Khả năng kháng vi sinh vật của cao chiết từ một số giống cam vỏ xanh thu tại Đồng bằng sông Cửu Long và giống cam vỏ vàng nhập ngoại
Abstract
The study compared the antibacterial effectiveness of extracts from three green-skinned orange varieties (Sanh, Xoan and Mat oranges) and one yellow-skinned orange variety (Navel orange) by soaking and Soxhlet. The antibacterial activity of the extract was evaluated on Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. In both extraction methods and five microbial strains surveyed, extracts from green-skinned orange varieties have better antibacterial activity than yellow-skinned orange varieties, in which Xoan orange extract has the strongest antibacterial activity. The extract extracted by soaking has higher antimicrobial activity than th at extracted by Soxhlet. In addition, the extracts showed more effective antibacterial properties against Gram-positive bacteria (B. cereus, S. aureus) and fungi (C. albicans) than Gram-negative bacteria (P. aeruginosa, P. aeruginosa, E coli). In summary, the extract of Xoan orange by soaking has the strongest antibacterial properties, especially effective against S. aureus and C. albicans.
Tóm tắt
Nghiên cứu được thực hiện nhằm so sánh hiệu quả kháng vi sinh của cao chiết từ ba giống cam vỏ xanh (cam Sành, Xoàn và Mật) và một giống cam vỏ vàng (cam Navel) bằng ngâm dầm và Soxhlet. Hoạt tính kháng vi sinh của cao chiết được đánh giá trên vi khuẩn Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli và nấm Candida albicans. Ở cả hai phương pháp trích ly và 5 chủng vi sinh được khảo sát, cao chiết từ các giống cam vỏ xanh có hoạt tính kháng vi sinh tốt hơn so với giống cam vỏ vàng, trong đó cao chiết cam Xoàn có hoạt tính kháng vi sinh mạnh nhất. Cao chiết bằng ngâm dầm có hoạt tính khánh sinh cao hơn so với cao chiết bằng Soxhlet. Ngoài ra, các loại cao chiết thể hiện khả năng kháng vi sinh hiệu quả hơn đối với vi khuẩn Gram dương (B. cereus, S. aureus) và nấm (C. albicans) so với vi khuẩn Gram âm (P. aeruginosa, E. coli). Tóm lại, cao chiết cam Xoàn bằng ngâm dầm kháng vi sinh mạnh nhất, đặc biệt hiệu quả đối với S. aureus và C. albicans.
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Tài liệu tham khảo
Baba, J., Mohammed, S. B., Ya’aba, Y., & Umaru, F. I. (2018). Antibacterial Activity of Sweet Orange Citrus sinensis on some Clinical Bacteria Species Isolated from Wounds. J Family Med Community Health, 5(4), 1154. https://doi.org/10.47739/2379-0547/1154
Biosci, I. J., Rad, J. S., Mahsan, S., Alfatemi, H., Rad, M. S., & Biosci, I. J. (2014). In vitro assessment of antibacterial activity of Salicornia herbacea L. seed extracts against multidrug resistant grampositive and gram-negative bacteria. International Journal of Biosciences (IJB), 6655, 217–222. https://doi.org/10.12692/ijb/4.6.217-222
Górski, M., Niedźwiadek, J., & Magryś, A. (2022). Antibacterial activity of curcumin – a natural phenylpropanoid dimer from the rhizomes of Curcuma longa L. and its synergy with antibiotics. Annals of Agricultural and Environmental Medicine, 29(3), 394–400. https://doi.org/10.26444/aaem/148393
Hanafy, S. M., Abd El-Shafea, Y. M., Saleh, W. D., & Fathy, H. M. (2021). Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms. Journal of Genetic Engineering and Biotechnology, 19(1), 80. https://doi.org/10.1186/s43141-021-00151-0
Hernández, T., Canales, M., Avila, J. G., García, A. M., Martínez, A., Caballero, J., Vivar, A. R. de, & Lira, R. (2005). Composition and antibacterial activity of essential oil of Lantana achyranthifolia Desf. (Verbenaceae). Journal of Ethnopharmacology, 96(3), 551–554. https://doi.org/10.1016/j.jep.2004.09.044
Hussain, K. A. mja., Tarakji, B., Kandy, B. P. urushothama. P., John, J., Mathews, J., Ramphul, V., & Divakar, D. D. evan. (2015). Antimicrobial effects of citrus sinensis peel extracts against periodontopathic bacteria: an in vitro study. Roczniki Panstwowego Zakladu Higieny, 66(2), 173–178. http://www.ncbi.nlm.nih.gov/pubmed/26024407
Jiménez-Castro, M. P., Buller, L. S., Sganzerla, W. G., & Forster‐Carneiro, T. (2020). Bioenergy production from orange industrial waste: a case study. Biofuels, Bioproducts and Biorefining, 14(6), 1239–1253. https://doi.org/10.1002/bbb.2128
