Nghiên cứu các điều kiện tối ưu cho sản xuất chế phẩm probiotic định hướng thay thế kháng sinh trên gia cầm từ các chủng Lactiplantibacillus
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Abstract
This study focused on optimizing the production process of probiotics from native Lactiplantibacillus for use in chicken farming, aiming to replace antibiotics. From 68 Lactiplantibacillus strains isolated from the chicken feces, five strains with the best antibacterial activity against Escherichia coli, Salmonella typhimurium FC13827 and Proteus mirabilis were selected and evaluated for safety. Four strains (L. plantarum 1582, L. plantarum JDM1, L. acidophilus NCFM, and L. agilis DSM 20509) were cultured in different media, of which the medium Mgl (containing glucose) and Mlac (containing skim milk powder) gave the best growth. Semi-industrial fermentation tests with these strains determined the optimal agitation speed to be 50 rpm, achieving a biomass of 0.6 g/L. The study provides important information on strain selection and optimization of culture conditions for native Lactiplantibacillus, aiming at reducing antibiotic use in chicken farming. However, further in vivo studies are needed to evaluate the efficacy of this product under real conditions.
Tóm tắt
Việc tối ưu hóa quy trình sản xuất chế phẩm sinh học từ Lactiplantibacillus bản địa ứng dụng trong chăn nuôi gà được nghiên cứu nhằm thay thế kháng sinh. Số lượng chủng Lactiplantibacillus được phân lập từ phân gà là 68, trong đó có 5 chủng kháng khuẩn tốt nhất chống lại vi khuẩn gây bệnh trên gà (Escherichia coli FG31-1, Salmonella typhimurium FC13827 và Proteus mirabilis MPE4069) đã được lựa chọn và đánh giá về tính an toàn. Bốn chủng (L. plantarum L3, L. plantarum L8, L. acidophilus L18, và L. agilis L27) được nuôi cấy trên các môi trường khác nhau, trong đó môi trường Mgl (chứa glucose) và Mlac (bột sữa gầy) cho sinh trưởng tốt nhất. Thử nghiệm lên men bán công nghiệp với các chủng này xác định tốc độ khuấy tối ưu là 50 vòng/phút, đạt sinh khối 0,6 g/L. Thông tin quan trọng về lựa chọn chủng và tối ưu hóa điều kiện nuôi cấy Lactiplantibacillus bản địa được cung cấp trung nghiên cứu, hướng đến hạn chế sử dụng kháng sinh trong chăn nuôi gà. Tuy nhiên, việc có thêm các nghiên cứu in vivo để đánh giá hiệu quả của sản phẩm này trong điều kiện thực tế là cần thiết.
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