Ảnh hưởng của sucrose và lecithin đến chất lượng tinh trùng thỏ đen Việt Nam bảo quản trong nitơ lỏng
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Abstract
The objective of the study was to determine the appropriate concentrations of sucrose and lecithin during cryopreservation of Vietnamese black rabbit sperm. In experiment 1, semen was diluted with Tris Citrate Glucose stored medium supplemented with 15% egg yolk and sucrose at 4 concentrations: 0M, 0.05M, 0.1M and 0.25M. In experiment 2, egg yolks were replaced with lecithin in the stored medium at concentrations: 1%, 1.5% and 2%. The samples was transferred to straws and stored in liquid nitrogen (-196ᵒC). After 72 hours of storage, sperm quality test results showed that 0.05M sucrose combined with 1.5% lecithin helps sperm maintain the best quality with overall motility rate, viablity rate, membrane integrity, acrosome integrity and antioxidant capacity were 56.39%, 63.74%, 49.04%, 64.43%, 20.41%, respectively. This study showed that combining 0.05M sucrose and 1.5% lecithin in cryoperservation is important in breeding and preserving Vietnamese black rabbits.
Tóm tắt
Mục tiêu của nghiên cứu là xác định nồng độ sucrose và lecithin phù hợp trong quá trình bảo quản lạnh tinh trùng thỏ đen Việt Nam. Ở thí nghiệm 1, tinh dịch được pha loãng với môi trường bảo quản Tris Citrate Glucose có bổ sung 15% lòng đỏ trứng và sucrose ở 4 nồng độ: 0M, 0,05M, 0,1M và 0,25M. Ở thí nghiệm 2, lòng đỏ trứng được thay thế bằng lecithin trong môi trường bảo quản ở các nồng độ: 1%, 1,5% và 2%. Mẫu được chuyển vào ống trữ tinh và được trữ trong nitơ lỏng (-196ᵒC). Sau 72 giờ được bảo quản, kết quả kiểm tra chất lượng tinh trùng cho thấy, 0,05M sucrose kết hợp với 1,5% lecithin giúp tinh trùng giữ được chất lượng tốt nhất với tỷ lệ di động tổng số, tỷ lệ sống, tính toàn vẹn màng tế bào, toàn vẹn acrosome và khả năng kháng oxy hóa lần lượt 56,39%, 63,74%, 49,04%, 64,43%, 20,41%. Nghiên cứu cho thấy việc kết hợp 0,05M sucrose và 1,5% lecithin trong môi trường bảo quản lạnh có ý nghĩa quan trọng trong nhân giống và bảo tồn thỏ đen Việt Nam.
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Tài liệu tham khảo
Abdel, K. K., Gabr, S., E. Younan, G., M. Shehab El-Din, Ahmad., & Ghodaia, A. E. B. (2024). Variables of cryopreserved rabbit spermatozoa as affected by nano-curcumin supplementation into Tris-extender containing egg yolk or soybean lecithin extender. Journal of Sustainable Agricultural and Environmental Sciences, 3(1), 10–17. https://doi.org/10.21608/jsaes.2024.254166.1068
Agha, R. A., Khalili M., Nabi A., & Ashourzadeh S. (2014). Vitrification is not superior to rapid freezing of normozoospermic spermatozoa: effects on sperm parameters, DNA fragmentation and hyaluronan binding. BioMedicine Online, 28, 352-58. https://doi.org/10.1016/j.rbmo.2013.11.015
Amann R.P. (1999). Cryopreservation of sperm. In: Knobil E, Neill JD (eds) Encyclopedia of reproduction. Academic Press, Burlington, MA, pp. 773–783.
Arando, A., Gonzalez, A., Delgado, J. V., Arrebola, F. A., & Perez-Marín, C. C. (2017). Storage temperature and sucrose concentrations affect ram sperm quality after vitrification. Animal Reproduction Science, 181, 175–185. https://doi.org/10.1016/j.anireprosci.2017.04.008
Bousseau, S., Brillard, J. P., Marquant-Le Guienne, B., Guérin, B., Camus, A., & Lechat, M. (1998). Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents. Theriogenology, 50(5), 699–706. https://doi.org/10.1016/S0093-691X(98)00175-7
Chelucci S., Pasciu V., Succu S., Addis D., Leoni G.G., Manca M.E., Naitana S., & Berlinguer F. (2015). Soybean lecithin-based extender preserves spermatozoa membrane integrity và fertilizing potential during goat semen cryopreservation. Theriogenology, 83(6), 1064-74.
