HIỆN TRẠNG KHÁNG THUỐC KHÁNG SINH TRÊN VI KHUẨN EDWARDSIELLA ICTALURI GÂY BỆNH GAN, THẬN MỦ TRÊN CÁ TRA (PANGASIANODON HYPOPHTHALMUS) Ở ĐỒNG BẰNG SÔNG CỬU LONG

Từ Thanh Dung * , Freddy Haesebrouck , Partrick Sorgeloos , Margo Baele , Annemie Decostere và Nguyễn Anh Tuấn

* Tác giả liên hệ (ttdung@ctu.edu.vn)

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Ngày nhận bài: 20-04-2021
Ngày duyệt đăng: 03-06-2022
Ngày xuất bản: 01-05-2010
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Dung, T. T., Haesebrouck, F. ., Sorgeloos, P. ., Baele, M. ., Decostere, A. ., & Tuấn, N. A. (2010). HIỆN TRẠNG KHÁNG THUỐC KHÁNG SINH TRÊN VI KHUẨN EDWARDSIELLA ICTALURI GÂY BỆNH GAN, THẬN MỦ TRÊN CÁ TRA (PANGASIANODON HYPOPHTHALMUS) Ở ĐỒNG BẰNG SÔNG CỬU LONG. Tạp chí Khoa học Đại học cần Thơ, (15a), 162-171. Truy vấn từ https://ctujsvn.ctu.edu.vn/index.php/ctujsvn/article/view/793
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Số. 15a (2010)
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Công nghệ

Abstract

The purpose of this study was to assess the in vitro susceptibility of 64 Vietnamese isolates of Edwardsiella ictaluri, the causal agent of the infectious disease Bacillus Necrosis Pangasius (BNP) in Pangasianodon hypophthalmus, using the agar dilution technique. None of the isolates displayed acquired resistance to amoxicillin, amoxicillin-clavulanic acid, chloramphenicol, florfenicol, gentamicin, kanamycin, neomycin and nitrofurantoin. Acquired resistance to streptomycin was detected in 83%, to oxytetracycline in 81% and to trimethoprim in 71% of the isolates. The MICs of enrofloxacin displayed indicating reduced susceptibility of a minority of isolates (3 out of the 64). For the quinolone antimicrobial agents flumequin and oxolinic acid, acquired resistance was encountered in 8 and 6% of the strains, respectively. All strains were intrinsically resistant to the polypeptide antimicrobial agent colistin. Resistance phenotypes of the E. ictaluri isolates were shown highly (73%) acquired resistance to at least three antimicrobial agents. The results of this study emphasize the strict need to control both the prophylactic and curative use of antimicrobial agents in Vietnamese aquaculture.
Keywords: Edwardsiella ictaluri, Pangasianodon hypophthalmus, theMekongDelta

Tóm tắt

Mục tiêu của nghiên cứu là đánh giá sự kháng thuốc kháng sinh trên 64 chủng vi khuẩn E. ictaluri, là tác nhân gây bệnh gan, thận mủ (BNP) trên cá tra Pangasianodon hypophthalmus. Nghiên cứu này đã sử dụng phương pháp pha loãng thuốc trên môi trường thạch. Kết quả không tìm thấy sự kháng thuốc của vi khuẩn với thuốc kháng sinh amoxicillin, amoxicillin-clavulanic acid, chloramphenicol, florfenicol, gentamicin, kanamycin, neomycin và nitrofurantoin. Tuy nhiên, đa số vi khuẩn E. ictaluri đã kháng với streptomycin (83% chủng vi khuẩn), oxytetracycline (81%), trimethoprim (71%) và kháng với nhóm quinolone như: flumequin (8%), oxolinic acid (6%) và enrofloxacin (5%). Trong khi đó, hầu hết vi khuẩn đã kháng thuốc tự nhiên đối với khánh sinh colistin (polypeptide). Đặc biệt, kết quả đã xác định trên 73 % chủng vi khuẩn E. ictaluri biểu hiện sự đa kháng thuốc (kháng ít nhất 3 loại thuốc). Kết quả nghiên cứu đã cảnh báo sự cần thiết kiểm soát nghiêm ngặt việc dùng thuốc kháng sinh để phòng trị bệnh trong nuôi trồng thủy sản ở Việt Nam.
Từ khóa: Kháng thuốc kháng sinh, Edwardsiella ictaluri, Pangasianodon hypophthalmus, Đồng bằng sông Cửu Long

Article Details

Tài liệu tham khảo

Ahne, W., Winton, J.R. and Kimura, T. 1989. Prevention of infectious diseases in aquaculture. J. Vet.Med. B.36: 561-567

Akinbowale, O.L., H. Peng, P. Grant, and M.D. Barton.2007. Antibiotic and heavy metal resistance in motile Aeromonadsand Pseudomonadsfrom rainbow trout (Oncorhynchus mykiss) farms in Australia. Int. J. Antimicrob. Agents 30:177-182.

