Nấm đông trùng hạ thảo Cordyceps militaris: Đặc điểm sinh học, giá trị dược liệu và các yếu tố ảnh hưởng đến quá trình nuôi trồng nấm

NguyễN Thị Liên Thương * , Nguyễn Văn Hiệp và Trịnh Diệp Phương Danh

* Tác giả liên hệ (lienthuong@gmail.com)

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Ngày nhận bài: 20-04-2021
Ngày duyệt đăng: 25-07-2016
Ngày xuất bản: 25-07-2016
Title: Cordyceps militaris: biological characteristics, pharmaceutical values and elements affecting the cultivating process
DOI: 10.22144/ctu.jvn.2016.460
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Thương, N. T. L., Hiệp, N. V., & Danh, T. D. P. (2016). Nấm đông trùng hạ thảo Cordyceps militaris: Đặc điểm sinh học, giá trị dược liệu và các yếu tố ảnh hưởng đến quá trình nuôi trồng nấm. Tạp chí Khoa học Đại học Cần Thơ, (44), 9-22. https://doi.org/10.22144/ctu.jvn.2016.460
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Số. 44 (2016)
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Abstract

Cordyceps militaris is an entomopathogenic fungus with many medicinal values similar to Cordyceps sinensis and has been used for a long time in traditional medicines. Different from Cordyceps sinensis, which has very low mass production and only grows in natural environment, the fungus Cordyceps militaris can be farmed in artificial conditions. Therefore, collecting of technical information and researches will help promoting the production of Cordyceps militaris in Vietnam to satisfy the demand of national and abroad markets. This study evaluated the role of Cordyceps militaris in modern medicine based on summing up the scientific research results, as well as assessing the biological characteristics and the effects of the cultivating conditions for Cordyceps militaris to develop farming processes in our country, which will bring essential economic benefits for local producers. Results from scientific publications showed that there had been many researches confirming the potential applications of C.militaris in illness treatment, also it was widely used in the modern pharmaceutical industry, such as cancer treatment, immunomodulatory, impaired liver and kidney function, etc. (Shonkor, 2010; Seulmee et al., 2009). For the production of C.militaris in artificial conditions, the strict control of environmental conditions such as fungal seeds, temperature, humidity, light, and nutrients was found as essential to maintain the yield and quality C.militaris mushrooms.
Keywords: Cordyceps militaris, culture condition, medicinal value

Tóm tắt

Nấm đông trùng hạ thảo Cordyceps militaris là một loài nấm ký sinh trên côn trùng có giá trị dược liệu quý tương tự như nấm Cordyceps sinensis và được sử dụng nhiều trong y học cổ truyền trong nhiều năm qua. Khác với nấm Cordyceps sinensis với sản lượng rất ít và chỉ mọc trong tự nhiên, loài nấm Cordyceps militaris có thể được nuôi trồng trong điều kiện nhân tạo. Do đo, việc tìm hiểu thông tin kỹ thuật và nghiên cứu về quy trình nuôi trồng sẽ giúp cho việc sản xuất nấm C.militaris thương phẩm tại Việt Nam, đáp ứng nhu cầu thị trường trong và ngoài nước. Nghiên cứu này đánh giá vai trò của nấm đông trùng hạ thảo Cordyceps militaris trong y học hiện đại trên cơ sở tổng hợp nhiều kết quả nghiên cứu khoa học, đồng thời đánh giá các đặc điểm sinh học và ảnh hưởng của các điều kiện nuôi trồng lên loại nấm Cordyceps militaris nhằm phục vụ cho phát triển các quy trình nuôi trồng ở nước ta, đem lại những lợi ích kinh tế thiết thực cho các nhà sản xuất ở các địa phương. Kết quả nghiên cứu từ các công bố khoa học cho thấy nấm C.militaris được nhiều nghiên cứu khẳng định tiềm năng ứng dụng trong điều trị bệnh, đồng thời cũng được sử dụng rộng rãi trong ngành công nghiệp dược phẩm hiện nay, ví dụ như trong điều trị ung thư, điều hòa miễn dịch, suy giảm chức năng gan, thận... (Seulmee et al., 2009; Shonkor, 2010). Đối với việc sản xuất nấm C.militaris ở trong điều kiện nhân tạo, việc kiểm soát chặt chẽ các yếu tố điều kiện môi trường nuôi trồng nấm như giống, nhiệt độ, ẩm độ, ánh sáng và dinh dưỡng là hết sức cần thiết để duy trì sản lượng và chất lượng nấm C.militaris.
Từ khóa: Cordyceps militaris, điều kiện nuôi cấy, giá trị dược liệu

Article Details

Tài liệu tham khảo

Basith M., Madelin M.F., 1968. Studies on the production of perithecial stromata by Cordyceps militaris in artificial culture. Canadian Journal Botany 46:473–480.

