Chế tạo nhũ tương nano dầu neem kết hợp nano silica bằng phương pháp điểm đảo pha và đánh giá hiệu quả kháng nấm Pyricularia oryzae
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Abstract
This study aimed to prepare neem oil nanoemulsion by emulsion phase inversion method, incoporated silica nanoparticles and determine their antifungal efficacy against Pyricularia orizae. We performed factor affecting the formation nanoemulsion including oil neem to surfactant ratio, surfactant ratio, neem oil ratio, silica nanoparticles ratio and selected the best formula to evaluate the physical chemical indicators. The results showed that the best nanoemulsion with a composition of 20% neem oil, 20% Tween 80, and 60% distilled water, 0.5% silica nanoparticles. The results of particle size, polydispersity index, viscosity, turbidity, and pH of the formulation were 154.8 nm; 0.233; 21.5 cP; 51.18 cm-1; 6.2, respectively, and were stable when kept at room temperature after two months of storage. The results showed nanoemulsion good antifungal activity and inhibitory efficacy of 80%; 78.26%; 76.02% at 100 µL/mL concentration after 3, 6, 9 days. This study suggests that neem oil nanoemulsion could be a potential to replace chemical synthetic fungicides.
Tóm tắt
Nghiên cứu nhằm mục đích tạo nhũ tương nano dầu neem bằng phương pháp điểm đảo pha, kết hợp với nano silica và xác định hiệu quả kháng nấm Pyricularia orizae. Khảo sát các yếu tố ảnh hưởng đến quá trình tạo hệ nhũ như tỷ lệ dầu neem và chất hoạt động bề mặt, tỷ lệ chất hoạt động bề mặt, tỷ lệ dầu neem, tỷ lệ nano silica và chọn được công thức tốt nhất đánh giá các chỉ tiêu hóa lý. Kết quả hệ nhũ tương tốt nhất với thành phần bao gồm 20% dầu neem, 20% Tween 80 và 60% nước cất, 0,5% nano silica. Kết quả kích thước hạt trung bình, chỉ số đa phân tán, độ nhớt, độ đục, pH của hệ nhũ lần lượt là 154,8 nm; 0,233; 21,5 cP; 51,18 cm-1; 6,2 và ổn định ở nhiệt độ phòng sau 2 tháng bảo quản. Kết quả nhũ tương cho thấy hoạt tính kháng nấm tốt và hiệu quả ức chế đạt 80%; 78,26%; 76,02% ở nồng độ 100 µL/mL sau 3,6,9 ngày. Nghiên cứu cho thấy nhũ tương có tiềm năng thay thế cho thuốc diệt nấm tổng hợp hóa học.
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