Sản xuất sinh khối nấm men Saccharomyces Cerevisiae từ dịch thủy phân rong Ulva Lactuca và thử nghiệm dùng trong nuôi lưu hàu thái bình dương thương phẩm
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Abstract
Ulva lactuca seaweed, harvested in Ninh Thuan, Vietnam, underwent heat treatment at 150°C for 10 minutes, followed by enzymatic hydrolysis using Celluclast® 1.5l Novozyme at 50°C for 36 hours, resulting in a release of reducing sugars equivalent to 19.76 ± 0.27 (%) of the seaweed powder's dry weight. Utilizing the resultant seaweed hydrolysate, a yeast biomass production medium was supplemented with 9% (w/v) molasses (70oBrix) and adjusted to pH 6. A 10% (v/w) inoculum of Saccharomyces cerevisiae strain at a density of 1.2´106 cfu/ml was then introduced to the medium and fermented at room temperature for 72 hours with agitation at 120 rpm, yielding the highest yeast growth rate and wet yeast biomass production of 16.61 ± 0.95 (g/L). In a depuration test spanning 4 days, adult oysters cultured with yeast biomass derived from Ulva lactuca seaweed hydrolysate fermentation exhibited a remarkable survival rate of 93.43 ± 1.46 (%). They achieved the highest meat weight ratio of 26.45 ± 0.42 (%). This underscores the potential of Saccharomyces cerevisiae biomass cultured in Ulva lactuca hydrolysate as a viable food source for commercial Pacific oysters during the depuration process.
Tóm tắt
Rong lục Ulva lactuca thu nhận tại Ninh Thuận, Việt Nam qua xử lý nhiệt 150oC trong 10 phút và thủy phân bằng enzyme Celluclast® 1.5l Novozyme ở 50oC trong 36 giờ đã giải phóng được lượng đường khử là 19,76 ± 0,27% khối lượng khô của bột rong. Với cơ chất ban đầu là dịch rong thủy phân, môi trường sản xuất sinh khối nấm men được nghiên cứu bổ sung 9% (w/V) rỉ đường 70oBrix, điều chỉnh về pH 6. Cấy 10% (v/w) giống nấm men Saccharomyces cerevisiae mật độ 1,2x106cfu/ml vào môi trường và lên men ở nhiệt độ phòng trong 72h, tốc độ khuấy 120 vòng/ phút cho tốc độ sinh trưởng nấm men lớn nhất, lượng sinh khối nấm men ướt thu được cao nhất là 16,61±0,95 g/L. Thử nghiệm nuôi hàu trưởng thành 4 ngày bằng sinh khối nấm men trong rong Ulva lectuca thủy phân lên men cho tỷ lệ sống 93,43±1,46% và có tỷ lệ khối lượng thịt cao nhất, 26,45±0,42%. Như vậy, nấm men Saccharomyces cerevisiae trong rong lục Ulva lactuca lên men thực sự là nguồn thức ăn tiềm năng cho đối tượng hàu trưởng thành.
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