Byung-Tae P., Kwang-Heum N., Eui-Cha J., Jae-Wan P., Ha-Hyung K., 2009. Antifungal and Anticancer Activities of a Protein from the Mushroom Cordyceps militaris. Korean Journal of Physiol Pharmacology 13: 49 - 54.

Chang, H. L., Chao, G. R., Chen, C. C., Mau, J. L., 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militarismtcelia. Food Chemistry. 74:203-207.

Che Z.M., 2003. Assessment on edible safety of artificially cultivated Cordyceps militaris fruiting bodies. Edible Fungi. 25(3): 45–46.

Che Z.M., Wang Y., Zhou L.L., 2004. Study on the breeding of a new variety of Cordyceps militaris by mutated with ultraviolet radiation. Food Ferment Industry. 30(8):35–38.

Choi Y.S., Lee H.K., Kim S.H., 1999. Production of fruiting body using cultures of entomopathogenic fungal species. Korean Journal of Mycology. 27: 15–19

Christian G.D.N. E. and Henry A. C. F., 1837. Das System der Pilze: part one.

Das S.K., Masuda M., Mikio S., 2010. Medicinal uses of the mushroom Cordyceps militaris: current state and prospects. Fitoterapia. 81:961–968.

de Bary A., 1887. Comparative morphology and biology of the fungi, mycetozoa and bacteria. Clarendon, Oxford.

Dong JZ, Lei C, Ai XR et al., 2012. Selenium enrichment on Cordy-ceps militaris Link and analysis on its main active components. Applied Biochemistry and Biotechnology. 166:1215–1224.

Du A.L., Zhang X. and Zhang H.Z., 2010. A new high cordycepin Cordyceps militaris cultivar‘Haizhou 1’. Acta Horticulture Sinica. 37:1373–1374.

Farlow W.G., Seymour A.B., 1888. A provisional host-index of the fungi of the United States: part 1. Polypetalae, Cambridge.

Fengyao W., Hui Y., Xiaoning M., Junqing J., Guozheng Zh., Xijie G. and Zhongzheng G., 2011. Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris. African Journal of Microbiology Research. 5(18): 2743-2751.

Gao S.Y., Wang F.Z., 2008. Research of commercialized cultivation technology on Cordyceps militaris. North Hortic 9:212–215.

Gao X.H., Wu W., Qian G.C., 2000. Study on influences of abiotic factors on fruitbody differentiation of Cordyceps militaris. Acta Agriculture Shanghai. 16: 93–98.

Geng L.J., He L.L., Yu-Yang E., 2009. Effect of different preservation conditions on mycelial growth and fruitbody yield of Cordyceps militaris. Journal of Shenyang Agriculture University. 40:165–168.

Gong C.L., Pan Z.H., Zheng X.J., 2006a. Anti-oxidation of cultured Cordyceps militaris growing on silkworm pupa. In: Proceedings of International Workshop on Silk handcrafts cottage industries and silk enterprises development in Africa, Europe, Central Asia and the Near East, & Second Executive Meeting of Black, Cas-pian seas and Central Asia Silk Association (BACSA), Bursa, Turkey, pp 615−620.

Gong C.L., Pan Z.H., Zheng X.J., 2006b. Immunoregulation function of artificially Cordyceps militaris growing on pupae of silkworm Bombyx mori for mice. In: Proceedings of International Workshop on Silk handcrafts cottage industries and silk enterprises develop-ment in Africa, Europe, Central Asia and the Near East, & Second Executive Meeting of Black, Caspian seas and Central Asia Silk Association (BACSA), Bursa, Turkey, pp 633−638.

Gu Y.X., Wang Z.S., Li S.X., 2007. Effect of multiple factors on accumulation of nucleosides and bases in Cordyceps militaris. Food Chemistry 102:1304–1309.

