Ảnh hưởng của elicitor salicylic acid lên sự sinh trưởng và tích lũy chất biến dưỡng thứ cấp ở cây húng chanh (Plectranthus amboinicus (Lour.) Spreng.) in vitro
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Abstract
Plectranthus amboinicus (Lour.) Spreng., a perennial herb, has antibacterial, anti-inflammatory, and anti-tumor effects which has been used to treat diseases such as respiratory, cardiovascular, and digestive. The study aimed to determine the effective treatment of salicylic acid (SA) to improve the accumulation of in vitro secondary metabolites in this plant. The concentrations of SA at 0, 50, 100, 150 and 200 µM were supplemented into Murashige & Skoog (1962) medium for culturing. The growth and total contents of flavonoids and phenolics in shoot samples were evaluated. The results showed that SA at 50 µM had almost no effect on the shoot growth including leaf number, root formation rate, fresh weight and chlorophyll index. However, it helped to significantly enhance the contents of these two substrates. The total flavonoid content achieved 7.08 mg QE/g dry weight and phenolics reached 2.30 mg GAE/g with the increasing of 1.14 and 1.73-fold compared to the control, respectively. Therefore, exposure to 50 µM SA could help to improve the production of in vitro secondary metabolites in this valuable medicinal plant.
Tóm tắt
Húng chanh hay tần dày lá (Plectranthus amboinicus (Lour.) Spreng. là một loài thảo dược lâu năm có tác dụng kháng khuẩn, chống viêm, kháng u được dùng để chữa các bệnh như hô hấp, tim mạch, tiêu hóa,… Nghiên cứu này nhằm xác định nồng độ salicylic acid (SA) xử lý thích hợp giúp cải thiện sự tích lũy các hợp chất biến dưỡng thứ cấp in vitro ở cây húng chanh. Các nồng độ SA ở 0, 50, 100, 150 và 200 µM được bổ sung vào môi trường nuôi cấy Murashige & Skoog (1962). Sự sinh trưởng và hàm lượng các hợp chất flavonoid và phenolic tổng số trong mẫu chồi được đánh giá. Kết quả cho thấy nồng độ SA 50 µM gần như không tác động lên sự sinh trưởng của chồi bao gồm số lá, tỷ lệ tạo rễ, khối lượng tươi và chỉ số diệp lục tố nhưng có hiệu quả tăng cường hàm lượng của cả hai nhóm hợp chất này. Hàm lượng flavonoid tổng đạt 7,08 mg QE/g khối lượng khô và phenolic đạt 2,30 mg GAE/g, lần lượt tăng gấp 1,14 và 1,73 lần so với đối chứng...
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