Consuegra, C., Crespo, F., Bottrel, M., Ortiz, I., Dorado, J., Diaz-Jimenez, M., Pereira, B., & Hidalgo, M. (2018). Stallion sperm freezing with sucrose extenders: A strategy to avoid permeable cryoprotectants. Animal Reproduction Science, 191, 85–91. https://doi.org/10.1016/j.anireprosci.2018.02.013
Curry M.R., Redding B.J., & Watson P.F. 1995. Determination of water permeability coefficient and its activation energy for rabbit spermatozoa. Cryobiology, 32, 175–181
Crowe, J. H., Crowe, L. M., Carpenter, J. F., Rudolph, A. S., Wistrom, C. A., Spargo, B. J., & Anchordoguy, T. J. (1988). Interactions of sugars with membranes. Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 947(2), 367–384. https://doi.org/10.1016/0304-4157(88)90015-9
Dalmazzo, A., Losano, J. D. A., Rocha, C. C., Tsunoda, R. H., Angrimani, D. de S. R., Mendes, C. M., Assumpção, M. E. O. D., Nichi, M., & Barnabe, V. H. (2018). Effects of Soy Lecithin Extender on Dog Sperm Cryopreservation. Animal Biotechnology, 29(3), 174–182. https://doi.org/10.1080/10495398.2017.1334662
Diaz-Jimenez, M., Dorado, J., Ortiz, I., Consuegra, C., Pereira, B., Gonzalez-De Cara, C. A., Aguilera, R., Mari, G., Mislei, B., Love, C. C., & Hidalgo, M. (2018). Cryopreservation of donkey sperm using non-permeable cryoprotectants. Animal Reproduction Science, 189, 103–109. https://doi.org/10.1016/j.anireprosci.2017.12.013
El-Sisy, G. A., Shahba, M. I., & El-Sheshtawy, R. I. (2016). Freezability of buffalo semen with TRIS extender enriched with disaccharides (trehalose or sucrose) and different glycerol concentrations. Asian Pacific Journal of Reproduction, 5(5), 416–418.
Fumuso F.G., Giulianob S.M., Chavesa M.G., Neilda D.M., Miragayaa M.H., Gambarottac M.C., Carreteroa, M.I. (2018). Seminal plasma affects the survival rate and motility pattern of raw llama spermatozoa. Anim. Reprod. Sci., 192, 99-106. https://doi.org/10.1016/j.anireprosci.2018.02.019
Iaffaldano, N., Di Iorio, M., Rosato, M. P., & Manchisi, A. (2014). Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose. Animal Reproduction Science, 151(3–4), 220–228. https://doi.org/10.1016/j.anireprosci.2014.10.020
Khan, I. M., Cao, Z., Liu, H., Khan, A., Rahman, S. U., Khan, M. Z., Sathanawongs, A., & Zhang, Y. (2021). Impact of Cryopreservation on Spermatozoa Freeze-Thawed Traits and Relevance OMICS to Assess Sperm Cryo-Tolerance in Farm Animals. Frontiers in Veterinary Science, 8. https://doi.org/10.3389/fvets.2021.609180
Khuong, T. T. T., Duy, N. L. K., Hang, N. T., Ngoc, P. K., & Tuyen, D. N. D. (2023a). Improving indigenous Vietnamese Black Rabbit frozen sperm quality: the role of glycine and sperm selection methods. World Rabbit Science, 31(4), 229–236. https://doi.org/10.4995/wrs.2023.19690
Khuong T. T. T., Nga T. T., Duy N. L. K. & Trung T. T. (2023b). Improving rabbit sperm quality during cold storage preservation through cyteine supplementation. Journal of Animal Husbandry Sciences and Technics, 291, 11-18
Kubovicova, E., Makarevich, A. V., Balazi, A., Vasicek, J., & Chrenek, P. (2022). Factors affecting rabbit sperm cryopreservation: a mini-review. Zygote, 30(1), 1–8. https://doi.org/10.1017/S0967199421000137
Kumar, S., Sahni, K.L., and Mohan, G. (1992). Effect of different levels of glycerol and yolk on freezing and storage of buffalo semen in milk, tris and sodium citrate buffers. Buffalo J., 2, 151–156
Mahiddine, F. Y., & Kim, M.-J. (2021). Overview on the Antioxidants, Egg Yolk Alternatives, and Mesenchymal Stem Cells and Derivatives Used in Canine Sperm Cryopreservation. Animals, 11(7), 1930. https://doi.org/10.3390/ani11071930
Masoudi, R., Sharafi, M., Zareh Shahneh, A., Towhidi, A., Kohram, H., Esmaeili, V., Shahverdi, A., & Davachi, N. D. (2016). Fertility and flow cytometry study of frozen-thawed sperm in cryopreservation medium supplemented with soybean lecithin. Cryobiology, 73(1), 69–72. https://doi.org/10.1016/j.cryobiol.2016.05.010
Mocé, E., & Vicente, J. S. (2009). Rabbit sperm cryopreservation: A review. Animal Reproduction Science, 110(1–2), 1–24.
