Khảo sát hoạt tính kháng oxy hóa, kháng viêm và kháng khuẩn của cây rau dệu (Alternanthera sessilis)
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Abstract
The present study was carried out to determine the phytochemistry and biological activity of extracts from the above-ground parts (stems and leaves), and the leaves and stems of Alternanthera sessilis. Antioxidant activity is determined based on neutralising or inhibiting abiotic free radicals. Anti-inflammatory activity was determined based on its ability to protect red blood cell membranes and inhibit bovine serum albumin denaturation. The antibacterial activity was determined by determining the antibacterial ring diameter, the minimum inhibitory concentration and the minimum bactericidal concentration. Alternanthera sessilis extracts have antioxidant and anti-inflammatory activities with EC50 values ranging from 50.60±0.29 to 125.58±7.12 µg/mL. Alternanthera sessilis extracts have the ability to inhibit Gram-positive bacteria more effectively than Gram-negative bacteria with minimum bactericidal concentrations ranging from 250 to 2,000 µg/mL. The antioxidant, anti-inflammatory and antibacterial activities of Alternanthera sessilis extracts are related to the polyphenols, flavonoids and alkaloids content. These results highlighted the potential use of Alternanthera sessilis extracts as a natural antioxidant, anti-inflammatory and antimicrobial agent.
Tóm tắt
Nghiên cứu được thực hiện để xác định hóa thực vật và hoạt tính sinh học của các cao thân lá, thân và lá rau dệu. Hoạt tính kháng oxy hóa được xác định dựa vào hoạt động trung hòa hoặc ức chế các gốc tự do phi sinh học. Hoạt tính kháng viêm được xác định dựa trên khả năng bảo vệ màng tế bào hồng cầu và ức chế sự biến tính protein. Hoạt tính kháng khuẩn được xác định dựa vào đường kính vòng kháng khuẩn, nồng độ ức chế tối thiểu, nồng độ diệt khuẩn tối thiểu. Các cao rau dệu có hoạt tính kháng oxy hóa, kháng viêm với giá trị EC50 dao động từ 50,60±0,29 đến 125,58±7,12 µg/mL. Các cao rau dệu ức chế vi khuẩn Gram dương hiệu quả hơn Gram âm, nồng độ diệt khuẩn tối thiểu dao động từ 250 đến 2.000 µg/mL. Hoạt động kháng oxy hóa, kháng viêm và kháng khuẩn của rau dệu có liên quan đến hàm lượng polyphenol, flavonoid và alkaloid. Kết quả này cho thấy tiềm năng sử dụng cao rau dệu như chất kháng oxy hóa, kháng viêm, kháng khuẩn tự nhiên.
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