Hoạt tính kháng nấm của các chủng Bacillus phân lập từ vùng rễ đậu nành (Glycine max (l.) Merrill) với Aspergillus flavus, Aspergillus tubingensis và Fusarium falciforme
,
,
và
Article Sidebar
Full Text: 
PDF
Abstract
Soybean (Glycine max L.) seed yield and quality are frequently reduced by fungal diseases. This study evaluated the antifungal activity of four bacterial strains, Bacillus velezensis AG8.2, Bacillus thuringiensis AG9.2, Bacillus siamensis AG11.2, and Bacillus subtilis AG16.2, against Aspergillus tubingensis, Aspergillus flavus, and Fusarium falciforme. In dual-culture assays, mycelial growth inhibition ranged from 6.15 to 54.3% for A. tubingensis, from 0 to 34.2% for A. flavus, and from 21.2 to 51.8% for F. falciforme. Cell-free supernatants of the tested strains inhibited both mycelial growth and spore germination, with stronger effects observed during the spore germination stage. In addition, volatile organic compounds produced by the bacterial strains exhibited considerable antifungal activity, with greater efficacy against F. falciforme than against the two Aspergillus species. These findings demonstrate the potential of the tested Bacillus strains for development as biocontrol agents for sustainable management of soybean fungal diseases.
Tóm tắt
Năng suất và chất lượng hạt đậu nành (Glycine max L.) thường bị suy giảm do sự tấn công của các nấm bệnh. Nghiên cứu này được thực hiện nhằm đánh giá hoạt tính kháng nấm của bốn chủng vi khuẩn Bacillus velezensis AG8.2, Bacillus thuringiensis AG9.2, Bacillus siamensis AG11.2 và Bacillus subtilis AG16.2 đối với Aspergillus tubingensis, Aspergillus flavus và Fusarium falciforme. Trong thử nghiệm đồng nuôi cấy, tỷ lệ ức chế sự phát triển sợi nấm dao động lần lượt từ 6,15 đến 54,3% đối với A. tubingensis, từ 0 đến 34,2% đối với A. flavus và từ 21,2 đến 51,8% đối với F. falciforme. Dịch nổi vô bào của các chủng khảo sát có khả năng ức chế cả sự phát triển sợi nấm và sự nảy mầm bào tử, đặc biệt trên giai đoạn nảy mầm của bào tử. Bên cạnh đó, các hợp chất hữu cơ dễ bay hơi cũng thể hiện hoạt tính kháng nấm đáng kể, với hiệu quả cao hơn trên F. falciforme. Các chủng Bacillus thử nghiệm cho thấy tiềm năng phát triển chế phẩm sinh học nhằm quản lý bền vững các bệnh hại trên cây đậu nành.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Tài liệu tham khảo
AboDalam, H., Ragab, M., Zayton, M. E., & Mannaa, M. (2026). Biocontrol of Aspergillus flavus in stored wheat using Bacillus sp. B3 and Bacillus sp. Mn1 with antagonistic capacity through volatile compounds production. Egyptian Journal of Biological Pest Control, 36, 7.
https://doi.org/10.1186/s41938-025-00880-1
Agha, S. I., Ullah, M., Khan, A., Jahan, N., Ullah, S. M., Tabassum, B., Parveen, S., Rehmat, Z., Hussain, A., Ahmed, S., & Hamid Hamdard, M. (2023). Biocontrol rhizobacteria enhances growth and yield of wheat (Triticum aestivum) under field conditions against Fusarium oxysporum. Bioengineered, 14(1), 2260923.
https://doi.org/10.1080/21655979.2023.2260923
Aphaiso, P., Mahakhan, P., & Sawaengkaew, J. (2024). Bacillus siamensis 3BS12-4 Extracellular Compounds as a Potential Biological Control Agent against Aspergillus flavus. Journal of microbiology and biotechnology, 34(8), 1671–1679.
https://doi.org/10.4014/jmb.2402.02053
Chaves-López, C., Serio, A., Gianotti, A., Sacchetti, G., Ndagijimana, M., Ciccarone, C., Stellarini, A., Corsetti, A., & Paparella, A. (2015). Diversity of food-borne Bacillus volatile compounds and influence on fungal growth. Journal of Applied Microbiology, 119(2), 487–499. https://doi.org/10.1111/jam.12847
Etesami, H., Jeong, B. R., & Glick, B. R. (2023). Biocontrol of plant diseases by Bacillus spp. Physiological and Molecular Plant Pathology, 126, 102048. https://doi.org/10.1016/j.pmpp.2023.102048
Food and Agriculture Organization of the United Nations (FAO). (2026). Crops and livestock products. https://www.fao.org/faostat/en/#data/QCL on February 24, 2026.
