Isolating Hydnophytum formicarum endophytic bacteria to generate antioxidants
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Abstract
The study aims to find the bacteria Hydnophytum formicarum endophytic bacteria that have the ability to produce antioxidants (total flavonoid content (TFC), total antioxidant capacity (TAC), and reducing power (RP)). In addition, promising bacterial strains were identified through observing morphology, and 16s rRNA. Samples of roots, stems and leaves of Hydnophytum formicarum plant in Phu Quoc city, Kien Giang province were used as raw materials to isolate bacteria. The results showed that a total of 25 endophytic bacterial strains isolated from different parts Hydnophytum formicarum had potential antioxidant activity. Among them, three bacterial strains HF-L5, HF-T11 and HF-R6 were selected for identification. The results showed that these bacterial strains belong to the Bacillus genera (strains HF-L5 and HF-T11) and Pantoea (strain HF-R6).
Tóm tắt
Nghiên cứu được thực hiện nhằm phân lập vi khuẩn nội sinh trong cây bí kỳ nam (Hydnophytum formicarum) có khả năng tạo ra chất chống oxy hoá (hàm lượng flavonoid tổng (TFC), chống oxy hoá tổng (TAC) và khả năng khử sắt (RP)). Ngoài ra, các dòng vi khuẩn có khả năng chống oxy hoá cao được định danh qua việc quan sát hình thái học,16s rRNA. Các mẫu rễ, thân và lá của cây bí kỳ nam ở thành phố Phú Quốc, tỉnh Kiên Giang được dùng làm nguyên liệu để phân lập vi khuẩn. Kết quả cho thấy tổng cộng 25 dòng vi khuẩn nội sinh được phân lập từ các bộ phận khác nhau của cây Bí kỳ nam đều có hoạt tính chống oxy hoá. Trong đó, 3 dòng vi khuẩn HF-L5, HF-T11 và HF-R6 được chọn để tiến hành định danh. Kết quả cho thấy các dòng vi khuẩn này thuộc chi Bacillus (dòng HF-L5 và HF-T11) và Pantoea (dòng HF-R6).
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