Study on grafting collagen of catfish skin on hydroxyapatite from catfish bone
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Abstract
The study was conducted to graft collagen extracted from catfish skin (Pangasiidae) onto surface of hydroxyapatite (HA) synthesized from catfish bone in order to increase its applications in biomedical field. After extracting from catfish skin, collagen was grafted onto the HA surface by using glutaraldehyde as a bridge. HA particles were firstly grafted to 3 - amino propyl triethoxysilane (APTES) to form amine functional groups on the surface. Glutaraldehyde was then used as bonding bridge to graft HA and collagen through the reaction between amine and aldehyde. Factors affecting the grafting efficiency such as collagen concentration, time and temperature of the reaction were investigated. Experimental results showed that solution of 1 mg/mL collagen, reaction time of 3 hours and reaction temperature of 37°C are suitable conditions for grafting collagen onto surface of HA. In addition, the SEM images showed that size of HA particles was approximately 1,000 nm and covered by a layer of collagen after grafting.
Tóm tắt
Nghiên cứu được tiến hành nhằm gắn kết collagen trích ly từ da cá tra (Pangasiidae) lên bề mặt hydroxyapatite (HA) tổng hợp từ xương cá tra làm tăng khả năng ứng dụng trong lĩnh vực y sinh. Sau khi trích ly, collagen được gắn kết lên bề mặt HA thông qua cầu nối glutaraldehyde. Các hạt HA trước tiên gắn kết với 3 – amino propyl triethoxysilane (APTES) tạo nhóm chức amine trên bề mặt. Glutaraldehyde là cầu nối gắn kết HA và collagen thông qua phản ứng giữa nhóm chức amine và aldehyde. Các yếu tố ảnh đến sự gắn kết như nồng độ collagen, pH dung dịch phản ứng, thời gian và nhiệt độ của phản ứng được tiến hành khảo sát. Kết quả thí nghiệm cho thấy nồng độ collagen 1 mg/mL, dung dịch acetic acid hòa tan collagen có pH 3, thời gian phản ứng 3 giờ và nhiệt độ phản ứng 37C là điều kiện thích hợp để tiến hành gắn kết. Ngoài ra, kết quả chụp SEM cho thấy rằng các hạt HA được chế tạo có kích thước khoảng 1.000 nm và bị phủ một lớp collagen sau khi gắn kết.
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