RNA CAN THIỆP LÊN GEN VP28 CỦA WSSV BIỂU HIỆN BẰNG BACULOVIRUS TÁI TỔ HỢP TRÊN TẾ BÀO CÔN TRÙNG

Đặng Thị Hoàng Oanh * , Marielle Van Hunten và Peter John Waklker

* Tác giả liên hệ (dthoanh@ctu.edu.vn)

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Ngày nhận bài: 20-04-2021
Ngày duyệt đăng: 03-06-2022
Ngày xuất bản: 01-05-2010
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Oanh, Đ. T. H., Hunten, M. V., & Waklker, P. J. (2010). RNA CAN THIỆP LÊN GEN VP28 CỦA WSSV BIỂU HIỆN BẰNG BACULOVIRUS TÁI TỔ HỢP TRÊN TẾ BÀO CÔN TRÙNG. Tạp chí Khoa học Đại học cần Thơ, (15a), 214-223. Truy vấn từ https://ctujsvn.ctu.edu.vn/index.php/ctujsvn/article/view/799
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Số. 15a (2010)
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Abstract

RNA interference (RNAi) has been widely used to inhibit virus infection. To target White spot syndrome virus (WSSV) in shrimp using RNAi, envelop protein gene VP28 was selected. To test if high level expression of the target gene could be inhibited using RNAi, the efficacy of selected dsRNAs were tested in insect cells using recombinant baculovirus expressing the WSSV VP28 from the polyhedrin promoter. Efficient silencing was demonstrated for gene target with levels of knock-down ranging from 80-100%.
Keywords: WSSV, dsRNA

Tóm tắt

RNA can thiệp (RNAi) hiện đang được sử dụng rất phổ biến để ức chế sự lây nhiễm của vi-rút. Gen VP28 của WSSV được chọn làm gen mục tiêu để ức chế sự lây nhiễm WSSV ở tôm bằng RNAi. Để đánh giá khả năng can thiệp của RNAi lên gen mục tiêu có biểu hiện ở mức độ cao, hiệu quả ức chế của dsRNA lên gen VP28 biểu hiện bằng baculovirus tái tổ hợp từ khởi điểm polyhedrin trên tế bào côn trùng được xác định. Kết quả cho thấy hiệu quả ức chế gen mục tiêu đạt được ở mức 80-100%.
Từ khóa: RNAi, WSSV, dsRNA

Article Details

Tài liệu tham khảo

Agrawal, N., Malhotra, P., Bhatnagar, R.K., 2004. SiRNA-directed silencing of transgene expressed in cultured insect cells. Biochemical and Biophysical Research Communications 320, 428-434.

Bernstein, E., Caudy, A. A., Hammond, S., and Hannon, G. J. 2001. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363-366.

Cherbas, L., Moss, R., Cherbas, P., 1994. Transformation techniques for Drosophila cell lines. In: Goldstein, L.S.B., Fyrberg, E.A. (Eds.). Methods in Cell Biology. Academic Press, San Diego.

Dang Thi Hoang Oanh, 2008. Inhibition of viral infection by using RNA interference. PhD thesis. Department of Molecular and Microbial Sciences. The University of Queensland. Australia.

Haasnoot, P.C.J., Cupac, D., Berkhout, B., 2003. Inhibition of virus replication by RNA interference. Journal of Biomedical Science 10, 607-616.

Kovacs, G.R., Guarino, L.A., Summers, M.D., 1991. Novel regulatory properties of the IE1 and IE0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus. Journal of virology 65, 5281-5288.

Leung, R.K.M., Whittaker, P.A., 2005. RNA interference: from gene silencing to gene-specific therapeutics. Pharmacology & Therapeutics 107, 222-239.

Means, J.C., Muro, I., Clem, R.J., 2003. Silencing of the baculovirus Op-iap3 gene by RNA interference reveals that it is required for prevention of apoptosis during Orgyia pseudotsugata M nucleopolyhedrovirus infection of Ld652Y cells. J Virol 77, 4481-4488.

Monsma, S.A., Oomens, A.G.P., Blissard, G.W., 1996. The GP64 Envelope Fusion Protein Is an Essential Baculovirus Protein Required for Cell-to-Cell Transmission of Infection. Journal of Virology 70, 4607-4616.

Nadala, E.C.B., Tapay, L.M., Loh, P.C., 1998. Characterization of a non-occluded baculovirus-like agent pathogenic to penaeid shrimp. Diseases of Aquatic Organisms 33, 221-229.

Reed, L.J., Muench, H., 1938. A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27, 493-497.

Schneider, I., Blumenthal, A.B., pp. . ase. 399, 166–169., 1978. Drosophila cell and tissue culture. In: Ashburner, M., Wright, T.R.F. (Eds.), The Genetics and Biology of Drosophila. Academic Press, London. 265–315.

Stram, Y., Kuzntzova, L., 2006. Inhibition of viruses by RNA interference. Virus Genes 32, 299-306.

Summers, M.D., Smith, G.E., 1987. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin No. 1555.

Tsai, J.M., Wang, H.C., Leu, J.H., Wang, A.H.J., Zhuang, Y., Walker, P.J., Kou, G.H., Lo, C.F., 2006. Identification of the nucleocapsid, tegument, and envelope proteins of the shrimp white spot syndrome virus virion. Journal of Virology 80, 3021-3029.

Valdes, V.J., Sampieri, A., Sepulveda, J., Vaca, L., 2003. Using Double-stranded RNA to Prevent in Vitro and in Vivo Viral Infections by Recombinant Baculovirus. J Biol Chem 278, 19317-19324.

van Hulten, M. C. W., Reijns, M., Vermeesch, A. M. G., Zandbergen, F. & Vlak, J. M. (2002). Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. Journal ofGeneral Virology 83, 257-265.

van Hulten, M.C.W., Witteveldt, J., Snippe, M., Vlak, J.M., 2001. White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology 285, 228-233.

Vlak, J.M., Bonami, J.R., Flegel, T.W., Kou, G.H., Lightner, D.V., Lo, C.F., Loh, P.C., Walker, P.J., 2005. Nimaviridae. In Virus Taxonomy - Eight report of the international committee on taxonomy of viruses, pp. 187-192. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier Academic Press.

Westenberg, M., Heinhuis, B., Zuidema, D.and Vlak, J. M. (2005). siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus. Virus Research 114, 133-9.

Wongteerasupaya, C., Vickers, J.E., Sriurairatana, S., Nash, G.L., Akarajamorn, A., Boonsaeng, V., Panyim, S., Tassanakajon, A., Withyachumnarnkul, B., Flegel, T.W., 1995. A Non-Occluded, Systemic Baculovirus That Occurs in Cells of Ectodermal and Mesodermal Origin and Causes High Mortality in the Black Tiger Prawn Penaeus-Monodon. Diseases of Aquatic Organisms 21, 69-77.

Yang, F., He, J., Lin, X.H., Li, Q., Pan, D., Zhang, X.B., Xu, X., 2001. Complete genome sequence of the shrimp white spot bacilliform virus. Journal of Virology 75, 11811-11820.