Isolation of lipid degrading bacteria from shrimp and catfish processing wastewater
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Abstract
Seafood processing is an industry contributing to food supply for local consumption, domestic markets, and export. However, wastewater from seafood processing contains a high amount of organic compounds such as lipids, which should be properly treated to avoid environmental pollution. From wastewater samples collected at a shrimp and catfish processing factory, 19 lipid-degrading bacterial isolates were obtained, of which LF2, LF4, LS3, and LS4 exhibited the highest lipase activity at 48 hours of cultivation in minimal medium supplemented with 1% sunflower oil. The isolate LS4 showed the highest lipase activity under neutral pH conditions (47.19 U/mL). Sequence analysis of the 16S-rRNA gene and biochemical characterization of LS4 confirmed that the isolate belongs to Aeromonas hydrophila and was designated as Aeromonas hydrophila LS4.
Tóm tắt
Chế biến thủy sản là ngành công nghiệp góp phần đảm bảo nguồn thực phẩm cho địa phương, trong nước và xuất khẩu. Tuy nhiên, nước thải chế biến thuỷ sản chứa nhiều chất hữu cơ, trong đó có lipid, cần được xử lý đúng cách để không gây ô nhiễm môi trường. Từ các mẫu nước thải chế biến tôm và cá da trơn, 19 dòng vi khuẩn có khả năng phân giải lipid đã được phân lập, trong đó, 4 dòng LF2, LF4, LS3 và LS4 có hoạt tính lipase cao nhất ở thời điểm 48 giờ nuôi cấy trong môi trường khoáng tối thiểu có bổ sung 1% dầu hướng dương. Dòng vi khuẩn LS4 có hoạt tính lipase cao nhất trong môi trường pH trung tính, đạt 47,19 U/mL. Kết quả so sánh trình tự gen 16S-rRNA và đặc điểm sinh hóa chứng tỏ dòng vi khuẩn LS4 thuộc loài Aeromonas hydrophila nên được định danh là Aeromonas hydrophila LS4.
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