Khảo sát hoạt tính kháng oxy hóa, kháng viêm và kháng khuẩn của cây cải trời (Blumea lacera)
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Abstract
The purpose of this study is to quantify the chemical composition and biological activity of extracts from the aerial parts (stem and leaf), stem extract, and leaf extract of Blumea lacera. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, total antioxidant capacity, ferric reducing-antioxidant power and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid). Anti-inflammatory activity was measured by its ability to protect red blood cell membranes and inhibit denaturation of bovine serum albumin. The antibacterial activity was assessed by measuring the antibacterial ring diameter, the minimum inhibitory concentration, and the minimum bactericidal concentration. Blumea lacera leaf extract exhibits stronger antioxidant and anti-inflammatory activities than the other extracts. Blumea lacera extracts inhibit Gram-positive bacteria more effectively than Gram-negative bacteria, with minimum bactericidal doses of 250 to 2000 µg/mL. Blumea lacera extracts have antioxidant, anti-inflammatory, and antibacterial properties due to the presence of polyphenols, flavonoids, and alkaloids. These findings demonstrated the potential of Blumea lacera extracts as natural antioxidants, anti-inflammatory, and antibacterial agents.
Tóm tắt
Mục đích của nghiên cứu này là định tính thành phần hóa học và hoạt tính sinh học của cao từ phần trên mặt đất (thân và lá), cao thân, cao lá cải trời (CT). Hoạt tính kháng oxy hóa (KOH) được đánh giá bằng phương pháp 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, kháng oxy hóa tổng số và tiềm năng khử và 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid). Hoạt tính kháng viêm (KV) được đo bằng khả năng bảo vệ màng tế bào hồng cầu và ức chế sự biến tính của albumin huyết thanh bò. Hoạt tính kháng khuẩn (KK) được đánh giá bằng cách đo đường kính vòng kháng khuẩn, nồng độ ức chế tối thiểu và nồng độ diệt khuẩn tối thiểu. Cao lá CT thể hiện hoạt động KOH và KV mạnh hơn các cao chiết còn lại. Các cao CT ức chế vi khuẩn Gram dương hiệu quả hơn vi khuẩn Gram âm, với liều diệt khuẩn tối thiểu từ 250 đến 2000 µg/mL. Các cao CT có đặc tính KOH, KV, KK do sự hiện diện của polyphenol, flavonoid và alkaloid. Những phát hiện này đã chứng minh tiềm năng của CT như chất KOH, KV, KK tự nhiên.
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