The survey of bacterial pathogens on hemorrhagic intestinal diseased snakehead fish (Channa striata) raised commercially in Dong Thap
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Abstract
This study was to determine the pathogen of bacteria that causes hemorrhagic intestinal disease in snakehead fish (C. striata) and to provide information for effective diagnosis, prevention, and treatment of the disease. A total of 250 samples were collected in Dong Thap province, from December, 2022 to May, 2023. There were 200 diseased fish samples and 50 healthy fish samples, in which diseased fish samples had an average weight of about 226.5g and an average length of about 22.8cm. Snakehead fish with the intestine hemorrhagic disease had typically pathological signs of pale color, scattered spot hemorrhage on the body skin; the intestine were hemorrhagic, which had turned pink or red. The results of isolation, identification of bacteria and experimental infections showed that the causative agent of intestine hemorrhagic disease in snakehead fish was Aeromonas veronii. The experimental infections recorded that the median lethal dosage (LD50) of the two bacterial strains AV9 and AV39 were 4.82×105 CFU/mL and 8.36×105 CFU/mL, respectively.
Tóm tắt
Nghiên cứu được thực hiện nhằm xác định tác nhân vi khuẩn gây bệnh xuất huyết đường ruột trên cá lóc (Channa striata), từ đó cung cấp những thông tin cho việc chẩn đoán, phòng và điều trị bệnh hiệu quả. Tổng cộng 250 mẫu cá lóc được thu với 200 mẫu cá bệnh và 50 mẫu cá khỏe ở Đồng Tháp từ tháng 12/2022 đến tháng 5/2023, có trọng lượng khoảng 226,5 g và chiều dài khoảng 22,8 cm. Cá lóc bệnh xuất huyết đường ruột có dấu hiệu bệnh lý đặc trưng như màu sắc nhợt nhạt, xuất huyết dạng điểm rải rác; ruột bị xuất huyết, chuyển thành màu hồng đến đỏ. Kết quả phân lập, định danh vi khuẩn và thí nghiệm cảm nhiễm gây bệnh cho thấy, cá lóc bị bệnh xuất huyết đường ruột là do loài vi khuẩn Aeromonas veronii gây ra với giá trị độc lực LD50 của 2 chủng vi khuẩn AV9 và AV39 lần lượt là 4,82×105 CFU/mL và 8,36×105 CFU/mL.
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