Determination of the degradability towards pretilachlor and fenclorim in herbicide by Pseudomonas sp. Pre2 and Acinetobacter sp. F0 immobilized in alginate
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Abstract
A mixed culture of two bacterial strains, Pseudomonas sp. Pre2 (a pretilachlor-degrading bacterial strain) and Acinetobacter sp. F0 (a fenclorim-degrading bacterial strain) was investigated for their degradation abilities in a mineral medium, water collected from a rice field, and a saline canal. The degradation of pretilachlor and fenclorim added in water collected from the rice field by non-immobilized bacteria was 61.9±4.8% and 50.9±6.6% after 8 hours, respectively, while the degradation by immobilized bacteria was 89.9±2.2% and 80.6±4.4%, respectively. The degradation was also carried out in a packed bed reactor to enhance the efficiency of the bacteria. In addition, the dried alginate beads, which were stored for three months, still degraded both active ingredients effectively.
Tóm tắt
Hỗn hợp hai chủng vi khuẩn là Pseudomonas sp. Pre2 (phân hủy pretilachlor) và Acinetobacter sp. F0 (phân hủy fenclorim) được khảo sát về hiệu quả phân hủy của chúng trong môi trường khoáng lỏng, nước thu về từ ruộng lúa và kênh rạch nhiễm mặn. Sự phân hủy pretilachlor và fenclorim bổ sung vào nước thu về từ ruộng lúa bởi vi khuẩn không cố định tương ứng là 61,9±4,8% và 50,9±6,6% sau 8 giờ, trong lúc sự phân hủy bởi vi khuẩn cố định tương ứng là 89,9±2,2% và 80,6±4,4%. Sự phân hủy còn được thực hiện trong hệ phân hủy đệm, giúp tăng cường hiệu quả của vi khuẩn. Ngoài ra, alginate được hút khô và bảo quản sau ba tháng vẫn có thể sử dụng hiệu quả để phân hủy cả hai hoạt chất.
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