Trần Thị Thanh Khương * , Lâm Phước Thành , Nguyễn Thị Kim Khang , Nguyễn Trọng Ngữ Dương Nguyễn Duy Tuyền

* Tác giả liên hệ (tttkhuong@ctu.edu.vn)

Abstract

Cryobank or cryoconservation of animal genetic resources is the collection and deep-freezing of mamalian cells. One of the important steps in the cryobank process is that the cell is isolated from animals that have been screened for free of pathogens before kept at -196°C. Cryobank is considered one of the important solutions in the recovery of livestock herds after the epidemic, which is the rapid supply of breedings negative for pathogens. Vietnam's livestock production is currently facing epidemics in livestock and poultry. Consequently, there is an urgent demand for high-yield, disease-free breedings. Cryobank along with techniques of reproductive biotechnology has been used for a very long period and frequently in industrialized countries to mass create disease-free animal breeds with valuable genetic resources and speedy responses to the market. This overview will analyze the difficulties faced by Vietnam's livestock business due to epidemics, introduce methods used globally by cryobank and reproductive biotechnologies to provide disease-free breedings, and provide comprehensive details on cryobanks for animal semen.

Keywords: Artificial insemination, breedings, cryobank, embryo transfer, re-herding

Tóm tắt

Cryobank hay cryoconservation of animal genetic resource là ngân hàng lưu trữ tế bào động vật trong điều kiện đông lạnh. Một trong những bước quan trọng trong quy trình của cryobank là nguồn tế bào được thu nhận từ vật nuôi đã được sàng lọc các mầm bệnh trước lưu trữ ở nhiệt độ -196°C. Chăn nuôi Việt Nam hiện đang đối mặt với các dịch bệnh trên đàn gia súc gia cầm nên nhu cầu về con giống sạch bệnh, có năng suất cao trở nên rất cấp thiết. Cryobank cùng với kỹ thuật công nghệ sinh học sinh sản sản xuất hàng loạt con giống sạch bệnh, đáp ứng nhanh cho thị trường đã được áp dụng rộng rãi ở các nước phát triển. Bài viết tập trung phân tích những thách thức từ dịch bệnh của ngành chăn nuôi, tổng hợp những phương pháp sản xuất con giống sạch bệnh từ cryobank và công nghệ sinh học sinh sản trên thế giới và cung cấp những quy trình cơ bản trong đông lạnh tinh trùng động vật nuôi.

Từ khóa: Cấy truyền phôi, con giống, cryobank, gieo tinh nhân tạo, tái đàn

Article Details

Tài liệu tham khảo

Agca, Y. (2012). Genome resource banking of biomedically important laboratory animals. Theriogenology, 78(8), 1653–1665. https://doi.org/10.1016/j.theriogenology.2012.08.012

Agossou, D. J., & Koluman, N. (2018). The effects of natural mating and artificial insemination using cryopreserved buck semen on reproductive performance in Alpine goats. Archives Animal Breeding, 61(4), 459–461. https://doi.org/10.5194/aab-61-459-2018

Aizpurua, J., Medrano, L., Enciso, M., Sarasa, J., Romero, A., Fernández, M. A., & Gómez-Torres, M. J. (2017). New permeable cryoprotectant-free vitrification method for native human sperm. Human Reproduction, 32(10), 2007–2015. https://doi.org/10.1093/humrep/dex281

Annandale, C. H., Smuts, M. P., Ebersohn, K., du Plessis, L., Venter, E. H., & Stout, T. A. E. (2018). Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen. Animal Reproduction Science, 195, 24–29. https://doi.org/10.1016/j.anireprosci.2018.04.080

Arraztoa, C. C., Miragaya, M. H., Chaves, M. G., Trasorras, V. L., Gambarotta, M. C., & Neild, D. M. (2017). Porcine sperm vitrification II: Spheres method. Andrologia, 49(8), e12738. https://doi.org/10.1111/and.12738

Aurich, J. E. (2012). Artificial Insemination in Horses—More than a Century of Practice and Research. Journal of Equine Veterinary Science, 32(8), 458–463. https://doi.org/10.1016/j.jevs.2012.06.011

