Ảnh hưởng của các yếu tố dinh dưỡng và môi trường nuôi cấy đến khả năng hòa tan lân của Enterobacter hormaechei GC1.3 phân lập từ đất rễ lúa hoang Gò Công – Đồng Tháp
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Abstract
This study evaluated the effects of nutritional and environmental culture conditions on the phosphate-solubilizing ability of Enterobacter hormaechei GC1.3 isolated from the rhizosphere soil of wild rice in Go Cong, Dong Thap Province, Vietnam. The strain was cultivated in NBRIP medium, and the effects of inorganic phosphate concentration, carbon sources, nitrogen sources, carbon-nitrogen combinations, insoluble phosphate forms (FePO₄ and AlPO₄), temperature, and cultivation conditions were investigated. The results showed that strain GC1.3 exhibited the highest phosphate-solubilizing activity in a medium containing 5 g/L Ca₃(PO₄)₂, 10 g/L glucose, and 0.1 g/L (NH₄)₂SO₄. The combination of glucose and (NH₄)₂SO₄ was identified as the optimal condition, yielding a maximum soluble phosphate concentration of 800.07 mg/L. The strain solubilized FePO₄ more effectively than AlPO₄, with the highest soluble phosphate concentration reaching 142.96 mg/L. Shaking cultivation at 25-30°C resulted in the greatest phosphate-solubilizing efficiency. These findings demonstrate the potential of E. hormaechei GC1.3 as a microbial inoculant for enhancing phosphorus availability and uptake in rice cultivation, reducing chemical fertilizer inputs, and supporting sustainable agricultural production.
Tóm tắt
Nghiên cứu được thực hiện nhằm đánh giá ảnh hưởng của các yếu tố dinh dưỡng và điều kiện nuôi cấy đến khả năng hòa tan lân của chủng Enterobacter hormaechei GC1.3 phân lập từ đất vùng rễ lúa hoang tại Gò Công, Đồng Tháp. Chủng vi khuẩn được nuôi cấy trên môi trường NBRIP và khảo sát ảnh hưởng của nồng độ phosphate vô cơ, nguồn carbon, nguồn nitrogen, sự kết hợp carbon-nitrogen, các dạng phosphate khó tan (FePO₄ và AlPO₄), nhiệt độ và điều kiện nuôi cấy. Kết quả cho thấy chủng GC1.3 đạt hiệu quả hòa tan lân cao nhất trong môi trường chứa 5 g/L Ca₃(PO₄)₂, 10 g/L glucose và 0,1 g/L (NH₄)₂SO₄. Tổ hợp glucose và (NH₄)₂SO₄ là điều kiện tối ưu cho hàm lượng lân hòa tan đạt 800,07 mg/L. Chủng có khả năng hòa tan FePO₄ hiệu quả hơn AlPO₄, với hàm lượng lân hòa tan cao nhất đạt 142,96 mg/L. Điều kiện nuôi lắc ở 25 - 30°C cho hiệu quả hòa tan lân cao nhất. Kết quả nghiên cứu cho thấy E. hormaechei GC1.3 có tiềm năng ứng dụng trong sản xuất chế phẩm vi sinh nhằm tăng khả năng hấp thu lân của cây lúa, giảm sử dụng phân bón hóa học và góp phần phát triển nông nghiệp bền vững.
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Tài liệu tham khảo
Agboola, A. A., Ogunnusi, T. A., Dayo-Olagbende, O. G., & Akpor, O. B. (2023). Optimization of phosphate solubilization activity of Enterobacter cloacae and Enterobacter hormaechei from rhizosphere. The Open Microbiology Journal, 17. e187428582308080. https://doi.org/10.2174/18742858-v17-230823-2023-8.
Akanmu, A. O., Olowe, O. M., Phiri, A. T., Nirere, D., Odebode, A. J., Umuhoza, N. J. K., Asemoloye, M. D. & Babalola, O.O. (2023). Bioresources in organic farming: Implications for sustainable agricultural systems. Horticulturae, 9(6), 659. https://doi.org/10.3390/horticulturae9060659.