Alderman, D.J., and T.S. Hastings. 1998. Antibiotic use in aquaculture: development of antibiotic resistance-potential for consumer health risks. Int. J. Food Sci. Tech. 33:139-155.

Aoki, T. 1988. Drug resistance plasmids from fish pathogens. Microbiol. Sci.5:219-223.

Aoki, T., and A.Takahashi. 1987. Class D tetracycline resistance determinants of R plasmids from the fish pathogens Aeromonas hydrophila, Edwardsiella tarda, andPasteurella piscicida. Antimicrob. Agents and Chemother. 31: 1278-1280.

Baele, M., P., Baele, M.Vaneechoutte, V. Storms, P.Butaye, L.A. Devriese, G. Verschraegen, M. Gillis, and F. Haesebrouck. 2000. Application of tDNA-PCR for the identification of Enterococcusspecies. J. Clin. Microbiol. 38:4201-4207.

Barnes, A.C., C.S. Lewin, T.S. Hastings, and S.G.B. Amyes. 1991. Fluorquinolones display rapid bactericidal activity and low mutation frequencies against Aeromonas salmonicida. J. Fish Dis. 14:661-667.

Cabello, F.C. 2004. Antibiotics and aquaculture. An analysis of their potential impact upon the environment, human and animal health in Chile. Fundacion Terram. Analisis de Politicas Publicas.17:11–16.

Clinical and Laboratory Standards Institute (CLSI). 2006. Methods for broth dilution susceptibility testing of bacteria isolated from aquatic animals; informational supplement, M49-A, Clinical and Laboratory Standards Institute, Wayne, USA.

Clinical and Laboratory Standards Institute (CLSI). 2008. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard, third edition, M31-A3, Clinical and Laboratory Standards Institute, Wayne, USA.

DePaola, A., J. T. Peeler and G.E. Rodrick. 1995. Effect of oxytetracycline –medicated feed on antibiotic resistance of gram-negative bacteria in catfish ponds. Appl. Environ. Microbiol.61:2335-2340.

DePaola, A., P.A. Flynn, R.M. McPhearson, and S.W. Levy. 1988. Phenotypic and genotypic characterization of tetracycline- and oxytetracycline-resistant Aeromonas hydrophilafrom cultured channel catfish (Ictalurus punctatus) and their environments. Appl. Environ. Microbiol.54:1861–1863.

Dowling, P.M. 2006. Chloramphenicol, thiamphenicol and florfenicol. In: S. Giguère, J. F. Prescott, D. Baggot, R D. Walker and P.M. Dowling (ed.),Antimicrobial Therapy in Veterinary Medicine. Blackwell Publishing Ltd, Oxford, pp. 241-248.

Dung, T.T., Z. Galina, D.T.H. Oanh, Z. Jeney, and N.A. Tuan. 1997. Results of the baseline survey on fish health management in freshwater aquaculture of the Mekong Delta, Vietnam. WEST-EAST-SOUTH (WES) NewsletterNo.6.

Dung T.T., M.Crumlish, N.T.N.Ngọc, N.Q.Thịnh và Đ.T.M.Thy. 2004. Xác định vi khuẩn gây bệnh mủ gan trên cá tra (Pangasius hypophthalmus). Tạp chí Khoa học Đại học Cần Thơ: 137-142

European Medicines Agency. Veteritary Medicines and Inspections (EMEA). 2006. Reflection paper on the use of fluoroquinolones in food-producing animals in the European Union: Development of resistance and impact on human and animal health, 23 pp.

FAO Globefish 2007. Pangasius market Report May, 2007.

http://w.w.w.clfish.com/index.php?act=changepage&code=new&id=33

Ferguson, H.W., J.F. Turnbull, A. Shinn, K. Thompson, T.T. Dung, and M. Crumlish. 2001. Bacillary necrosis in farmed Pangasius hypophthalmus(Sauvage) from the Mekong Delta, Vietnam. J. Fish Dis.24:509-513.

Gaunt, P., R. Endris, L. Khoo, T. Leard, S. Jack, T. Santucci, T. Katz, S.V.Radecki, and R. Simmons. 2003. Preliminary assessment of the tolerance and efficacy of florfenicol against E. ictaluriadministered in feed to channel catfish. J. Aquat. Anim. Health. 15:239-247.

Hawke, J. 1979. A bacterium associated with disease of pond cultured catfish, Ictalurus punctatus. J. Fish. Res. Board. Can. 36:508-1512.

Ho, S.P., T.Y. Hsu, M.H. Chen, and W.S. Wang. 2000. Antibacterial effect of chloramphenicol, thiamphenicol and florfenicol against aquatic animal bacteria. J. Vet. Med. Sci.62:479-485.

Jun, L.J., J.B. Jeong, M.D. Huh, J.K. Chung, D.L. Choi, C.H. Lee, and H.D. Jeong. 2004. Detection of tetracycline-resistance determinants by multiplex polymerase chain reaction in Edwardsiella tardaisolated from fish farms in Korea. Aquaculture. 240:89-100.

