Antioxidant and hepatoprotective activities of the methanol leaf extract of Neolamarckia cadamba (Roxb.) Bosser

Phan Kim Dinh * , Dai Thi Xuan Trang and Nguyen Trong Tuan

* Corresponding author (pkdinh@ctu.edu.vn)

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Received: 20 Mar 2019
Revised: 07 May 2019
Accepted: 30 Oct 2019
Published: 30 Oct 2019
Title: Antioxidant and hepatoprotective activities of the methanol leaf extract of Neolamarckia cadamba (Roxb.) Bosser
DOI: 10.22144/ctu.jvn.2019.124
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Định, P. K., Trang, Đ. T. X., & Tuân, N. T. (2019). Antioxidant and hepatoprotective activities of the methanol leaf extract of Neolamarckia cadamba (Roxb.) Bosser. CTU Journal of Science, 55(5), 24-31. https://doi.org/10.22144/ctu.jvn.2019.124
Issue
Vol. 55 No. 5 (2019)
Section
Natural Sciences

Abstract

The chemical composition of Gao trang leaves (Neolamarckia cadamba (Roxb.) Bosser) contained alkaloids, flavonoids, glycosides, polyphenols, tannins and triterpenoids. The total polyphenol and flavonoid contents of N. cadamba were equivalent to 376.43±2.22 mg GAE/g and 613.53±90.39 mg QE/g, respectively. The antioxidant activities of the extract of  N. cadamba leaves were investigated by DPPH free radical scavenging and reducing power (RP) assays. The leaf extract possessed relatively high antioxidant activity, with EC50 value of 52.69 µg/mL and OD0.5 value of 89.68 µg/mL, which were higher than that of references as vitamin C and BHA 1.13 times and 2.97 times, respectively. The hepatoprotective activity of the leaf extract was investigated in mice which were previously treated with CCl4 to induce liver damage. The leaf extract at the dose of 100, 200 and 400 mg/kg body weight effectively reduced the level of alanine transaminase and aspartate transaminase in serum. The leaf extract also improved the oxidative stress status in mice liver through effective reduction of malondialdehyde level and increasing of glutathione level in the liver.
Keywords: Antioxidant, carbon tetrachloride (CCl4), hepatoprotection, Neolamarckia cadamba (Roxb.) Bosser

Tóm tắt

Cao lá Gáo trắng - GT (Neolamarckia cadamba (Roxb.) Bosser) có chứa các thành phần hóa học bao gồm alkaloid, flavonoid, glycoside, phenol, tannin, triterpenoid. Hàm lượng polyphenol và flavonoid được xác định lần lượt là 376,43±2,22 mg GAE/g và 613,53±90,39 mg QE/g. Hoạt tính chống oxy hóa của cao lá GT  được khảo sát bằng các phương pháp DPPH và khử sắt (RP). Kết quả cho thấy, cao lá GT có khả năng chống oxy hóa khá tốt với EC50 = 52,69 µg/mL và OD0,5 = 89,68 µg/mL, lần lượt cao hơn chất chuẩn vitamin C và BHA  là 1,13 lần và 2,97 lần. Hoạt động  bảo vệ gan của cao lá GT được khảo sát trên chuột tổn thương gan bằng CCl4 với liều 2,5 ml/kg/ngày bằng đường uống trong thời gian 4 tuần. Silymarin được sử dụng như đối chứng dương. Ở các liều khảo sát cao lá GT 100, 200, 400 mg/kg trọng lượng chuột đều cho hiệu quả làm giảm hàm lượng các enzyme ALT và AST trong huyết thanh rất tốt. Cao lá GT cũng cải thiện được trạng thái stress oxy hóa trong gan qua hiệu quả làm giảm mức MDA và làm tăng mức GSH trong mô gan.
Từ khóa: Bảo vệ gan, chống oxy hóa, Neolamarckia cadamba (Roxb.) Bosser, tetrachloric carbon (CCl4)

Article Details

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