Đặng Hoàng Đạo * Nguyễn Thị Thu Trang

* Tác giả liên hệ (dh.dao@hutech.edu.vn)

Abstract

This study aimed to determine the prevalence of subclinical ketosis (SCK), subacute ruminal acidosis (SARA), and their co-occurrence in Holstein Friesian dairy cows at Vinamilk Dairy Farm, Da Lat during the early postpartum period. Rumen fluid and blood samples were collected from 200 cows to measure pH and β-hydroxybutyrate (BHB) concentrations. The results showed that the prevalence of SCK was 22%, SARA was 1%, and the co-occurrence rate was 2.5%. Correlation analysis revealed a significant inverse correlation between BHB and rumen pH (r = -0.16; P = 0.02), and a positive correlation between days in milk (DIM) and BHB (r = 0.25; P = 0.0004). Body condition score loss (BCS loss) tended to increase the risk of SCK and SARA. The study concluded that SCK and SARA can co-occur due to negative energy balance, and simultaneous monitoring of BHB, rumen pH, and BCS loss is necessary for early detection of metabolic disorders in high-yielding dairy cows.

Keywords: β-hydroxybutyrate, body condition score, Holstein Friesian dairy cows, negative energy balance, subacute ruminal acidosis, subclinical ketosis

Tóm tắt

Nghiên cứu này được thực hiện nhằm xác định tỷ lệ mắc ketosis cận lâm sàng (SCK), acidosis dạ cỏ bán cấp tính (SARA) và tỷ lệ đồng mắc giữa hai bệnh này ở bò sữa Holstein Friesian tại trại Vinamilk Đà Lạt trong giai đoạn đầu sau đẻ. Mẫu dịch dạ cỏ và máu được thu thập từ 200 con bò để đo pH và nồng độ β-hydroxybutyrate (BHB). Kết quả cho thấy tỷ lệ SCK là 22%, SARA là 1% và tỷ lệ đồng mắc là 2,5%. Phân tích tương quan cho thấy mối tương quan nghịch có ý nghĩa giữa BHB và pH dạ cỏ (r = -0,16; P = 0,02) và DIM có tương quan thuận với BHB (r = 0,25; P = 0,0004). Mức giảm điểm thể trạng (BCS loss) có xu hướng làm tăng nguy cơ SCK và SARA. Nghiên cứu kết luận rằng SCK và SARA có thể đồng phát do mất cân bằng năng lượng âm và việc giám sát đồng thời BHB, pH dạ cỏ và BCS loss là cần thiết để phát hiện sớm rối loạn chuyển hóa ở bò sữa.

Từ khóa: Acidosis dạ cỏ bán cấp tính, β-hydroxybutyrate, bò sữa Holstein Friesian, ketosis cận lâm sàng, cân bằng năng lượng âm, điểm thể trạng

Article Details

Tài liệu tham khảo

Antanaitis, R., Juozaitienė, V., Malašauskienė, D., & Televičius, M. (2019). Can rumination time and some blood biochemical parameters be used as biomarkers for the diagnosis of subclinical acidosis and subclinical ketosis? Veterinary and Animal Science, 8, 100077. https://doi.org/10,1016/j.vas.2019,100077

Balan, C. (2025). Prevalence of ketosis in Indian dairy bovines: A meta-analysis. International Journal of Veterinary Sciences and Animal Husbandry, 10(8), 161–165.

Breda, J. C. dos S., Facury Filho, E. J., Flaiban, K. K. da C., & Lisboa, J. A. N. (2023). Effect of Parity, Body Condition Score at Calving, and Milk Yield on the Metabolic Profile of Gyr Cows in the Transition Period. Animals, 13(15), 2509. https://doi.org/10,3390/ani13152509

Brunner, N., Groeger, S., Canelas Raposo, J., Bruckmaier, R. M., & Gross, J. J. (2019). Prevalence of subclinical ketosis and production diseases in dairy cows in Central and South America, Africa, Asia, Australia, New Zealand, and Eastern Europe. Translational Animal Science, 3(1), 84–92.
https://doi.org/10,1093/tas/txy102

Cascone, G., Licitra, F., Stamilla, A., Amore, S., Dipasquale, M., Salonia, R., Antoci, F., & Zecconi, A. (2022). Subclinical Ketosis in Dairy Herds: Impact of Early Diagnosis and Treatment. Frontiers in Veterinary Science, 9, 895468. https://doi.org/10,3389/fvets.2022,895468

Castillo-Lopez, E., Hartinger, T., Farghaly, M. M., Reisinger, N., Lang, C., Klambauer, L., Huber, J., & Zebeli, Q. (2025). Differences in severity of reticulo-rumen pH drop in primiparous Holstein cows fed the same diet during transition and early lactation: Effects on performance, energy balance, blood metabolites, and reproduction. Journal of Animal Science, 103, skae390. https://doi.org/10,1093/jas/skae390

Chapinal, N., Carson, M. E., LeBlanc, S. J., Leslie, K. E., Godden, S., Capel, M., Santos, J. E. P., Overton, M. W., & Duffield, T. F. (2012). The association of serum metabolites in the transition period with milk production and early-lactation reproductive performance. Journal of Dairy Science, 95(3), 1301–1309. https://doi.org/10,3168/jds.2011-4724

de Assis Lage, C. F., Räisänen, S. E., Melgar, A., Nedelkov, K., Chen, X., Oh, J., Fetter, M. E., Indugu, N., Bender, J. S., Vecchiarelli, B., Hennessy, M. L., Pitta, D., & Hristov, A. N. (2020). Comparison of two sampling techniques for evaluating ruminal fermentation and microbiota in the planktonic phase of rumen digesta in dairy cows. Frontiers in Microbiology, 11, 618032. https://doi.org/10.3389/fmicb.2020.618032

