Isolation and determination of the starch-degrading and E. coli-resistant characteristics of Bacillus spp. isolated from bread wastewater
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Abstract
Environmental pollution from food production facilities is becoming increasingly common. Therefore, research on Bacillus strains capable of producing amylase enzymes to treat wastewater rich in starch, such as wastewater from bread production, has become an urgent issue. From wastewater sources in Can Tho, 20 Bacillus spp. strains have been isolated with the ability to hydrolyze starch, showing clearing zone diameters ranging from 9 mm to 30 mm for 2 to 8 days. The strain NK16 exhibited the highest starch hydrolysis capability, producing 1.798 mg/mL of reducing sugars after 6 days. The results of the antibacterial capability survey showed that NK16 isolate had the highest antibacterial activity against Escherichia coli, with an inhibition zone diameter of 9.33 mm. Using 16S rRNA gene sequencing combined with several biochemical tests, NK16 was identified as Bacillus sp. and Bacillus velezensis with 99.73% similarity.
Tóm tắt
Ô nhiễm môi trường do các cơ sở sản xuất thực phẩm gây ra đang trở nên ngày càng phổ biến. Do đó, việc nghiên cứu, tìm kiếm các chủng vi khuẩn Bacillus có khả năng sản xuất enzyme amylase để xử lý nước thải ô nhiễm chứa nhiều tinh bột như nước thải từ sản xuất bánh mì đang là vấn đề cấp bách. Từ nguồn nước thải tại Cần Thơ, 20 chủng Bacillus spp. đã được phân lập với khả năng phân giải tinh bột, đường kính vòng phân giải dao động từ 9 mm đến 30 mm trong khoảng thời gian từ 2 đến 8 ngày khảo sát. Chủng vi khuẩn NK16 có khả năng phân giải tinh bột, tạo ra hàm lượng đường khử cao nhất đạt 1,798 mg/mL sau thời gian khảo sát 6 ngày. Chủng vi khuẩn NK16 cũng có khả năng kháng Escherichia coli mạnh nhất với đường kính vòng kháng khuẩn là 9,33 mm. Bằng phương pháp giải trình tự gene 16S rRNA kết hợp một số thử nghiệm sinh hóa đã xác định được rằng chủng vi khuẩn NK16 là thuộc chi Bacillus sp. và có độ tương đồng lên đến 99,73% với Bacillus velenzensis.
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