Moura, T. C. M., Arruda, L. C. P., Araújo Silva, R. A. J., Silva, R. P. F., Oliveira, A. S., Tobal, L. F. M., Batista, A. M., Carneiro, G. F., & Guerra, M. M. P. (2022). Diluent Containing Dimethylformamide Added With Sucrose Improves In Vitro Quality After Freezing/Thawing Stallion Sperm. Journal of Equine Veterinary Science, 109, 103825. https://doi.org/10.1016/j.jevs.2021.103825
National Research Council. (1977). Nutrient Requirements of Rabbits, 2th ed. National Academy of Sciences. Washington DC, USA
Nenadis, N., Wang, L. F., Tsimidou, M. & Zhang, H. Y. (2004). Estimation of scavenging activity of phenolic compounds using the ABTS (*+) assay. J Agric Food Chem, 52(15), 4669-74.
Nishijima, K., Kitajima, S., Koshimoto, C., Morimoto, M., Watanabe, T., Fan, J., & Matsuda, Y. (2015). Motility and fertility of rabbit sperm cryopreserved using soybean lecithin as an alternative to egg yolk. Theriogenology, 84(7), 1172–1175. https://doi.org/10.1016/j.theriogenology.2015.06.018
Ozimic S., Ban-Frangez H., & Stimpfel M. (2023). Sperm Cryopreservation Today: Approaches, Efficiency, and Pitfalls. Curr. Issues Mol. Biol., 45: 4716-4734. https://doi.org/10.3390/cimb45060300
Ozkavukcu, S., Erdemli, E., Isik, A., Oztuna, D., & Karahuseyinoglu, S. (2008). Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. Journal of Assisted Reproduction and Genetics, 25(8), 403–411. https://doi.org/10.1007/s10815-008-9232-3
Pickett, B. W. (1971). Factors affecting the utilization of frozen bovine semen for maximum reproductive efficiency. A.I. Dig. 19, 8.
Prihantoko, K. D., Yuliastuti, F., Haniarti, H., Kusumawati, A., Widayati, D. T., & Budiyanto, A. (2020). The Acrosome Integrity Examination of Post-thawed Spermatozoa of Several Ongole Grade Bull in Indonesia Using Giemsa Staining Method. IOP Conference Series: Earth and Environmental Science, 478(1), 012042. https://doi.org/10.1088/1755-1315/478/1/012042
Ramu, S. & Jeyendran, R. S. (2013). The hypo-osmotic swelling test for evaluation of sperm membrane integrity. Methods Mol. Biol. 927, 21–25. https://doi.org/10.1007/978-1-62703-038-0_3
Sánchez, R., Risopatrón, J., Schulz, M., Villegas, J., Isachenko, V., Kreinberg, R., & Isachenko, E. (2011). Canine sperm vitrification with sucrose: Effect on sperm function. Andrologia, 43(4), 233–241.
https://doi.org/10.1111/j.1439-0272.2010.01054.x
Serrano A. M., Aquilina, M. C., Zak, L. J., Ellis, P. J., & Griffin, D. K. (2023). Successful recovery of motile and viable boar sperm after vitrification with different methods (pearls and mini straws) using sucrose as a cryoprotectant. Cryobiology, 113. https://doi.org/10.1016/j.cryobiol.2023.104583
Sharafi, M., Borghei-Rad, S. M., Hezavehei, M., Shahverdi, A., & Benson, J. D. (2022). Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies. Animals, 12(23), 3271. https://doi.org/10.3390/ani12233271
Silagadze, D. (2022). Major Challenges of Agriculture. Georgian scientists. https://doi.org/10.52340/gs.2022.04.02.11
Sun, L., Fan, W., Wu, C., Zhang, S., Dai, J., & Zhang, D. (2020). Effect of substituting different concentrations of soybean lecithin and egg yolk in tris-based extender on goat semen cryopreservation. Cryobiology, 92, 146–150. https://doi.org/10.1016/j.cryobiol.2019.12.004
Younan, G., Shehab El-Din, A., Abdel, K. A., El-Sherbieny, M., & Helmy, A. (2021). Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen. Journal of Animal and Poultry Production, 12(1), 47–54. https://doi.org/10.21608/jappmu.2021.149460