Thành phần hóa học của cao chiết ethyl acetate từ cây ba chẽ Desmodium triangulare (Retz.) Merr
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Abstract
In this study, the chemical constituents of the ethyl acetate extract from the stem and leaves of Desmodium triangulare were investigated. The dried material sample was crushed and then extracted exhaustively with methanol to obtain a crude extract. The crude extract was completely dispersed in water and performed liquid-liquid extraction with ethyl acetate solvent to obtain ethyl acetate extract. By column chromatography on silica gel and Sephadex LH20, the separation of the ethyl acetate extract was performed and led to the isolation of four compounds. Based on 1H-NMR and 13C-NMR spectra and comparison with the previously reported data, the structures of the isolated compounds were identified as stigmasterol, methyl protocatechuate, methyl syringate, and methyl ferulate. HPLC analysis of the methanol extract was also conducted, and the results showed that the polar and non-polar compounds in this methanol extract were the main constituents.
Tóm tắt
Trong nghiên cứu này, thành phần hóa học của cao chiết ethyl acetate từ thân và lá cây ba chẽ đã được nghiên cứu. Mẫu nguyên liệu khô được nghiền nhỏ, sau đó chiết bằng phương pháp ngấm kiệt với methanol thu được cao chiết thô. Cao chiết thô được phân tán trong nước và thực hiện quá trình chiết lỏng- lỏng với dung môi ethyl acetate nhằm thu được cao ethyl acetate. Cao chiết ethyl acetate đã được phân tách bằng phương pháp sắc ký trên cột silica gel và Sephadex LH20. Kết quả đã phân lập được bốn hợp chất sạch. Dựa vào dữ liệu phổ 1H-NMR và 13C-NMR và kết hợp với các tài liệu tham khảo đã xác định được cấu trúc của bốn hợp chất hữu cơ đã phân lập là stigmasterol, methyl protocatechuate, methyl syringate và methyl ferulate. Kết quả phân tích HPLC của cao chiết methanol chỉ ra rằng các hợp chất phân cực và kém phân cực là thành phần chính của cao chiết.
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Tài liệu tham khảo
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