Gowtham, H. G., Hariprasad, P., Chandra Nayak, S., & Niranjana, S. R. (2016). Application of rhizobacteria antagonistic to Fusarium oxysporum f. sp. lycopersici for the management of Fusarium wilt in tomato. Rhizosphere, 2, 72-74.
http://dx.doi.org/10.1016/j.rhisph.2016.07.008
Guevara-Avendaño, E., Bravo-Castillo, K. R., Monribot-Villanueva, J. L., Kiel-Martínez, A. L., Ramírez-Vázquez, M., Guerrero-Analco, J. A., & Reverchon, F. (2020). Diffusible and volatile organic compounds produced by avocado rhizobacteria exhibit antifungal effects against Fusarium kuroshium. Brazilian Journal of Microbiology, 51(3), 861–873. https://doi.org/10.1007/s42770-020-00249-6
Hafez, M., Abdelmagid, A., Aboukhaddour, R., Adam, L. R., & Daayf, F. (2021). Fusarium Root Rot Complex in Soybean: Molecular Characterization, Trichothecene Formation, and Cross-Pathogenicity. Phytopathology, 111(12), 2287–2302.
https://doi.org/10.1094/PHYTO-03-21-0083-R
Han, J. H., Shim, H., Shin, J. H., & Kim, K. S. (2015). Antagonistic activities of Bacillus spp. strains isolated from tidal flat sediment towards anthracnose pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea. The Plant Pathology Journal, 31(2), 165-175.
https://doi.org/10.5423/PPJ.OA.03.2015.0036
Han, S., Chen, J., Zhao, Y., Cai, H., & Guo, C. (2021a). Bacillus subtilis HSY21 can reduce soybean root rot and inhibit the expression of genes related to the pathogenicity of Fusarium oxysporum. Pesticide Biochemistry and Physiology, 178, 104916. https://doi.org/10.1016/j.pestbp.2021.104916
Han, X., Shen, D., Xiong, Q., Bao, B., Zhang, W., Dai, T., Zhao, Y., Borriss, R., & Fan, B. (2021b). The Plant-Beneficial Rhizobacterium Bacillus velezensis FZB42 Controls the Soybean Pathogen Phytophthora sojae Due to Bacilysin Production. Applied and Environmental Microbiology, 87(23), e0160121. https://doi.org/10.1128/AEM.01601-21
Hirozawa, M. T., Ono, M. A., de Souza Suguiura, I. M., Bordini, J. G., Hirooka, E. Y., & Ono, E. Y. S. (2024). Antifungal effect and some properties of cell-free supernatants of two Bacillus subtilis isolates against Fusarium verticillioides. Brazilian Journal of Microbiology, 55(3), 2527–2538.
https://doi.org/10.1007/s42770-024-01414-x
Li, Z., He, F., Gai, X., Zhu, H., He, S., Xuan, Y. H., & Bai, L. (2025). The growth, development and infection process of the plant pathogen Fusarium. Plant signaling & behavior, 20(1), 2573097.
https://doi.org/10.1080/15592324.2025.2573097
Liên, N. T., Oanh, N. T. P., & Khoa, N. Đ. (2023). Nghiên cứu đặc điểm của Bacillus amyloliquefaciens VL4.6 tác nhân kiểm soát sinh học đối kháng với Colletotrichum lagenarium gây bệnh thán thư trên dưa leo. Tạp chí Nông nghiệp và Phát triển Nông thôn, 10, 34-41.
Liu, Y., Wei, X., Chang, F., Yu, N., Guo, C., & Cai, H. (2023). Distribution and Pathogenicity of Fusarium Species Associated with Soybean Root Rot in Northeast China. The Plant Pathology Journal, 39(6), 575–583. https://doi.org/10.5423/PPJ.OA.06.2023.0086
Moreira, R. F., Pires, E. B. E., Sousa, O. F., Alves, G. B., Viteri Jumbo, L. O., Santos, G. R., Maia, L. J., Ribeiro, B. M., Smagghe, G., Perino, E. H. B., Hausmann, R., Oliveira, E. E., & Aguiar, R. W. S. (2025). A Novel Neotropical Bacillus siamensis Strain Inhibits Soil-Borne Plant Pathogens and Promotes Soybean Growth. Microorganisms, 13(6), 1366. https://doi.org/10.3390/microorganisms13061366
Olszak-Przybyś, H., Przybyś, M., Bojarszczuk, J., & Księżak, J. (2026). Characterization of Fungi Communities in Organic Soybean Seeds Using DNA Sequencing: Effects of Cultivar and Location. Pathogens (Basel, Switzerland), 15(2), 239.
https://doi.org/10.3390/pathogens15020239.