Barbas, J. P., & Mascarenhas, R. D. (2009). Cryopreservation of domestic animal sperm cells. Cell and Tissue Banking, 10(1), 49–62. https://doi.org/10.1007/s10561-008-9081-4

Behrman, S. J., & Sawada, Y. (1966). Heterologous and Homologous Inseminations with Human Semen Frozen and Stored in a Liquid-Nitrogen Refrigerator. Fertility and Sterility, 17(4), 457–466. https://doi.org/10.1016/S0015-0282(16)36003-4

Blackburn, H. D. (2009). Genebank development for the conservation of livestock genetic resources in the United States of America. Livestock Science, 120(3), 196–203. https://doi.org/10.1016/j.livsci.2008.07.004

Blesbois, E., Seigneurin, F., Grasseau, I., Limouzin, C., Besnard, J., Gourichon, D., Coquerelle, G., Rault, P., & Tixier-Boichard, M. (2007). Semen Cryopreservation for Ex Situ Management of Genetic Diversity in Chicken: Creation of the French Avian Cryobank. Poultry Science, 86(3), 555–564. https://doi.org/10.1093/ps/86.3.555

Bratton, R. W., Foote, R. H., & Cruthers, J. C. (1955). Preliminary Fertility Results with Frozen Bovine Spermatozoa. Journal of Dairy Science, 38(1), 40–46. https://doi.org/10.3168/jds.S0022-0302(55)94935-3

Bunge, R. G., & Sherman, J. K. (1953). Fertilizing Capacity of Frozen Human Spermatozoa. Nature, 172(4382), 767–768. https://doi.org/10.1038/172767b0

Burrows, W. H., & Quinn, J. P. (1937). The Collection of Spermatozoa from the Domestic Fowl and Turkey. Poultry Science, 16(1), 19–24. https://doi.org/10.3382/ps.0160019

Bwanga, C. O., Braganca, M. M., Einarsson, S., & Rodriguez-Martinez, H. (1990). Cryopreservation of Boar Semen in Mini- and Maxi-Straws. Journal of Veterinary Medicine Series A, 37(1–10), 651–658. https://doi.org/10.1111/j.1439-0442.1990.tb00958.x

Chánh, T & Vũ, V. (2022). Khôi phục đàn heo giống hạt nhân, tạo tiền đề chăn nuôi bền vững. https://nongnghiep.vn/khoi-phuc-dan-heo-giong-hat-nhan-tao-tien-de-chan-nuoi-ben-vung-d325059.html

Critser, J. K., & Russell, R. J. (2000). Genome Resource Banking of Laboratory Animal Models. ILAR Journal, 41(4), 183–186. https://doi.org/10.1093/ilar.41.4.183

Crum, T. E., Schnabel, R. D., Decker, J. E., Regitano, L. C. A., & Taylor, J. F. (2019). CRUMBLER: A tool for the prediction of ancestry in cattle. PLOS ONE, 14(8), e0221471. https://doi.org/10.1371/journal.pone.0221471

de Sousa, R. V., da Silva Cardoso, C. R., Butzke, G., Dode, M. A. N., Rumpf, R., & Franco, M. M. (2017). Biopsy of bovine embryos produced in vivo and in vitro does not affect pregnancy rates. Theriogenology, 90, 25–31. https://doi.org/10.1016/j.theriogenology.2016.11.003

Dhama, K., Singh, R. P., Karthik, K., Chakrabort, S., Tiwari, R., Wani, M. Y., & Mohan, J. (2014). Artificial Insemination in Poultry and Possible Transmission of Infectious Pathogens: A Review. Asian Journal of Animal and Veterinary Advances, 9(4), 211–228. https://doi.org/10.3923/ajava.2014.211.228

Dương, N.X. (2022). Ngành chăn nuôi 2022: Nhận diện thách thức, tập trung giải pháp. http://nhachannuoi.vn/nganh-chan-nuoi-2022-nhan-dien-thach-thuc-tap-trung-giai-phap/

Estudillo, E., Jiménez, A., Bustamante-Nieves, P. E., Palacios-Reyes, C., Velasco, I., & López-Ornelas, A. (2021). Cryopreservation of Gametes and Embryos and Their Molecular Changes. International Journal of Molecular Sciences, 22(19), 10864. https://doi.org/10.3390/ijms221910864

FAO. (2012). Cryoconservation of Animal Genetic Resources; FAO Animal Production and Health Guidelines No. 12; FAO: Rome, Italy.

