Phân lập và tuyển chọn vi khuẩn có khả năng chuyển hóa glucose thành acid gluconic
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Abstract
Gluconic acid is applied in construction, food technology, pharmaceutical products, etc. Many studies indicated that microorganisms were potential candidates for the production of gluconic acid from glucose. This study aimed at the isolation of indigenous bacteria that can transform glucose to gluconic acid. Twenty-five bacterial strains capable of utilizing glucose were isolated from different fruit peels. Three strains GAB3, GBN6, and GSF5 were able to effectively convert glucose (100g/L) to gluconic acid in a minimal medium with pH ranging from 5 to 8 after 5 days of inoculation. Strain GSF5 producing 29.19 g/L showed the highest gluconic acid synthesis ability in the medium at pH6 and was identified as Staphylococcus sp. GSF5 based on 16S-rRNA gene sequence analysis and alignment.
Tóm tắt
Acid gluconic được ứng dụng trong xây dựng, công nghệ thực phẩm, dược phẩm, v.v. Một số loài vi sinh vật cũng được chứng minh có khả năng sử dụng glucose để tổng hợp acid gluconic. Nghiên cứu này được thực hiện nhằm phân lập các dòng vi khuẩn bản địa có khả năng chuyển hóa glucose thành acid gluconic. Hai mươi lăm dòng vi khuẩn có khả năng sử dụng glucose đã được phân lập từ các loại vỏ trái cây, trong đó, ba dòng GAB3, GBN6 và GSF5 chuyển hóa glucose (100 g/L) thành acid gluconic hiệu quả nhất trong môi trường khoáng tối thiểu có pH từ 5 đến 8 sau 5 ngày nuôi cấy. Dòng vi khuẩn GSF5 tổng hợp acid gluconic cao nhất, đạt 29,19 g/L khi được nuôi cấy trong môi trường có pH = 6. Kết quả phân tích và so sánh trình tự gen 16S-rRNA cho thấy dòng vi khuẩn GSF5 thuộc chi Staphylococcus và được định danh là Staphylococcus sp. GSF5.
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