Huỳnh Quốc Cường , Nguyễn Việt Bách Phạm Duy Toàn *

* Người chịu trách nhiệm về bài viết: Phạm Duy Toàn (email: pdtoan@ctu.edu.vn)

Abstract

This study aimed to evaluate the ability to load polyphenol compounds from the flower extract of Wedelia trilobata L. (WT) into silk fibroin microparticles, and investigate the antioxidant activity of the products, using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) method. The WT extract was loaded into the microparticles by co-condensation method. The extract loaded fibroin microparticles possessed an average size of 7.11 µm, a high entrapment efficiency (74.10%), and an ability to control the drug release rate in the buffer pH 7.4. Moreover, the WT extract had a very high antioxidant activity (IC50 = 8.67 µg/mL) and the extract loaded microparticles also retained their antioxidant capacity (27.89%, 44.75%, and 52.61%, after 30, 90, and 180 min of incubation, respectively). Due to the ability of microparticles to sustain release the active ingredients into the dispersion medium, their ability to scavenge free radicals was time-dependent. Conclusively, the WT extract loaded fibroin microparticles is a potential application for utilizing in controlled-release formulations.

Keywords: antioxidant, microparticles, silk, Wedelia trilobata L.

Tóm tắt

Nghiên cứu được thực hiện nhằm đánh giá khả năng tải các hợp chất polyphenol từ dịch chiết hoa sài đất ba thùy (Wedelia trilobata L. - WT) vào vi hạt fibroin tơ tằm và khảo sát hoạt tính kháng oxy hóa của các sản phẩm, sử dụng phương pháp DPPH (2,2-Diphenyl-1-picrylhydrazyl). Dịch chiết được nạp vào vi hạt bằng phương pháp đồng ngưng tụ. Hệ vi hạt fibroin chứa dịch chiết có kích thước trung bình là 7,11 µm, hiệu suất tải dịch chiết khá cao (74,13%) và có khả năng kiểm soát quá trình giải phóng polyphenol trong hệ đệm pH 7,4. Hơn nữa, dịch chiết WT có hoạt tính kháng oxy hóa rất cao (IC50=8,67 µg/mL) và vi hạt sau khi được tải dịch chiết cũng giữ được khả năng kháng oxy hóa (ở các mốc thời gian 30, 90, 180 phút, lần lượt là 27,89%, 44,75%, 52,61%). Do hệ vi hạt có khả năng giải phóng hoạt chất có kiểm soát, dẫn đến khả năng kháng oxy hóa của hệ phụ thuộc vào thời gian. Tóm lại, hệ vi hạt chứa cao WT là một ứng dụng tiềm năng cho các dạng thuốc phóng thích có kiểm soát.

Từ khóa: kháng oxy hóa, Sài đất ba thùy, tơ tằm, vi hạt

Article Details

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