Halpern G.M., 1999. Cordyceps: China’s healing mushroom. Avery, New York

Harada Y., Akiyama N., Yamamoto K., Shirota Y., 1995. Production of Cordyceps militaris fruit body on artificially inoculated pupae of Mamestra brassicae in the laboratory. Transactions of the Mycological Society of Japan. 36: 67–72.

Hong I.P., Kang P.D., Kim K.Y., 2010. Fruit body formation on silkworm by Cordyceps militaris. Mycobiology. 38:128–132.

Huang N.L., Lin Z.B., Chen G.L., 2010. Medicinal and edible fungi. Shanghai Scientific and Technological Literature, Shanghai.

Huang S.J., Tsai S.Y., Lee Y.L., 2006. Nonvolatile taste components of fruiting bodies and mycelia of Cordyceps militaris. Food Science Technology 39:577–583.

Hur H., 2008. Chemical Ingredients of Cordyceps militaris. Mycobiology. 36(4):233-235.

Jae-Sung K., Kumar S., Se-Eun P., Bong-Suk C.i, Seung K., Nguyen T. H., Chun-Sung K., Han-Seok C., Myung-Kon K., Hong-Sung C., Yeal P., Sung-Jun K., 2006. A Fibrinolytic Enzyme from the Medicinal Mushroom Cordyceps militaris. Journal of Microbiology 44(6):622-31.

Jones K., 1997. Cordyceps: tonic food of ancient China. Sylvan, Washington.

Kamble V.R. and Agre D.G., 2012. Reinvestigation of insect parasite fungus Cordyceps militaris from Maharashtra. Bionano Frontier 5(2):224-225.

Kim S.W., Hwang H.J., Xu C.P., 2003. Optimization of submerged culture process for the production of mycelial biomass and exo-polysaccharides by Cordyceps militaris C738. Journal of Apply Microbiology. 94:120–126.

Kobayasi Y., 1941. The genus Cordyceps and its allies. Science Reports of the Tokyo Bunrika Daigaku section B. 84(5):53–260.

Kobayasi Y., 1981. Revision of the genus Cordyceps and its allies 1. Bulletin of the National Science Museum Tokyo. 7:1–13.

Kobayasi Y., 1982. Keys to the taxa of the genera Cordyceps and Torrubiella. Transactions of the Mycological Society of Japan 23:329–364 Herbal, New York.

Kwon J.S., Lee J.S., Shin W.C., 2009. Optimization of culture conditions and medium components for the production of mycelial biomass and exopolysaccharides with Cordyceps militaris in liquid culture. Biotechnology and Bioprocess Engineering. 14:756–762.

Khan M.A., Tania M., Zhang D.Z., 2010. Cordyceps mushroom: a potent anticancer nutraceutical. Open Nutraceuticals Journal. 3:179–183

Leatherdale D., 1970. The arthropod hosts of entomogenous fungi in Britain. Entomophaga. 15:419–435.

Li C.B., Tong X.D., Bai J., 2004. Artificial stromata production of Cordyceps militaris. Journal of Dalian National University. 6(5):29–31.

Li C.L., Liu X.L., Zheng X.Q., 2011. Mutagenesis of Cordyceps militaris protoplast for high production of exopolysaccharide. Indian Microbiology. 41(2):51–56.

Li C.R., Nam .S.H., Geng D.G., 2006. Artificial culture of seventeen Cordyceps spp. Mycosystema. 25:639–645.

Li J., Chen G.S., Fang Q.M., 2010. Comparative study on cultivated Cordyceps militaris and wild Cordyceps sinensis. Journal of Chengdu University. 33(3):82–84.

Li M.N., Wu X.J., Li C.Y., 2003. Molecular analysis of degeneration of artificial planted Cordyceps militaris. Mycosystema 22:277–282.

Li N., Song J.G., Liu J.Y., Zhang H., 1995. Compared chemical composition between Cordyceps militaris and Cordycpes sinensis. Journal of Jilin Agriculture University 17, 80–3. (in Chinese).

Li S.P., Yang F.Q., Tsim K.W.K., 2006. Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. Journal of Pharmaceutical and Biomedical Analysis, 41, 1571–84.

