Phạm Thị Tuyết Ngân * , Vũ Hùng Hải , Vũ Ngọc Út Huỳnh Trường Giang

* Tác giả liên hệ (pttngan@ctu.edu.vn)

Abstract

The study was conducted to determine the optimal concentration of Bacillus CM3.1 on water quality and growth of whiteleg shrimp Litopenaeus vannamei. The study was designed in two trials. Trial 1 evaluated the decomposition of the organic matter in shrimp pond effluent by using Bacillus CM3.1 at different doses (102, 103, 104, 105 and 106 CFU/mL) during 48 h. Trial 2 assessed the effects of Bacillus CM3.1 on water quality and growth of whiteleg shrimp. Shrimp were randomly distributed in 500L composite tanks with a density of 100 individuals/tank. The tanks were designed with 4 treatments including (i) control (without bacteria) and groups added Bacillus CM3.1 into rearing water to reach final doses of (ii) 102, (iii) 103, and (iv) 104 CFU/mL. Each group was set in 3 replicates and lasted in 60 days. The results showed that addition of Bacillus at different doses accelerated organic decomposition of shrimp effluent resulting in an increasing concentration of TAN, and simultaneously a significant decrease in COD, TSS and OSS concentration after 48h. Administration of Bacillus CM3.1 into rearing water at a dose of 104 CFU/mL improved significantly water quality parameters such as TAN, N-NO2-, BOD5, COD and total Bacillus count. A significant decrease in total Vibrio count was recorded in groups added bacteria. Growth performance parameters of shrimp including specific growth rate (SGR), survival rate and biomass significantly increased in all groups added Bacillus, especially at a dose of 104 CFU/mL.

Keywords: Bacillus CM3.1, water quality, growth performance, Litopenaeus vannamei

Tóm tắt

Nghiên cứu được thực hiện nhằm xác định mật độ tối ưu của Bacillus CM3.1 lên chất lượng nước và tăng trưởng của tôm thẻ chân trắng Litopenaeus vannamei. Nghiên cứu bao gồm 2 thí nghiệm. Thí nghiệm 1 đánh giá khả năng phân hủy vật chất hữu cơ của chủng Bacillus CM3.1 ở các mật độ khác nhau (102, 103, 104, 105 và 106 CFU/mL) trong 48 giờ. Thí nghiệm 2 đánh giá ảnh hưởng của Bacillus CM3.1 lên chất lượng nước và tăng trưởng của tôm. Tôm được bố trí ngẫu nhiên vào bể composite 500L  với mật độ 100 con/bể 4 nghiệm thức (đối chứng và 3 nghiệm thức bổ sung với mật độ Bacillus 102, 103, 104 CFU/mL), mỗi nghiệm thức được lặp lại 3 lần và thời gian nuôi 60 ngày. Kết quả cho thấy việc bổ sung Bacillus CM3.1 ở các nồng độ khác nhau thúc đẩy quá trình phân hủy vật chất hữu cơ dẫn đến gia tăng hàm lượng TAN trong nước thải, đồng thời giảm đáng kể hàm lượng COD, TSS và OSS sau 48 giờ. Khi bổ sung chủng Bacillus CM3.1 vào nước ương tôm ở mật độ 104 CFU/mL giúp cải thiện đáng kể các thông số TAN, N-NO2-, BOD5, COD và mật độ Bacillus. Mật độ tổng Vibrio trong nước giảm đáng kể ở các nghiệm thức bổ sung vi khuẩn. Các thông số tăng trưởng như tốc độ tăng trưởng tương đối về khối lượng (SGR), tỉ lệ sống và sinh khối tôm tăng đáng kể ở các nghiệm thức bổ sung Bacillus, đặc biệt là nghiệm thức 104 CFU/mL.

Từ khóa: Bacillus CM3.1, chất lượng nước, tăng trưởng, Litopenaeus vannamei.

Article Details

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