.
Tóm tắt
Việc tuyển chọn nấm men chịu nhiệt và lên men nhiều loại trái cây là rất cần thiết trong lĩnh vực lên men đồ uống, giúp giảm chi phí làm mát và áp dụng trên nhiều nguyên liệu. Trong nghiên cứu, 6 dòng nấm men có khả năng sản xuất ethanol với nồng độ từ 4 đến10% đã được phân lập và tuyển chọn. Trong đó, 5 dòng phát triển ở nhiệt độ 45oC trong môi trường YPD lỏng. Qua khảo sát sàng lọc, dòng MR phân lập từ mít, có thể phát triển ở nhiệt độ 45oC, pH 3,5 và lên men được các loại trái cây như ổi, khế, mít và cam sành đạt nồng độ ethanol tương ứng 10%, 10%, 8% và 6% được chọn. Trình tự gen 18S rRNA của dòng MR được phân tích cho thấy mức độ tương đồng 91,34% với Saccharomyces cerevisiae AUMC 10233. Các điều kiện nhiệt độ, pH, thời gian lên men và từng loại trái cây được tiếp tục khảo sát để khai thác tối đa khả năng lên men của dòng MR, nhằm nâng cao hiệu suất sản xuất ethanol và đa dạng nguyên liệu.
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