Tối ưu hóa quy trình chiết xuất cao chiết giàu polyphenol và flavonoid có hoạt kháng vi khuẩn Vibrio spp. từ lá cây núc nác (Oroxylum indicum L.)
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Abstract
Response surface methodology was applied to examine the influence of the extraction parameters on the total polyphenols content (TPC), and total flavonoids content (TFC) of extracts from Oroxylum indicum L. leaves (OIL). The optimal conditions for TPC and TFC included the ultrasonic temperature of 59°C, the ethanol concentration of 69%, the ultrasonic time of 11 min, and the material/the solvent ratio of 1/25 (w/v). The verification of the experimental process was done at the previously defined optimal conditions. The experimental values (TPC=215.47±1.03 mg GAE/g extract; TFC=158.01±1.12 mg QE/g extract) showed good agreement with the predicted the values (TPC=214.09 mg GAE/g extract; TFC=158.77 mg QE/g extract). The antibacterial activity of the OIL optimal extract was investigated against five strains of Vibrio spp. (VC-1, VC-2, VC-3, VC-4, and VC-5). The OIL optimal extract exhibited strong antibacterial activity against Vibrio spp, with the minimum inhibitory concentration (MIC) ranging from 320 to 640 µg/mL. The study results also showed that the minimum bactericidal concentration (MBC) of the OIL optimal extract ranged from 640 to 1280 µg/mL.
Tóm tắt
Phương pháp đáp ứng bề mặt được áp dụng để kiểm tra ảnh hưởng của các yếu tố chiết xuất đến hàm lượng polyphenol (TPC) và flavonoid (TFC) trong lá núc nác (LNN). Điều kiện tối ưu được xác định để chiết xuất TPC và TFC là: nhiệt độ 59°C, ethanol 69%, thời gian 11 phút và tỷ lệ nguyên liệu/ dung môi là 1/25 (w/v). Quá trình chiết xuất được xác minh ở các điều kiện tối ưu đã được xác định. Các giá trị thực nghiệm (TPC=215,47±1,03 mg GAE/g cao chiết; TFC=158,01±1,12 mg QE/g cao chiết) cho thấy sự phù hợp tốt với giá trị dự đoán (TPC=214,09 mg GAE/g cao chiết; TFC=158,77 mg QE/g cao chiết). Hoạt tính kháng khuẩn của cao tối ưu LNN được nghiên cứu chống lại năm chủng Vibrio spp. (VC-1, VC-2, VC-3, VC-4, và VC-5). Cao tối ưu LNN có hoạt tính kháng khuẩn mạnh chống lại Vibrio spp. Nồng độ ức chế tối thiểu của cao tối ưu LNN dao động từ 320 đến 640 µg/mL. Kết quả nghiên cứu cho thấy nồng độ diệt khuẩn tối thiểu của cao tối ưu LNN dao động từ 640 đến 1280 µg/mL.
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