Lê Vĩnh Thúc * , Parameswari Namasivayam , Huỳnh Kỳ Nguyễn Phước Đằng

* Tác giả liên hệ (lvthuc@ctu.edu.vn)

Abstract

In this study, the molecular characterization of clone Eg707 isolated from cell suspension culture of the oil palm was reported. Southern analysis result showed that Eg707 might be present as a single copy gene in the oil palm genome. This gene is highly expressed in tissue cultured materials compared to vegetative and reproductive tissues, suggesting a role of this gene during oil palm somatic embryogenesis or at the early stages of embryo development. Expression analysis of Eg707 by RNA in situ hybridization showed that Eg707 transcripts were present throughout somatic embryo development starting from proembryo formation at the embryogenic callus stages till the maturing embryo stages. Since proembryo formation within the embryogenic callus is one of the first key factors in oil palm somatic embryo development, it is suggested that Eg707 could be used as a reliable molecular marker for detecting early-stage of oil palm somatic embryogenesis.
Keywords: novel transcript, Eg707, molecular marker, somatic embryogenesis

Tóm tắt

Trong nghiên cứu này, gen Eg707 được phân lập từ tế bào nuôi cấy treo đã được khảo sát đặc tính phân tử. Kết quả phân tích Southern cho thấy Eg707 có thể là đơn gen trong bộ gen của cây cọ dầu. Gen Eg707 biểu hiện gen rất cao ở tế bào của mô được nuôi cấy hơn là ở những tế bào sinh dưỡng, điều đó chứng tỏ Eg707 có thể đóng góp trong quá trình hình thành phôi vô tính hay là trong giai đoạn đầu của quá trình phát triển phôi. Kỹ thuật lai RNA in situ một lần nữa chứng minh gen Eg707 được biểu hiện trong suốt giai đoạn phát triển phôi, sự biểu hiện gen bắt đầu từ sự hình thành phôi, từ giai đoạn callus đến giai đoạn phôi trưởng thành. Bởi vì sự hình thành mầm phôi trong thời kỳ callus phát sinh phôi là một trong những nhân tố chính cho sự phát triển phôi vô tính của cây cọ dầu, nên Eg707 có thể được đề nghị như là một đánh dấu phân tử để kiểm tra sự hình thành phôi vô tính ở giai đoạn đầu của cây cọ dầu.
Từ khóa: cọ dầu, phiên bản mới, Eg707, đánh dấu phân tử, phôi vô tính

Article Details

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