Givens, M. D. (2018). Review: Risks of disease transmission through semen in cattle. Animal, 12, s165–s171. https://doi.org/10.1017/S1751731118000708

Groeneveld, L. F., Gregusson, S., Guldbrandtsen, B., Hiemstra, S. J., Hveem, K., Kantanen, J., Lohi, H., Stroemstedt, L., & Berg, P. (2016). Domesticated Animal Biobanking: Land of Opportunity. PLOS Biology, 14(7), e1002523. https://doi.org/10.1371/journal.pbio.1002523

Holt, W. V. (2000). Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology, 53(1), 47–58. https://doi.org/10.1016/S0093-691X(99)00239-3

Iaffaldano, N., Di Iorio, M., Rusco, G., Antenucci, E., Zaniboni, L., Madeddu, M., Marelli, S., Schiavone, A., Soglia, D., Buccioni, A., Cassandro, M., Castellini, C., Marzoni, M., & Cerolini, S. (2021). Italian semen cryobank of autochthonous chicken and turkey breeds: A tool for preserving genetic biodiversity. Italian Journal of Animal Science, 20(1), 2022–2033. https://doi.org/10.1080/1828051X.2021.1993094

Jiménez-Rabadán, P., García-Álvarez, O., Vidal, A., Maroto-Morales, A., Iniesta-Cuerda, M., Ramón, M., del Olmo, E., Fernández-Santos, R., Garde, J. J., & Soler, A. J. (2015). Effects of vitrification on ram spermatozoa using free-egg yolk extenders. Cryobiology, 71(1), 85–90. https://doi.org/10.1016/j.cryobiol.2015.05.004

Jones, A. S. K., & Shikanov, A. (2020). Ovarian Tissue Cryopreservation and Novel Bioengineering Approaches for Fertility Preservation. Current Breast Cancer Reports, 12(4), 351–360. https://doi.org/10.1007/s12609-020-00390-z

Kuleshova, L., Gianaroli, L., Magli, C., Ferraretti, A., & Trounson, A. (1999). Birth following vitrification of a small number of human oocytes: Case Report. Human Reproduction, 14(12), 3077–3079. https://doi.org/10.1093/humrep/14.12.3077

Leroy, G., Boettcher, P., Besbes, B., Danchin-Burge, C., Baumung, R., & Hiemstra, S. J. (2019). Cryoconservation of Animal Genetic Resources in Europe and Two African Countries: A Gap Analysis. Diversity, 11(12), 240. https://doi.org/10.3390/d11120240

Long, J. A., Bongalhardo, D. C., Pelaéz, J., Saxena, S., Settar, P., O’Sullivan, N. P., & Fulton, J. E. (2010). Rooster semen cryopreservation: Effect of pedigree line and male age on postthaw sperm function. Poultry Science, 89(5), 966–973. https://doi.org/10.3382/ps.2009-00227

Loutradi, K. E., Kolibianakis, E. M., Venetis, C. A., Papanikolaou, E. G., Pados, G., Bontis, I., & Tarlatzis, B. C. (2008). Cryopreservation of human embryos by vitrification or slow freezing: A systematic review and meta-analysis. Fertility and Sterility, 90(1), 186–193. https://doi.org/10.1016/j.fertnstert.2007.06.010

Mahadevan, M., & Trounson, A. O. (2009). Effect of Cooling, Freezing and Thawing Rates and Storage Conditions on Preservation of Human Spermatozoa. Andrologia, 16(1), 52–60. https://doi.org/10.1111/j.1439-0272.1984.tb00234.x