Alemneh, A. A., Cawthray, G. R., Zhou, Y., Ryder, M. H., & Denton, M. D. (2025). A new isolation methodology for phosphate-solubilizing bacteria using a step-wise enrichment process. Journal of Applied Microbiology, 136(5), lxaf101. https://doi.org/10.1093/jambio/lxaf101.
An, R., & Moe, L. A. (2016). Regulation of pyrroloquinoline quinone-dependent glucose dehydrogenase activity in the model rhizosphere dwelling bacterium Pseudomonas putida KT2440. Applied and Environmental Microbiology, 82(16), 4955-4964. https://doi.org/10.1128/AEM.01211-16.
Anderson, H., Johengen, T., Miller, R. J., & Godwin, C. (2021). Accelerated sediment phosphorus release in Lake Erie's central basin during seasonal anoxia. Limnology and Oceanography, 67(2), 350-362. https://doi.org/10.1002/lno.11900.
Bertani, G. (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. Journal of Bacteriology, 62(3), 293-300. https://doi.org/10.1128/jb.62.3.293-300.1951
Bharwad, K., & Rajkumar, S. (2020). Modulation of PQQ-dependent glucose dehydrogenase (mGDH and sGDH) activity by succinate in phosphate solubilizing plant growth promoting Acinetobacter sp. SK2. 3 Biotech, 10(1), 5. https://doi.org/10.1007/s13205-019-1991-2.
Bi, Y., Xiao, L., & Liu, R. (2019). Response of AMF and PSB in coal mine soil remediation. International Journal of Coal Science & Technology, 6(4), 603-610. https://doi.org/10.1007/s40789-019-00270-7.
Bilal, S., Hazafa, A., Ashraf, I., Alamri, S., Siddiqui, M. H., Ramzan, A., Qamar, N., Sher, F., & Naeem, M. (2021). Comparative effect of phosphous-solubilizing bacteria and phosphous fertilizer on mung bean yield. Plants, 10(10), 2079. https://doi.org/10.3390/plants10102079.
Chouyia, F. E., Ventorino, V., & Pepe, O. (2022). Diversity, mechanisms and beneficial features of phosphate-solubilizing Streptomyces in sustainable agriculture: A review. Frontiers in Plant Science, 13, 1035358. https://doi.org/10.3389/fpls.2022.1035358.
Elhaissoufi, W., Ghoulam, C., Barakat, A., Zeroual, Y., & Bargaz, A. (2022). Phosphate bacterial solubilization and crop productivity. Journal of Advanced Research, 38, 13-28. https://doi.org/10.1016/j.jare.2021.08.014.
Habte, M., & Osorio, N. W. (2012). Effect of nitrogen form on the effectiveness of a phosphatesolubilizing fungus to dissolve rock phosphate. Journal of Biofertilizers & Biopesticides, 3(5), 127. http://dx.doi.org/10.4172/2155-6202.1000127.
Han, Y., Wang, C., Li, X., Cao, X., Cao, A., & Zhao, N. (2014). Isolation and identification of saline tolerance phosphate-solubilizing bacteria derived from salt-affected soils and their mechanisms of P-solubilizing. Lecture Notes in Electrical Engineering, 293, 1259-1266. https://doi.org/10.1007/978-3-642-37922-2_135.
Hương, N. T., Hà, T. T. T., & Giang, N. V. (2018). Phân lập, tuyển chọn vi khuẩn có khả năng phân giải phosphate khó tan từ đất vùng rễ lúa ở tỉnh Hải Dương. Tạp chí Khoa học Nông nghiệp Việt Nam, 60(8), 18-22.
Jain, C., Rodriguez-R, L. M., Phillippy, A. M., Konstantinidis, K. T., & Aluru, S. (2018). High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nature Communications, 9, 5114. https://doi.org/10.1038/s41467-018-07641-9.