Lim L., Lee C., Chang E., 2012. Optimization of solid state culture conditions for the production of adenosine, cordycepin, and D-mannitol in fruiting bodies of medicinal caterpillar fungus Cordyceps militaris (L.:Fr.) Link (Ascomycetes). International Journal of Medicinal Mushrooms. 14:181–188.

Lin Q.Q., Qiu X.H., Zheng Z.L., 2010. Characteristics of the degenerate strains of Cordyceps militaris. Mycosystema 29:670–677.

Lin W.H., Tsai M.T., Chen Y.S., Hou R.C., Hung H.F., Li C.H., Wang H.K., Lai M.N., Jeng K.C., 2007. Improvement in sperm production in subfertile boars by Cordyceps militaris. The American Journal of Chinese Medicine.35(4):631-41.

Liu D., He L.L., Wang Z.Q., 2006. The influences of subculture of Cordyceps militaris to colonial morphology and fruitbody yield. Journal of Shenyang Agriculture University. 37:538–541.

Liu M.M., Ning S.Y., Cui X.Y., 2008. Optimization of submerged culture condition for Cordyceps militaris using response surface methodology. China Agricultural Science. 24(5):127–131.

Liu X.L., Zhou J.Z., Huang K.H., 2009. Study on preparation and regeneration of protoplast of Cordyceps militaris. Acta Agriculturae Universitatis Jiangxiens. 21(9):119–120.

Liu Z.Y., Yao Y.J., Liang Z.Q., 2001. Molecular evidence for the anamorphteleomorph connection in Cordyceps sinensis. Mycological Research. 105: 827–32.

Lu J.M., Zeng Z.J., He H.Q., 2005. Culture technique of Cordyceps militaris on artificial media. Guangdong Agricultural Science. 2:88–89.

Ma L.P., Zhao J.F., Liu K.Y., 2008. Research on the isolation condition of Cordyceps militaris protoplast. Journal of Anhui Agricultural Science 36:14612–14613.

Ma T., Feng Y., Wu X.P., 2007. Primary investigation of a host insect of Cordyceps militaris and analysis of its main ingredients. Forest Resources 20:63–67.

Mains E.B., 1958. North American entomogenous species of Cordyceps. Mycologia. 50:169–222.

McKenna D.J., Jones K., Hughes K., 2002. Botanical medicines: the desk reference for major herbal supplements, 2nd edn. Haworth.

Mizuno T., 1999. Medicinal Effects and Utilization of Cordyceps (Fr.) Link (Ascomycetes) and Isaria Fr. (Mitosporic Fungi) Chineese Caterpiller Fungi, “Tochukaso” (Review). International Journal of Medicinal Mushrooms. 1:251-261.

Moore R.T., 1964. Fine structure of mycota: 12 Karyochorisis – somatic nuclear division – in Cordyceps militaris. Z Zellforsch. 63:921–937.

Müller-Kögler E., 1965. Cordyceps militaris (Fr.) Link: Beobachtun-gen und Versuche anlässlich eines Fundes aufTipula paludosa Meig. (Dipt., Tipul.). Z Angew Entomol. 55:409–418.

Ni H., Li H.H., Huang W.F., 2007. Research and product develop-ment of Cordyceps militaris and its bioactive substances. Review Science and Technology 25(15):75–79.

Panigrahi A., 1995. Fungus C. militaris infestation in the pupa of the tea pest Andraca bipunctata Walker. Environmental Ecology. 13:942–946.

Paterson R.R.M., 2008. Cordyceps—a traditional Chinese medicine and another fungal therapeutic biofactory? Phytochemistry. 69:1469–1495.

Paul M. K., Paul F. C., David W. M. and Stalpers J. A., 2008. Dictionary of the Fungi; CABI.

Peng Zh.,Yongliang X., Guohua X., Chenghui X., Xiao H., Siwei Zh., Huajun Zh., Yin Huang, Yan Zh., Shengyue W., Guo-Ping Zh., Xingzhong L., Raymond J. St L. and Chengshu W., 2011. Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional chinese medicine. Genome Biology,12: 116.

Ren W.Y., Zhao H., Wu Z.K., 2009. Techniques for fast and high yielding cultivation of the valuable edible and medicinal mushroom, Cordyceps militaris. China Agricultural Technology Extension. 25(5):28–29.

Sato H. and Shimazu M., 2002. Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Applied Entomology and Zoology. 37:85–92.

