Huỳnh Thị Phương Thảo , Hà Thanh Toàn , Trần Chí Nhân , Nguyễn Thị Như Ý Lưu Thái Danh *

* Tác giả liên hệ (ltdanh@ctu.edu.vn)

Abstract

The study compared the antibacterial effectiveness of extracts from three green-skinned orange varieties (Sanh, Xoan and Mat oranges) and one yellow-skinned orange variety (Navel orange) by soaking and Soxhlet. The antibacterial activity of the extract was evaluated on Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. In both extraction methods and five microbial strains surveyed, extracts from green-skinned orange varieties have better antibacterial activity than yellow-skinned orange varieties, in which Xoan orange extract has the strongest antibacterial activity. The extract extracted by soaking has higher antimicrobial activity than th at extracted by Soxhlet. In addition, the extracts showed more effective antibacterial properties against Gram-positive bacteria (B. cereus, S. aureus) and fungi (C. albicans) than Gram-negative bacteria (P. aeruginosa, P. aeruginosa, E coli). In summary, the extract of Xoan orange by soaking has the strongest antibacterial properties, especially effective against S. aureus and C. albicans.

Keywords: Extract, yellow orange, green orange, antimicrobial activity, orange peel

Tóm tắt

Nghiên cứu được thực hiện nhằm so sánh hiệu quả kháng vi sinh của cao chiết từ ba giống cam vỏ xanh (cam Sành, Xoàn và Mật) và một giống cam vỏ vàng (cam Navel) bằng ngâm dầm và Soxhlet. Hoạt tính kháng vi sinh của cao chiết được đánh giá trên vi khuẩn Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli và nấm Candida albicans. Ở cả hai phương pháp trích ly và 5 chủng vi sinh được khảo sát, cao chiết từ các giống cam vỏ xanh có hoạt tính kháng vi sinh tốt hơn so với giống cam vỏ vàng, trong đó cao chiết cam Xoàn có hoạt tính kháng vi sinh mạnh nhất. Cao chiết bằng ngâm dầm có hoạt tính khánh sinh cao hơn so với cao chiết bằng Soxhlet. Ngoài ra, các loại cao chiết thể hiện khả năng kháng vi sinh hiệu quả hơn đối với vi khuẩn Gram dương (B. cereus, S. aureus) và nấm (C. albicans) so với vi khuẩn Gram âm (P. aeruginosa, E. coli). Tóm lại, cao chiết cam Xoàn bằng ngâm dầm kháng vi sinh mạnh nhất, đặc biệt hiệu quả đối với S. aureusC. albicans.

Từ khóa: Cao chiết, cam vàng, cam xanh, hoạt tính kháng vi sinh, vỏ cam

Article Details

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