Khảo sát sự thay đổi của một số chỉ tiêu sinh lý – sinh hoá máu do Leptospira gây ra trên chó tại trạm chẩn đoán xét nghiệm và điều trị bệnh động vật thành phố Hồ Chí Minh
Abstract
Serum sample of 257 dogs were test using a microscopic agglutination test (MAT) with a standard antigen set of 24 serovars. The prevalence of Leptospira in dogs was 9.34%. The study identified 7 Lepstopira serovars, of which the common serovars were L. interrogans serovar Bataviae (46.67%). At a titer of 1/400, dogs were infected with 4 out of 24 Leptospira serovars, with infection rates ranging from 28.57% to 100%. At titers of 1/800 and 1/1600, dogs were infected with only 1 out of 24 Leptospira serovars (bataviae serovar), with the lowest infection rate of 7.14%. Blood physiological indexes showed that red blood cells (5.38 1012/L) and hematocrit (33.64 %) were lower than those of normal; white blood cells and lymphocytes following 15.23 x 109/L and 23.10% higher than normal ranges. The average blood biochemical indexes were higher than those of normal, AST was 74.48 U/L, ALT was 91.48 U/L, protein total was 7.85 g/dl, albumin was 3.94 g/dl, B.U.N was 32.13 mg/dL and ALP was 216,09 U/L. The prevalence of Leptospira in dogs raises the risk of transmission to humans.
Tóm tắt
Phản ứng vi ngưng kết (MAT) với bộ kháng nguyên chuẩn gồm 24 serovar được sử dụng để xét nghiệm 257 mẫu huyết thanh trên chó. Tỷ lệ nhiễm Leptospira trên chó là 9,34%. Nghiên cứu đã xác định được 7 serovar, trong đó L. interrogans serovar Bataviae chiếm chủ yếu (46,67%). Ở hiệu giá ngưng kết 1/400, chó nhiễm 4/24 serovar Leptospira tỷ lệ từ 28,57% đến 100%. Ở hiệu giá ngưng kết 1/800 và 1/1600, chó nhiễm 1/24 serovar Leptospira ở serovar bataviae và có tỷ lệ thấp nhất 7,14%. Chỉ số sinh lý máu cho thấy, giá trị trung bình của hồng cầu (5,38 1012/L) và hematocrit (33,64 %) giảm, hàm lượng bạch cầu tổng số và bạch cầu lympho tăng theo thứ tự là 15,23 x 109/L và 23,10%. Trung bình các chỉ số sinh hóa cao hơn bình thường, chỉ số AST là 74,48 U/L, chỉ số ALT là 91,48 U/L, protein tổng số là 7,85 g/dl; albumin là 3,94 g/dl; chỉ số B.U.N là 32,13 mg/dL và ALP là 216,09 U/L. Sự lưu hành của Leptospira trên chó cảnh báo nguy cơ truyền lây sang người.
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