Seulmee S., Sungwon L., Jeonghak K., Sunhee M., Seungjeong L., Chong-Kil L., Kyunghae C., Nam-Joo H., Kyungjae K., 2009. Cordycepin Suppresses Expression of Diabetes Regulating Genes by Inhibition of Lipopolysaccharide-induced Inflammation in Macrophages. Immune Network. 9(3):98-105.

Shanor L., 1936. The production of mature perithecia of Cordyceps militaris (Linn.) Link in laboratory culture. Journal of the Elisha Mitchell Scientific Society. 52:99–105.

Shih I.L., Tsai K.L., Hsieh C.Y., 2007. Effects of culture conditions on the mycelial growth and bioactive metabolite production in submerged culture of Cordyceps militaris. Biochemical Engineering Journal. 33, 193–201.

Shonkor K. D., Shinya F., Mina M. and Akihiko S., 2010. Efficient Production of Anticancer Agent Cordycepin by Repeated Batch Culture of Cordyceps militaris Mutant. Lecture Notes in Engineering and Computer Science. 20-22.

Shrestha B., Park Y.J., Han S.K., 2004. Instability in vitro fruiting of Cordyceps militaris. Journal of Mushroom Science and Production. 2:140–144.

Shrestha B., Sung J.M., 2005. Notes on Cordyceps species collected from central region of Nepal. Mycobiology 33:235–239.

Shrestha B., Zhang W.M., Zhang Y.J., 2010. What is the Chinese caterpillar fungus Ophiocordyceps sinensis (Ophiocordycipitaceae)?.Mycology. 1:228–236.

Shrestha B., Lee W.H., Han S.K., Sung J.M., 2006. Observations on Some of the Mycelial Growth and Pigmentation Characteristics of Cordyceps militaris Isolates. Mycobiology. 34(2):83-91.

Stensrud Ø., Hywel-Jones N.L., Schumacher T., 2005. Towards a phylogenetic classification of Cordyceps: ITS nrDNA sequence data confirm divergent lineages and paraphyly. Mycological Research. 109: 41–56.

Stone R., 2008. Last stand for the body snatcher of the Himalayas? Science. 322:1182.

Sung G.H., Spatafora J.W., 2004. Cordyceps cardinali ssp. nov., a new species of Cordyceps with an east Asian-eastern North American distribution. Mycologia 96:658–666.

Sung J.M., 1996. The insects-born fungus of Korea in color. Kyohak Publishing Co. Ltd., Seoul.

Sung J.M., Choi Y.S., Lee H.K., 1999. Production of fruiting body using cultures of entomopathogenic fungal species. Korean Journal of Mycology 27:15–19.

Sung J.M., Choi Y.S., Shrestha B., Park Y.J., 2002. Investigation on artificial fruiting of Cordyceps militaris. Korean Journal of Mycology. 30: 6–10.

Sung J.M., Park Y.J., Han S.K., 2006. Selection of superior strains of Cordyceps militaris with enhanced fruiting body productivity. Mycobiology. 34: 131–137.

Sung J.M., Park Y.J., Lee J.O., 2007. Effect of preservation periods and subcultures on fruiting body formation of Cordyceps militaris in vitro. Mycobiology 34:196–199 Supplements. American Journal of Chinese Medicine. 35(4):631-41.

Wang G.D., 1995. Ecology, cultivation and application of Cordyceps and Cordyceps sinensis. Scientific and Technical Documents, Beijing.

Wang H.J., Chu Z., Feng L., 2010. A comparative study on effect of two plant growth promoters on the growth of Cordyceps militaris. Lishizhen Medicine And Material Medical Research. 21:541–542.

Wang J.F., Yang C.Q., 2006. Research survey on artificial cultivation and product development of Cordyceps militaris. Lishizhen Medicine And Material Medical Research. 17:268–269.

Wang L., Zhang W.M., Hu B., 2008. Genetic variation of Cordyceps militaris and its allies based on phylogenetic analysis of rDNA ITS sequence data. Fungal Divers 31:147–155.

Wang X.L., Yao Y.J., 2011. Host insect species of Ophiocordyceps sinensis: a review. ZooKeys. 127:43–59.

Wang X.Q., Chen C.Q., Zhang R., 2002. Methodological studies on cultivation of Cordyceps militaris on pupae of Antheraea pernyi. Journal of Anhui Agricultural Science. 30:965-968.