John, A. G., Reshma, M. R., Beena, V. I., Santhosh, C. & Vijayan, M.S. (2024). Transformation of phosphorus fractions in various soil types of Kerala: An incubation study. Journal of Scientific Research and Reports, 30(7), 529-542. https://doi.org/10.9734/jsrr/2024/v30i72167.
Lambers, H. (2022). Phosphous acquisition and utilization in plants. Annual Review of Plant Biology, 73, 17-42. https://doi.org/10.1146/annurev-arplant-102720-125738.
Malusá, E., Sas-Paszt, L., & Ciesielska, J. (2012). Technologies for beneficial microorganisms inocula used as biofertilizers. The Scientific World Journal, 2012, 491206. https://doi.org/10.1100/2012/491206.
Mardad, I., Serrano, A., & Soukri, A. (2014). Effect of carbon, nitrogen sources and abiotic stress on phosphate solubilization by bacterial strains isolated from a Moroccan rock phosphate deposit. Journal of Advanced Chemical Engineering, 4(1), 102. https://doi.org/10.4172/2090-4568.1000102.
Mendoza-Arroyo, G. E., Chan-Bacab, M. J., Aguila-Ramírez, R. N., Ortega-Morales, B. O., Canché Solís, R. E., Chab-Ruiz, A. O., Cob-Rivera, K. I., Dzib-Castillo, B., Tun-Che, R. E. & Camacho-Chab, J. C. (2020). Inorganic phosphate solubilization by a novel isolated bacterial strain Enterobacter sp. Itcb-09 and its application potential as biofertilizer. Agriculture, 10(9), 383. https://doi.org/10.3390/agriculture10090383.
Mosimann, C., Oberhänsli, T., Ziegler, D., Nassal, D., Kandeler, E., Boller, T., Mäder, P., Thonar, C. (2017). Tracing Pseudomonas strains in maize rhizosphere. Frontiers in Microbiology, 7, 2150. https://doi.org/10.3389/fmicb.2016.02150.
Murphy, J., & Riley, J. P. (1962). A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta, 27, 31-36.
https://doi.org/10.1016/S0003-2670(00)88444-5.
Nautiyal, C. S. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, 170(1), 265-270. https://doi.org/10.1016/S0378-1097(98)00555-2.
Nguyen, N., Rabiet, M., Grybos, M., & Deluchat, V. (2023). Does anoxia promote the mobilization of P-bearing colloids from dam reservoir sediment?. Water Research, 245, 120568. https://doi.org/10.1016/j.watres.2023.120568
Pan, L., & Cai, B. (2023). Phosphate-solubilizing bacteria: Advances in their physiology, molecular mechanisms and microbial community effects. Microorganisms, 11(12), 2904. https://doi.org/10.3390/microorganisms11122904.
Pan, M., O’Flaherty, S., Hibberd, A., Gerdes, S., Morovic, W., & Barrangou, R. (2025). The curated Lactobacillus acidophilus NCFM genome provides insights into strain specificity and microevolution. BMC Genomics, 26(1), 11177. https://doi.org/10.1186/s12864-024-11177-2.
Qingwei, Z., Lushi, T., Yu, Z., Yu, S., Wanting, W., Jiangchuan, W., Xiaolei, D., Xuejiao, H., & Bilal, M. (2023). Isolation and characterization of phosphate-solubilizing bacteria from rhizosphere of poplar on road verge and their antagonistic potential against various phytopathogens. BMC Microbiology, 23, 221. https://doi.org/10.1186/s12866-023-02953-3.
Raniro, H. R., Hernandez-Mora, A., Duboc, O., Staudinger, C., & Santner, J. (2025). Phosphorus release dynamics from cover crop residues: A comparison between single species and mixtures. Soil Use and Management, 41, e70082. https://doi.org/10.1111/sum.70082.
