Development in Nepenthes mirabilis extract loaded PLGA microparticles and evaluation of antioxidative stress activity
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Abstract
PLGA microparticle system loading Nepenthes mirabilis extract (NM/PLGA-NPs) was successfully developed, exhibiting encapsulation efficiency of 12.37%, 70.73% for drug loading, with average particle size of 926 nm. FT-IR spectra and SEM analyses confirmed that the encapsulation process preserved key functional groups and yielded uniform spherical morphology. The system exhibited pH-responsive drug release: rapid release at pH 1.2 and sustained release at pH 6.8 and 7.4, aligning with gastrointestinal transit. Moreover, NM/PLGA-NPs demonstrated prolonged (72-hour) DPPH radical scavenging, indicating a sustained antioxidant effect. Furthermore, cytotoxicity assays on RAW264.7 macrophages revealed biocompatibility and protective cells against H2O2-induced oxidative stress. These findings highlight the therapeutic promise of NM/PLGA-NPs in managing oxidative stress-related disorders.
Tóm tắt
Trong nghiên cứu này, hệ vi hạt PLGA chứa cao chiết từ nắp ấm hoa đôi (Nepenthes mirabilis) (NM/PLGA-NPs) đã được phát triển thành công, với hiệu suất nạp 12,37%, tải 70,73% và kích thước trung bình 926 nm. Kết quả phân tích FT-IR và SEM cho thấy quá trình nạp không làm thay đổi cấu trúc chức năng và duy trì hình thái hình cầu, đồng đều. Hệ vi hạt thể hiện khả năng giải phóng hoạt chất phụ thuộc pH: giải phóng nhanh tại pH 1,2, chậm và ổn định ở pH 6,8 và 7,4, phù hợp với các dạng bào chế theo đường uống. Ngoài ra, hệ NM/PLGA-NPs có khả năng trung hòa gốc tự do DPPH bền vững trong 72 giờ, điều này cho thấy hiệu quả kháng oxy hóa kéo dài. Việc đánh giá độc tính trên tế bào RAW264.7 cho thấy hệ vi hạt an toàn, không gây độc và có khả năng bảo vệ tế bào khỏi tổn thương stress oxy hóa do H2O2. Những kết quả này khẳng định tiềm năng của hệ vi hạt NM/PLGA-NPs trong ứng dụng điều trị các bệnh lý liên quan đến stress oxy hóa.
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