Kim, S.R., L. Nonaka and S. Suzuki. 2004. Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiol. Letters. 237:147-156.

Le, T.X., Y. Munekage, S.I. Kato. 2005. Antibiotic resistance in bacteria from shrimp farming in mangrove areas. Sci. Total Environ.349:95-105.

McGinnis, A., P. Gaunt, T. Santucci, R. Simmons, and R. Endris. 2003. In vitroevaluation of the susceptibility of Edwardsiella ictaluri, etiological agent of enteric septicemia in channel catfish, Ictalurus punctatus(Rafinesque), to florfenicol.J. Vet. Diagn.Invest.15:576-9.

McPhearson, R.M., A. DePaola, S.R. Zywno, M.L. Motes, and A.M. Guarino. 1991. Antibiotic resistance in Gram-negative bacteria from cultured catfish and aquaculture ponds. Aquaculture.99:203–211.

Michel, C., B. Kerouault, and C. Martin. 2003. Chloramphenicol and florfenicol susceptibility of fish-pathogenic bacteria isolated in France: comparison of minimum inhibitory concentration, using recommended provisory standards for fish bacteria. J. appl. Microbiol.95:1008-1015.

Ministry of Fisheries. 2005. Vietnam Fisheries Sector Programme Support, Phase II 2006 - 2010. Project ref.104.Vietnam:803-200.

Mitchell, A.J. 1997. Fish disease summaries for the southeastern United States from 1976-1995. Aquacult. Mag.23:87-93.

MoFi. 2004. Ministry of Fisheries in Vietnam. Department of legislation - Fisheries law project. http://www.mofi.gov.vn/law/default.aspx?tabid=288&ID=578

Muyembe, T., J. Vandepitte, and J. Desmyter. 1973. Natural colistin resistance in Edwardsiella tarda. Antimicrob. Agents Chemother. 4:521-524

Phuong, N.T., D.T.H. Oanh, T.T. Dung, and L.X. Sinh. 2005. Bacterial resistance to antimicrobials use in shrimps and fish farms in the Mekong Delta, Vietnam. In Proceedings of the International Workshop on antibiotic resistance in Asian Aquaculture Environments.Chiang May, Thaïland. ISBN 88-901344-3-7

Reger, P.J., D.F. Mockler, and M.A. Miller.1993. Comparison of antimicrobial susceptibility, beta-lactamase production, plasmid analysis and serum bactericidal activity in Edwardsiella tarda, E. ictaluriand E. hoshinae. J. Med. Microbiol.39: 273-281

Reimschuessel, R., and R.A. Miller. 2006. Antimicrobial drug use in aquaculture. In: S. Giguère, J. F. Prescott, D. Baggot, R D. Walker and P.M. Dowling (ed.),Antimicrobial Therapy in Veterinary Medicine. Blackwell Publishing Ltd, Oxford, pp. 593-606.

Roberts, M.C. 2005. MiniReview: Update on acquired tetracycline resistance. FEMS Microbiol. Letters. 245:195-203

Sørum, H. 2006. Antimicrobial drug resistance in fish pathogens. In F.M. Aarestrup (ed.), Antimicrobial resistance in bacteria of animal origin. American Society for Microbiology Press, Washington, DC, USA, pp. 213–238.

Stock, I., and B. Wiedemann. 2001. Natural antimicrobial susceptibility of Edwardsiellatarda, E. ictaluriand E. hoshinae. Antimicrob. Agents Chemother. 45:2245-2255.

Sarter, S., Kha, N. H. N., Hung, L.T., Jérôme Lazard, J. & Montet, D. 2007 Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish. Food Control 18: 1391-1396

Turnidge, J., and D.L. Paterson. 2007. Setting and revising antibacterial susceptibility breakpoints. Clin. Microbiol. Reviews. 20:391-408.

Van, P.T. 2005. Current status of aquaculture veterinary drugs usage for aquaculture in Vietnam. In Proceedings of the international workshop on antibiotic resistance in Asian aquaculture environments.Chiang May, Thaïland. ISBN 88-901344-3-7.

Waltman, W. D., and E.B. Shotts. 1986. Antimicrobial susceptibility of Edwardsiellaictaluri. J. Wildlife Dis. 22:173-177.

Wang, H., J.L. Dzink-Fox, M. Chen, and S.B. Levy. 2001. Genetic characterization of highly fluoroquinolone-resistant clinical Escherichia colistrains from China: role of acrRmutations. Antimicrob. Agents Chemother.45:1515–1521.

Wrzesinsk, C., L. Crouch, P. Gaunt, D. Holifield, N. Bertrand, and R. Endris. 2005. Florfenicol residue depletion in channel catfish, Ictalurus punctatus(Rafinesque). Aquaculture. 253:309-316.