Lê Hồng Giang *

* Tác giả liên hệ (lhgiang@ctu.edu.vn)

Abstract

Plectranthus amboinicus (Lour.) Spreng., a perennial herb, has antibacterial, anti-inflammatory, and anti-tumor effects which has been used to treat diseases such as respiratory, cardiovascular, and digestive. The study aimed to determine the effective treatment of salicylic acid (SA) to improve the accumulation of in vitro secondary metabolites in this plant. The concentrations of SA at 0, 50, 100, 150 and 200 µM were supplemented into Murashige & Skoog (1962) medium for culturing. The growth and total contents of flavonoids and phenolics in shoot samples were evaluated. The results showed that SA at 50 µM had almost no effect on the shoot growth including leaf number, root formation rate, fresh weight and chlorophyll index. However, it helped to significantly enhance the contents of these two substrates. The total flavonoid content achieved 7.08 mg QE/g dry weight and phenolics reached 2.30 mg GAE/g with the increasing of 1.14 and 1.73-fold compared to the control, respectively. Therefore, exposure to 50 µM SA could help to improve the production of in vitro secondary metabolites in this valuable medicinal plant.

Keywords: In vitro, flavonoids, phenolics, Plectranthus amboinicus, salicylic acid, secondary metabolites

Tóm tắt

Húng chanh hay tần dày lá (Plectranthus amboinicus (Lour.) Spreng. là một loài thảo dược lâu năm có tác dụng kháng khuẩn, chống viêm, kháng u được dùng để chữa các bệnh như hô hấp, tim mạch, tiêu hóa,… Nghiên cứu này nhằm xác định nồng độ salicylic acid (SA) xử lý thích hợp giúp cải thiện sự tích lũy các hợp chất biến dưỡng thứ cấp in vitro ở cây húng chanh. Các nồng độ SA ở 0, 50, 100, 150 và 200 µM được bổ sung vào môi trường nuôi cấy Murashige & Skoog (1962). Sự sinh trưởng và hàm lượng các hợp chất flavonoid và phenolic tổng số trong mẫu chồi được đánh giá. Kết quả cho thấy nồng độ SA 50 µM gần như không tác động lên sự sinh trưởng của chồi bao gồm số lá, tỷ lệ tạo rễ, khối lượng tươi và chỉ số diệp lục tố nhưng có hiệu quả tăng cường hàm lượng của cả hai nhóm hợp chất này. Hàm lượng flavonoid tổng đạt 7,08 mg QE/g khối lượng khô và phenolic đạt 2,30 mg GAE/g, lần lượt tăng gấp 1,14 và 1,73 lần so với đối chứng...

Từ khóa: Chất biến dưỡng thứ cấp, in vitro, flavonoid, phenolic Plectranthus amboinicus, salicylic acid

Article Details

Tài liệu tham khảo

Ali, M., Yu, K.W., Hahn, E.J., & Paek, K.Y. (2006).
Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and nonenzymatic antioxidant in suspension
culture Panax ginseng roots in bioreactors. Plant Cell Rep., 25(6), 613-62.

Arumugam, G., Swamy, M. K., & Sinniah, U. R. (2016). Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules, 21(369), 1-26. doi:10.3390/molecules21040369

Bipin, M., Krishna, K. R., Neha, P., Lakee S., Niraj K. G., & Shashi, P. R. (2019). Influence of salicylic acid elicitation on secondary metabolites and biomass production in in-vitro cultured Withania coagulans (L.) Dunal. Plant Archives, 19(1), 1308-1045.

Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10(3), 178-182. https://doi.org/10.38212/2224-6614.2748

Dornenburg, H. (2004). Evaluation of immobilisation effects on metabolic activities and productivity in plant cell processes.
Process Biochemistry, 39(11) 1369-1375. https://doi.org/10.1016/S0032-9592(03)00262-0

Ebrahimi, M., Kiarostami, K., & Bokaee, Z. N. (2019). Effect of salicylic acid on antioxidant properties of in vitro proliferated shoots of Melissa officinalis L. Nova Biologica Reperta, 5(4), 420-427. https://doi.org/10.29252/nbr.5.4.420

Gomez, K.A., & Gomez, A.A. (1984). Statistical procedures for agricutural research. John Wiley and Son. Inc.

Jirakiattikul, Y., Rithichai, P., Songsoem, K., & Itharat, A. (2021). Elicitationof Salicylic Acidon Secondary Metabolite Productionand Antioxidant Activity of In Vitro Musa acuminata L. cv. ‘Gros Michel’ Shoots. Current Applied Science and Technology, 21(3), 569-578.

Kovácik, J., Grúz, J., Baèkor, M., Strnad, M., & Repcák, M. (2009). Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants. Plant Cell Rep., 28, 135-143. https://doi.org/10.1007/s00299-008-0627-5

Markwell, J., Osterman, J., & Mitchell, J. (1995). Calibration of the Minolta SPAD-502
leaf chlorophyll meter. Photosynth. Res., 46,
467–472.

Mendoza, D., Cuaspuda, O., Ariasa, J. P., Ruizc, O., & Arias M. (2018). Effect of salicylic acid and methyl jasmonate in the production of phenolic compounds in plant cell suspension cultures of Thevetia peruviana. Biotechnology reports 19, 1-9). https://doi.org/10.1016/j.btre.2018.e00273

Miura, K., & Tada, Y. (2014). Regulation of water, salinity, and cold stress responses by salicylic acid. Front. Plant Sci., 5, 4. https://doi.org/10.3389/fpls.2014.00004

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Murthy, H. N., Lee, E. J., & Paek, K. Y. (2014). Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture, 118(1), 1-16. https://doi.org/10.1007/s11240-014-0467-7

Nazar, R., Iqbal, N., Syeed, S., & Khan, N.A. (2011). Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mung bean cultivars. J. Plant Physiol., 168, 807-815. https://doi.org/10.1016/j.jplph.2010.11.001

Nurisa, A., Kristanti, A. N., & Manuhara, Y. S. W. (2017). Effect of Sucrose, Erythrose-4-Phosphate and Phenylalanine on Biomassa and Flavonoid Content of Callus Culture from Leaves of Gynura procumbens Merr. The 4th International Conference on Research, Implementation, and Education of Mathematics and Science (4th ICRIEMS). AIP Conf. Proc. 1868, 090013-1–090013-8; doi: 10.1063/1.4995205

Sahaykhare, R., Banerjee, S., & Kundu, K., (2011). Coleus aromaticus Benth – a nutritive medicinal plant of potential therapeutic value. Int. J. Pharm. Biol. Sci., 2(3), 488-500.

Silva, S., Moreira, C.B., Esquibel, M.A., Gil, R., Riehl C., & Sato, A. (2014). Effect of salicylic acid on essential oil compounds of Melissa officinalis in vitro. plants. Tec. Agropecu., 35(1), 178-184.

Zhao, J., Davis, L. C., & Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 23(4), 283-333. https://doi.org/10.1016/j.biotechadv.2005.01.003