Martins Pereira, E. C., Silva, A., da Costa, E. P., & Real Pereir, C. E. (2013). The Potential for Infectious Disease Contamination During the Artificial Insemination Procedure in Swine. In A. Lemma (Ed.), Success in Artificial Insemination—Quality of Semen and Diagnostics Employed. InTech. https://doi.org/10.5772/52337

Matukumalli, L. K., Lawley, C. T., Schnabel, R. D., Taylor, J. F., Allan, M. F., Heaton, M. P., O’Connell, J., Moore, S. S., Smith, T. P. L., Sonstegard, T. S., & Van Tassell, C. P. (2009). Development and Characterization of a High Density SNP Genotyping Assay for Cattle. PLoS ONE, 4(4), e5350. https://doi.org/10.1371/journal.pone.0005350

My, L. H., Phuoc, M. B. T., Tuyen, D. N. D., & Khuong, T. T. T. (2021) Đông lạnh trinh trùng lợn bằng phương pháp thủy tinh hóa. Tạp chí Nông nghiệp và Phát triển nông thôn, 7, 116-121

Neglia, G., de Nicola, D., Esposito, L., Salzano, A., D’Occhio, M. J., & Fatone, G. (2020). Reproductive management in buffalo by artificial insemination. Theriogenology, 150, 166–172. https://doi.org/10.1016/j.theriogenology.2020.01.016

Nguyên, B. (2022). Ngành chăn nuôi thiệt hại nặng vì dịch, bệnh. http://www.baodongnai.com.vn/tieu-diem/202202/nganh-chan-nuoi-thiet-hai-nang-vi-dich-benh-3104046/index.htm

Oberoi, B., Kumar, S., & Talwar, P. (2014). Study of human sperm motility post cryopreservation. Medical Journal Armed Forces India, 70(4), 349–353. https://doi.org/10.1016/j.mjafi.2014.09.006

Obuchi, T., Osada, M., Ozawa, T., Nakagawa, H., Hayashi, M., Akiyama, K., Sakagami, N., Miura, R., Geshi, M., & Ushijima, H. (2019). Comparative evaluation of the cost and efficiency of four types of sexing methods for the production of dairy female calves. Journal of Reproduction and Development, 65(4), 345–352. https://doi.org/10.1262/jrd.2019-028

Pegg, D. E. (2002). The History and Principles of Cryopreservation. Seminars in Reproductive Medicine, 20(1), 005–014. https://doi.org/10.1055/s-2002-23515

Perloff, W. H., & Steinberger, E. (1964). In vivo survival of spermatozoa in cervical mucus. American Journal of Obstetrics and Gynecology, 88(4), 439–442. https://doi.org/10.1016/0002-9378(64)90499-5

Perry, G. (2014). 2013 statistics of embryo collection and transfer in domestic farm animals. https://doi.org/10.13140/RG.2.2.22202.59842

Polge, C., Smith, A. U., & Parkes, A. S. (1949). Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures. Nature, 164(4172), 666–666. https://doi.org/10.1038/164666a0

Ponsart, C., Le Bourhis, D., Knijn, H., Fritz, S., Guyader-Joly, C., Otter, T., Lacaze, S., Charreaux, F., Schibler, L., Dupassieux, D., & Mullaart, E. (2014). Reproductive technologies and genomic selection in dairy cattle. Reproduction, Fertility and Development, 26(1), 12. https://doi.org/10.1071/RD13328

Quang, T. (2020) "Đặt hàng" doanh nghiệp cung cấp lợn giống cho người chăn nuôi. https://danviet.vn/dat-hang-doanh-nghiep-cung-cap-lon-giong-cho-nguoi-chan-nuoi-1085623.htm

Quyết định số 11/2006/QĐ-TTg, của Thủ tướng Chính phủ, ngày 12 tháng 01 năm 2006 về việc phê duyệt “Chương trình trọng điểm phát triển và ứng dụng công nghệ sinh học trong lĩnh vực nông nghiệp và phát triển nông thôn đến năm 2020”

Quyết định số 1520/2020/QD-TTg, của Thủ tướng Chính phủ, ngày 6 tháng 10 năm 2020 về việc phê duyệt “chiến lược phát triển chăn nuôi 2021-2030, tầm nhìn 2045”.