Rawal, N., Pande, K. R., Shrestha, R., & Vista, S. (2022). Phosphorus and potassium mineralization as affected by phosphorus levels and soil types under laboratory condition. Agrosystems, Geosciences & Environment, 5, e20229.
https://doi.org/10.1002/agg2.20229.
Richardson, A. E., & Simpson, R. J. (2011). Soil microorganisms mediating phosphous availability. Plant Physiology, 156(3), 989-996. https://doi.org/10.1104/pp.111.175448.
Sanchez-Gonzalez, M. E., Mora-Herrera, M. E., Wong-Villarreal, A., De La Portilla-López, N., Sanchez-Paz, L., Lugo, J., Vaca-Paulín, R., Del Aguila, P., & Yañez-Ocampo, G. (2023). Effect of pH and carbon source on phosphate solubilization by bacterial strains in Pikovskaya medium. Microorganisms, 11(1), 49. https://doi.org/10.3390/microorganisms11010049.
Santoro, V., Schiavon, M., & Celi, L. (2024). Role of soil abiotic processes on phosphorus availability and plant responses with a focus on strigolactones in tomato plants. Plant Soil, 494, 1-49.
https://doi.org/10.1007/s11104-023-06266-2.
Sharma, S. B., Sayyed, R. Z., Trivedi, M. H. & Gobi, T. A. (2013). Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus, 2, 587. https://doi.org/10.1186/2193-1801-2-587
Sokol, N. W., Slessarev, E., Marschmann, G. L., Nicolas, A., Blazewicz, S. J., Brodie, E. L., et al. (2022). Life and death in the soil microbiome. Nature Reviews Microbiology, 20(7), 415-430. https://doi.org/10.1038/s41579-022-00695-z.
Sokolova, E. A., Mishukova, O. V., Hlistun, I. V., Tromenschleger, I. N., Chumanova, E. V., & Voronina, E. N. (2024). Phenotypic and genomic analysis of Enterobacter ludwigii strains: insights into mechanisms enhancing plant growth both under normal conditions and in response to supplementation with mineral fertilizers and exposure to stress factors. Plants, 13(24), 3551. https://doi.org/10.3390/ plants13243551.
Sokolova, E. A., Khlistun, I. V., Mishukova, O. V., Tromenschleger, I. N., Chumanova, E. V., & Voronina, E. N. (2025). Strain-specific phosphate mobilization in Enterobacter: Organic acid production and genomic architecture of solubilization mechanisms. International Journal of Molecular Sciences, 27(1), 322. https://doi.org/10.3390/ijms27010322.
Tang, Y., Wang, L., Fu, J., Zhou, F., Wei, H., Wu, X., Fan, S., Zhang, X. (2025). Unraveling the microecological mechanisms of phosphate-solubilizing Pseudomonas asiatica JP233 through metagenomics. Frontiers in Microbiology, 16, 1538117. https://doi.org/10.3389/fmicb.2025.1538117.
Thy, C. T. A., Nhung, Đ. T. Y., & Nghĩa, N. K. (2021). Khả năng hòa tan lân và đối kháng với nấm Fusarium solani của dòng nấm Aspergillus niger H4.7 ở điều kiện phòng thí nghiệm. Tạp chí Khoa học Đại học Cần Thơ, 57(5), 148-161. https://doi.org/10.22144/ctu.jvn.2021.152.
https://doi.org/10.1186/s12866-022-02715-7.
Wang, T., Xu, J., Chen, J., Liu, P., Hou, X., Yang, L., & Zhang, L. (2024). Progress in microbial fertilizer regulation of crop growth and soil remediation research. Plants, 13(3), 346. https://doi.org/10.3390/plants13030346.
Wendimu, A., Yoseph, T., & Ayalew, T. (2023). Ditching phosphatic fertilizers for phosphate-solubilizing biofertilizers: A step towards sustainable agriculture and environmental health. Sustainability, 15(2), 1713. https://doi.org/10.3390/su15021713.