Khảo sát sự hiện diện và định danh vi khuẩn Staphylococcus spp. trên chó viêm da tại thành phố Hồ Chí Minh
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Abstract
This study aims to determine the prevalence of Staphylococcus spp. in dogs with dermatitis visiting veterinary clinics in Ho Chi Minh city using clinical examination, bacterial isolation and identification by PCR technique. A total of 96 swab samples were collected from skin of dogs with dermatitis. The results showed that the isolation rate of Staphylococcus spp. from dogs with dermatitis was 84.4%. The presence of Staphylococcus spp. free-ranging and semi-confined dogs (94.7%) was higher than confined dogs (69.2%). The prevalence of Staphylococcus spp. in dogs under one year old (62.5%) was lower than in those over five years (96.7%). A total of 105 Staphylococcus strains were isolated in this study, with the prevalence of coagulase-positive Staphylococcus (80.0%) being higher than that of coagulase-negative Staphylococcus (20.0%). In addition, four species of Staphylococcus spp. including S. aureus, S. pseudintermedius, S. epidermidis, and S. schleiferi were identified and S. pseudintermedius was isolated at the highest rate (49.5%).
Tóm tắt
Khảo sát sự hiện diện vi khuẩn Staphylococcus spp. trên chó viêm da được thực hiện tại một số phòng khám ở thành phố Hồ Chí Minh thông qua phương pháp kiểm tra lâm sàng, phân lập vi khuẩn và định danh bằng kỹ thuật PCR. Tổng số 96 mẫu swab da được thu thập và định danh vi khuẩn Staphylococcus ở những chó có biểu hiện bệnh viêm da cho thấy tỷ lệ hiện diện Staphylococcus spp. là 84,4%. Tỷ lệ phát hiện Staphylococcus spp. ở chó nuôi thả và kết hợp giữa nuôi thả với nuôi nhốt (94,7%) cao hơn chó nuôi nhốt (69,2%); tỷ lệ phát hiện chó dưới một năm tuổi nhiễm Staphylococcus spp. (62,5%) thấp hơn chó trên 5 năm tuổi (96,7%). Ngoài ra, trong số 105 chủng Staphylococcus phân lập, vi khuẩn Staphylococcus spp. thuộc nhóm dương tính coagulase (80,0%) hiện diện với tỷ lệ cao hơn các loài Staphylococcus spp. thuộc nhóm âm tính coagulase (20,0%). Kết quả định danh vi khuẩn bằng phương pháp PCR cho thấy đã xác định được 4 loài Staphylococcus là S. aureus, S. pseudintermedius, S. epidermidis và S. schleiferi. Trong đó, S. pseudintermedius chiếm tỷ lệ cao nhất (49,5%).
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