Rall, W. F., & Fahy, G. M. (1985). Ice-free cryopreservation of mouse embryos at −196 °C by vitrification. Nature, 313(6003), 573–575. https://doi.org/10.1038/313573a0

Rusco, G., Di Iorio, M., Gibertoni, P. P., Esposito, S., Penserini, M., Roncarati, A., Cerolini, S., & Iaffaldano, N. (2019). Optimization of Sperm Cryopreservation Protocol for Mediterranean Brown Trout: A Comparative Study of Non-Permeating Cryoprotectants and Thawing Rates In Vitro and In Vivo. Animals, 9(6), 304. https://doi.org/10.3390/ani9060304

Shaffner, C. S., Henderson, E. W., & Card, C. G. (1941). Viability of Spermatozoa of the Chicken Under Various Environmental Conditions. Poultry Science, 20(3), 259–265. https://doi.org/10.3382/ps.0200259

Smith, J. L., Wilson, M. L., Nilson, S. M., Rowan, T. N., Schnabel, R. D., Decker, J. E., & Seabury, C. M. (2022). Genome-wide association and genotype by environment interactions for growth traits in U.S. Red Angus cattle. BMC Genomics, 23(1), 517. https://doi.org/10.1186/s12864-022-08667-6

Soliman, F., & El-Sabrout, K. (2020). Artificial insemination in rabbits: Factors that interfere in assessing its results. Journal of Animal Behaviour and Biometeorology, 8(2), 120–130. https://doi.org/10.31893/jabb.20016

Squires, E. L., McCue, P. M., & Vanderwall, D. (1999). The current status of equine embryo transfer. Theriogenology, 51(1), 91–104. https://doi.org/10.1016/S0093-691X(98)00234-9

Thibier, M., & Wagner, H.-G. (2002). World statistics for artificial insemination in cattle. Livestock Production Science, 74(2), 203–212. https://doi.org/10.1016/S0301-6226(01)00291-3

Tomka, J., Huba, J., & Pavlík, I. (2022). State of conservation of animal genetic resources in Slovakia. Genetic Resources, 3(6), 49–63. https://doi.org/10.46265/genresj.XRHU9134

van Wagtendonk-de Leeuw, A. M. (2006). Ovum Pick Up and In Vitro Production in the bovine after use in several generations: A 2005 status. Theriogenology, 65(5), 914–925. https://doi.org/10.1016/j.theriogenology.2005.09.007

Viana, J. (2019). 2018 Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter36(4), 17.

Walters, E. M., Benson, J. D., Woods, E. J., & Critser, J. K. (2009). The history of sperm cryopreservation. In A. A. Pacey & M. J. Tomlinson (Eds.), Sperm Banking (1st ed., pp. 1–17). Cambridge University Press. https://doi.org/10.1017/CBO9781139193771.002

Wiebke, M., Hensel, B., Nitsche-Melkus, E., Jung, M., & Schulze, M. (2021). Cooled storage of semen from livestock animals (part I): Boar, bull, and stallion. Animal Reproduction Science, 106822. https://doi.org/10.1016/j.anireprosci.2021.106822

Woelders, H., Windig, J., & Hiemstra, S. (2012). How Developments in Cryobiology, Reproductive Technologies and Conservation Genomics Could Shape Gene Banking Strategies for (Farm) Animals: Technologies of Animal Gene Banking. Reproduction in Domestic Animals, 47, 264–273. https://doi.org/10.1111/j.1439-0531.2012.02085.x

Yánez-Ortiz, I., Catalán, J., Rodríguez-Gil, J. E., Miró, J., & Yeste, M. (2021). Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep. Animal Reproduction Science, 106904. https://doi.org/10.1016/j.anireprosci.2021.106904

Yang, S. (2020). Assisted reproductive technologies in nonhuman primates. In Reproductive Technologies in Animals (pp. 181–191). Elsevier. https://doi.org/10.1016/B978-0-12-817107-3.00012-6