Kunder, H., Gurusiddappa, L. H., Hanumanthappa, B. S., & Kalikeri, S. (2022). Antimicrobial Activity and Phytochemical Analysis of Solvent Extraction of Citrus reticulata Peels. World Journal of Environmental Biosciences, 11(2), 20–25. https://doi.org/10.51847/g5wDo6EASN
Kwieciński, J., Eick, S., & Wójcik, K. (2009). Effects of tea tree (Melaleuca alternifolia) oil on Staphylococcus aureus in biofilms and stationary growth phase. International Journal of Antimicrobial Agents, 33(4), 343–347. https://doi.org/10.1016/j.ijantimicag.2008.08.028
Li, W., Li, Y., Bi, J., Ji, Q., Zhao, X., Zheng, Q., Tan, S., & Gao, X. (2020). Effect of hot air drying on the polyphenol profile of Hongjv (Citrus reticulata Blanco, CV. Hongjv) peel: A multivariate analysis. Journal of Food Biochemistry, 44(5), 1–10. https://doi.org/10.1111/jfbc.13174
McKay, S., Sawant, P., Fehlberg, J., & Almenar, E. (2021). Antimicrobial activity of orange juice processing waste in powder form and its suitability to produce antimicrobial packaging. Waste Management, 120, 230–239. https://doi.org/10.1016/j.wasman.2020.11.048
Mitsagga, C., Petrotos, K., & Giavasis, I. (2021). Antimicrobial Properties of Lyophilized Extracts of Olive Fruit, Pomegranate and Orange Peel Extracts against Foodborne Pathogenic and Spoilage Bacteria and Fungi In Vitro and in Food Matrices. Molecules, 26(22), 7038. https://doi.org/10.3390/molecules26227038
Mostafa, R., & Essawy, H. (2021). Screening and Quantification of Bioactive Compounds and Antimicrobial Activities of Fresh Juice, Methanolic Peel and Pulp Extract of Citrus sinensis L. (Sweet Orange). Egyptian Academic Journal of Biological Sciences, G. Microbiology, 13(2), 1–10. https://doi.org/10.21608/eajbsg.2021.189671
Niawanti, H., Lewar, Y. S., & Octavia, N. N. (2019). Effect of Extraction Time on Averrhoa bilimbi Leaf Ethanolic Extracts Using Soxhlet Apparatus. IOP Conference Series: Materials Science and Engineering, 543(1), 012018. https://doi.org/10.1088/1757-899X/543/1/012018
Oikeh, E. I., Oviasogie, F. E., & Omoregie, E. S. (2020). Quantitative phytochemical analysis and antimicrobial activities of fresh and dry ethanol extracts of Citrus sinensis (L.) Osbeck (sweet Orange) peels. Clinical Phytoscience, 6(1), 46. https://doi.org/10.1186/s40816-020-00193-w
Saleem, M., & Saeed, M. T. (2020). Potential application of waste fruit peels (orange, yellow lemon and banana) as wide range natural antimicrobial agent. Journal of King Saud University - Science, 32(1), 805–810. https://doi.org/10.1016/j.jksus.2019.02.013
Satari, B., & Karimi, K. (2018). Citrus processing wastes: Environmental impacts, recent advances, and future perspectives in total valorization. Resources, Conservation and Recycling, 129(October 2017), 153–167. https://doi.org/10.1016/j.resconrec.2017.10.032
Sharma, K., Mahato, N., Cho, M. H., & Lee, Y. R. (2017). Converting citrus wastes into value-added products: Economic and environmently friendly approaches. Nutrition, 34, 29–46. https://doi.org/10.1016/j.nut.2016.09.006
Shehata, M. G., Awad, T. S., Asker, D., El Sohaimy, S. A., Abd El- Aziz, N. M., & Youssef, M. M. (2021). Antioxidant and antimicrobial activities and UPLC-ESI-MS/MS polyphenolic profile of sweet orange peel extracts. Current Research in Food Science, 4(May), 326–335. https://doi.org/10.1016/j.crfs.2021.05.001
Silva, M. C., Matos, A. F., Santos, H. L. C. dos, Gomes, J. V., Pastura, D. G. N., Pereira, G. L., Rocha, E. B. da, Larangeira, M. de J. C., Alves, R. S., Bastos, L. de O., Borba, H. R., & Lima, V. M. de. (2020). Laurus nobilis L.: assessment of the cytotoxic and genotoxic potential of aqueous extracts by micronucleus and Allium cepa assays. Brazilian Journal of Pharmaceutical Sciences, 56(March). https://doi.org/10.1590/s2175-97902019000318302
Yohanes, R., Geremew, T., Tafese, T., & Endale Annisa, M. (2023). Antibacterial and antioxidant activity of compounds from Citrus sinensis peels and in silico molecular docking study. International Journal of Secondary Metabolite, 10(3), 437–458. https://doi.org/10.21448/ijsm.1180610
Zahoor, S., Anwar, F., Mehmood, T., Sultana, B., & Qadir, R. (2016). Variation in antioxidant attributes and individual phenolics of citrus fruit peels in relation to different species and extraction solvents. Journal of the Chilean Chemical Society, 61(2), 2884–2889. https://doi.org/10.4067/S0717-97072016000200007