Duffield, T. F. (2000). Subclinical ketosis in lactating dairy cattle. Veterinary Clinics of North America: Food Ani. Pra., 16(2): 231-53. https://doi.org/10.1016/S0749-0720(15)30103-1

Garrett, E. F., Pereira, M. N., Nordlund, K. V., Armentano, L. E., Goodger, W. J., & Oetzel, G. R. (1999). Diagnostic Methods for the Detection of Subacute Ruminal Acidosis in Dairy Cows. Journal of Dairy Science, 82(6), 1170–1178. https://doi.org/10,3168/jds.S0022-0302(99)75340-3

Geishauser, T., Linhart, N., Neidl, A., & Reimann, A. (2012). Factors associated with ruminal pH at herd level. Journal of Dairy Science, 95(8), 4556–4567.
https://doi.org/10,3168/jds.2012-5380

Khafipour, E., Krause, D. O., & Plaizier, J. C. (2009). A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation. Journal of Dairy Science, 92(3), 1060–1070. https://doi.org/10,3168/jds.2008-1389

LeBlanc, S. (2010). Monitoring metabolic health of dairy cattle in the transition period. The Journal of Reproduction and Development, 56 Suppl, S29-35.
https://doi.org/10,1262/jrd.1056s29

Lwanga, S. K., Lemeshow, S., & Organization W. H. (1991). Sample size determination in health studies: A practical manual. World Health Organization. https://iris.who.int/handle/10665/40062

McArt, J. A. A., Nydam, D. V., & Oetzel, G. R. (2012). Epidemiology of subclinical ketosis in early lactation dairy cattle. Journal of Dairy Science, 95(9), 5056–5066. https://doi.org/10,3168/jds.2012-5443

Mezzetti, M., Minuti, A., Piccioli-Cappelli, F., Amadori, M., Bionaz, M., & Trevisi, E. (2019). The role of altered immune function during the dry period in promoting the development of subclinical ketosis in early lactation. Journal of Dairy Science, 102(10), 9241–9258. https://doi.org/10,3168/jds.2019-16497

Ospina, P. A., Nydam, D. V., Stokol, T., & Overton, T. R. (2010). Evaluation of nonesterified fatty acids and β-hydroxybutyrate in transition dairy cattle in the northeastern United States: Critical thresholds for prediction of clinical diseases. Journal of Dairy Science, 93(2), 546–554. https://doi.org/10,3168/jds.2009-2277

Overton, T. R., & Waldron, M. R. (2004). Nutritional Management of Transition Dairy Cows: Strategies to Optimize Metabolic Health. Journal of Dairy Science, 87, E105–E119. https://doi.org/10,3168/jds.S0022-0302(04)70066-1

Peterson, C. K., Bolton, J., & Humphreys, B. K. (2012). Predictors of Improvement in Patients With Acute and Chronic Low Back Pain Undergoing Chiropractic Treatment. Journal of Manipulative & Physiological Therapeutics, 35(7), 525–533. https://doi.org/10,1016/j.jmpt.2012,06,003

Pinedo, P., Santos, J. E. P., Chebel, R. C., Galvão, K. N., Schuenemann, G. M., Bicalho, R. C., Gilbert, R. O., Rodriguez-Zas, S. L., Seabury, C. M., Rosa, G., & Thatcher, W. (2020). Associations of reproductive indices with fertility outcomes, milk yield, and survival in Holstein cows. Journal of Dairy Science, 103(7), 6647–6660. https://doi.org/10,3168/jds.2019-17867

Plaizier, J. C., Krause, D. O., Gozho, G. N., & McBride, B. W. (2008). Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences. The Veterinary Journal, 176(1), 21–31. https://doi.org/10,1016/j.tvjl.2007,12,016

Roche, J. R., Friggens, N. C., Kay, J. K., Fisher, M. W., Stafford, K. J., & Berry, D. P. (2009). Invited review: Body condition score and its association with dairy cow productivity, health, and welfare. Journal of Dairy Science, 92(12), 5769–5801.
https://doi.org/10,3168/jds.2009-2431

Samiei, A. (2015). Relationship between Dietary Energy Level, Silage Butyric Acid and Body Condition Score with Subclinical Ketosis Incidence in Dairy Cows. Advances in Animal and Veterinary Sciences, 3(6), 354–361. https://doi.org/10,14737/journal.aavs/2015/3,6.354,361

Vallejo, Timarán. D., Reyes-Vélez, J., VanLeeuwen, J., Maldonado-Estrada, J., & Astaiza-Martínez, J. (2020). Incidence and effects of subacute ruminal acidosis and subclinical ketosis with respect to postpartum anestrus in grazing dairy cows. Heliyon, 6(4), e03712. https://doi.org/10,1016/j.heliyon.2020.e03712

Wang, Q., Cui, Y., Indugu, N., Loor, J. J., Jiang, Q., Yu, Z., Baker, L., Pitta, D., Deng, Z., & Xu, C. (2023). Integrated meta-omics analyses reveal a role of ruminal microorganisms in ketone body accumulation and ketosis in lactating dairy cows. Journal of Dairy Science, 106(7), 4906–4917.
https://doi.org/10,3168/jds.2022-22282

Wang, Y. P., Cai, M., Hua, D. K., Zhang, F., Jiang, L. S., Zhao, Y. G., Wang, H., Nan, X. M., & Xiong, B. H. (2020). Metabolomics reveals effects of rumen-protected glucose on metabolism of dairy cows in early lactation. Animal Feed Science and Technology, 269, 114620. https://doi.org/10,1016/j.anifeedsci.2020,114620