Pagano, M. C., & Miransari, M. (2016). 1-The importance of soybean production worldwide. Abiotic and biotic stresses in soybean production. In: M. Miransari (Eds), Abiotic and Biotic Stresses in Soybean Production Soybean Production: Volume 1 (pp. 1–26). Academic Press.
https://doi.org/10.1016/B978-0-12-801536-0.00001-3
Shafi, J., Tian, H., & Ji, M. (2017). Bacillus species as versatile weapons for plant pathogens: a review. Biotechnology and Biotechnological Equipment, 31(3), 446–459. https://doi.org/10.1080/13102818.2017.1286950
Sun, L., Wang, W., Zhang, X., Gao, Z., Cai, S., Wang, S., & Li, Y. (2023). Bacillus velezensis BVE7 as a promising agent for biocontrol of soybean root rot caused by Fusarium oxysporum. Frontiers in Microbiology, 14, 1275986. https://doi.org/10.3389/fmicb.2023.1275986
Trinh, L. L., Nguyen, N. M. D, & Nguyen, H. H. (2024). Cell-free supernatant crude extracts of mold-competing bacteria protect peanut crops against yellow mold disease caused by Aspergillus flavus AF1. Biocatalysis and agricultural biotechnology, 56, 103028. https://doi.org/10.1016/j.bcab.2024.103028
Tudi, M., Daniel Ruan, H., Wang, L., Lyu, J., Sadler, R., Connell, D., Chu, C., & Phung, D. T. (2021). Agriculture Development, Pesticide Application and Its Impact on the Environment. International Journal of Environmental Research and Public Health, 18(3), 1112. https://doi.org/10.3390/ijerph18031112
Van, A. (2025). Microbial Volatile Organic Compound: Biochemical Signatures and Applications. Journal of microbial and biochemical technology, 17, 635.
Wang, Y., Yuan, J., Zhao, R., Yuan, S., Su, Y., Jiao, W., Huo, X., Wang, M., Fan, W., & Wang, C. (2025). Biocontrol Activity of Volatile Organic Compounds Emitted from Bacillus paralicheniformis 2-12 Against Fusarium oxysporum Associated with Astragalus membranaceus Root Rot. Microorganisms, 13(8), 1782.
https://doi.org/10.3390/microorganisms13081782
Wu, L., Hwang, S. F., Strelkov, S. E., Fredua-Agyeman, R., Oh, S. H., Bélanger, R. R., Wally, O., & Kim, Y. M. (2024). Pathogenicity, Host Resistance, and Genetic Diversity of Fusarium Species under Controlled Conditions from Soybean in Canada. Journal of Fungi, 10(5), 303.
https://doi.org/10.3390/jof10050303
Yuan, S., Wu, Y., Jin, J., Tong, S., Zhang, L., & Cai, Y. (2023). Biocontrol Capabilities of Bacillus subtilis E11 against Aspergillus flavus In Vitro and for Dried Red Chili (Capsicum annuum L.). Toxins, 15(5), 308. https://doi.org/10.3390/toxins15050308.
Zhang, C., Liu, Z., Yang, Y., Ma, Q., Zheng, Y., Xu, C., Gao, X., Gao, W., Huang, Z., & Liu, X. (2024a). Characterization of Fusarium species causing soybean root rot in Heilongjiang, China, and mechanism underlying the differences in sensitivity to DMI fungicides. Pesticide Biochemistry and Physiology, 200, 105828. https://doi.org/10.1016/j.pestbp.2024.105828
Zhang, Y., Li, Z., Wei, S., Xu, C., Chen, M., Sang, J., Han, Y., Yan, H., Li, Z., Cui, Z., & Ye, X. (2024b). Antifungal Activity and Mechanisms of 2-Ethylhexanol, a Volatile Organic Compound Produced by Stenotrophomonas sp. NAU1697, against Fusarium oxysporum f. sp. cucumerinum. Journal of Agricultural and Food Chemistry, 72(27), 15213–15227. https://doi.org/10.1021/acs.jafc.3c09851
Zhu, Q., Ma, Y., Zhang, T., Liu, W., Zhang, S., Chen, Y., Peng, D., & Zhang, X. (2025). Biocontrol Potential of Rhizosphere Bacteria Against Fusarium Root Rot in Cowpea: Suppression of Mycelial Growth and Conidial Germination. Biology, 14(8), 921. https://doi.org/10.3390/biology1408092