Sàng lọc phân đoạn tảo nâu Dictyopteris polypodioides có khả năng ức chế enzyme -amylase và -glucosidase
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Abstract
The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. 90% MeOH extract of Dictyopteris polypodioides exhibited the strongest α-amylase and α-glucosidase inhibitory with IC50 (52.95±0.28 mgL-1, 88.04±0.05 mgL-1), respectively, non-different with Acarbose (60.88±0.48 mgL-1, 92.16±1.67 mgL-1). Using a silica chromatography column, collecting Fr.2 with potential inhibitory. At a concentration of 0.1 mg/mL, Fr.2 showed remarkable inhibitory of α-amylase and α-glucosidase up to 87.49% và 93.43%, respectively. Fr.2 was constitutively fractionated by the ODS column, separated into Fr.2.1, Fr.2.2, and Fr.2.3. The result illustrated that Fr.2.2 showed excellent inhibitory activity against α-amylase (IC50=26.14 mgL-1) and α-glucosidase (IC50=21.38 mgL-1), significantly higher 3 and 4 times than Acarbose. Analysis of the possible compound in Fr2.2 by 1H-NMR and 13C-NMR, the chemical structure was elucidated as zonarol compound. Therefore, this study suggested that zonarol is the main contribution in Brown algae Dictyopteris polypodioides to controlling glucose postprandial in diabetes patients.
Tóm tắt
Sử dụng hoạt chất tự nhiên có khả năng ức chế enzyme chuyển hóa carbohydrate như α-amylase và α-glucosidase, là một trong những liệu pháp hiệu quả trong điều trị bệnh đái tháo đường (ĐTĐ). Cao chiết 90% MeOH của Tảo nâu Dictyopteris polypodioides cho hiệu quả ức chế enzyme α-amylase và α-glucosidase cao nhất với giá trị IC50 lần lượt là (52,95±0,28 mgL-1, 88,04± 0,05 mg L-1), tương đương chất chuẩn Acarbose (60,88± 0,48 mg L-1, 92,16± 1,67 mg L-1). Cao chiết 90% MeOH được tách bằng sắc ký cột silica gel thu được Fr.2 với hiệu quả ức chế enzyme α-amylase và α-glucosidase tốt nhất (87,49% và 93,43%) ở mức nồng độ 0,1 mg/mL. Nghiên cứu đã sàng lọc được phân đoạn Fr.2.2 (phân tách từ Fr.2 bởi sắc ký cột ODS) cho hiệu quả ức chế enzyme α-amylase (IC50=26,14 mg L-1) và α-glucosidase (IC50= 21,38 mg L-1) tối ưu nhất, cao hơn 3 và 4 lần tương ứng so với chất chuẩn Acarbose. Phân tích phổ 1H-NMR và 13C-NMR đã xác định được cấu trúc hợp chất zonarol trong phân đoạn Fr.2.2. Kết quả nghiên cứu chứng minh hợp chất zonarol là nhóm chất có tiềm năng...
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