Wol-Soon J., Yoo-Jin C., Hyoun-Ji K., Jae-Yun L., Byung-Hyouk N., Jae-Dong L., Sang-Wha L., Su-Yeong S. and Min-Ho J., 2010 .The Anti-inflammatory Effects of Water Extract from Cordyceps militaris in Murine Macrophage. Mycobiology. 38(1): 46-51.

Xie C.Y., Gu Z.X., Fan G.J., 2009a. Production of cordycepin and mycelia by submerged fermentation of Cordyceps militaris in mixture natural culture. Applied Biochemistry and Biotechnology. 158:483–492.

Xie C.Y., Liu G.X., Gu Z.X., 2009b. Effects of culture conditions on mycelium biomass and intracellular cordycepin production of Cordyceps militaris in natural medium. Annua Microbiology. 59:293–299.

Xiong C.H., Xia Y.L., Zheng P., 2010. Developmental stage-specific gene expression profiling for a medicinal fungus Cordyceps militaris. Mycology 1:25–66.

Yahagi N., Yahagi R., Takano F., 2004. Growth of ascoscarps from cultured Cordyceps militaris(L.:Fr.) Fr. and Cordyceps formicarum Kobayasi in an agar medium. Nippon Kingakukai Kaiho. 45:15–19.

Yan H., Zhu D., Xu D., Wu J. and Bian X., 2008. A study on Cordyceps militaris polysaccharide purification, composition and activity analysis. African Journal of Biotechnology. 7 (22): 4004-4009.

Young-Joon A., Suck-Joon P., Sang-Gil L., Sang-Cheol S. and Don-Ha C., 2000. Cordycepin: Selective Growth Inhibitor Derived from Liquid Culture of Cordyceps militaris against Clostridium spp. Journal of Agricultural and Food Chemistry. 48: 2744−2748.

Yu H.M., Wang B.S., Huang S.C., 2006. Comparison of protective effects between cultured Cordyceps militaris and natural Cordyceps sinensis against oxidative damage. Journal of Agriculture and Food Chemistry. 54:3132–3138.

Yue D.C., Yang Y.P., Wang S.F., 1982. Preliminary study on stroma formation of Cordyceps militaris strain (Briefing). Journal of China Medicinal Materrial. 05:7

Yue G.G.L., Lau C.B.S., Fung K.P., 2008. Effects of Cordyceps sinensis, Cordyceps militaris and their isolated compounds on ion transport in Calu-3 human airway epithelial cells. Journal of Ethno-pharmacology. 117:92–101.

Yuko O., Jung-Bum L., Kyoko H., Akio F., Dong-Ki P. and Toshimitsu H., 2007. In Vivo Anti-influenza Virus Activity of an Immunomodulatory Acidic Polysaccharide Isolated from Cordyceps militaris Grown on Germinated Soybeans. Journal of Agricultural and Food Chemistry. 55: 10194–10199.

Zhang J.Y., Wu K.L., Duan J., 2010. Influence of air permeability on growth of Cordyceps militaris. Guangdong Agricultural Science. 4:45–47.

Zhang X.K. and Liu W.X., 1997. Experimental studies on planting Cordyceps militaris (L. ex Fr.) Link with different culture materials. Edible Fungi China. 16(2):21–22.

Zhang Y.J., Li E., Wang C.S., 2012. Ophiocordyceps sinensis, the flagship fungus of China: terminology, life strategy and ecology. Mycology. 3:2–10.

Zhang Z., Lei Z., Lu Y., 2008. Chemical composition and bioactivity changes in stale rice after fermentation with Cordyceps sinensis. Journal of Bioscience and Bioengineering. 106: 188–93.

Zheng P., Xia Y.L., Xiao Ch.H., 2011. Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional Chinese medicine. Genome Biology. 23; 12.

Zhong S., Pan H.J., Fan L.F., 2009. Advances in research of polysaccharides in Cordyceps species. Food Technology & Biotechnology. 47:304–312.

Zhou H.Y., Bian Y.B., 2007. Identification and selection of high cordycepin-yielding protoplast fusion products of Cordyceps militaris. Acta Edulis Fungi. 14(2):65–70.

Zhou L.H., Luo L.M., 2009. Preparation and regeneration of protoplasts from Cordyceps militaris. Hubei Agricultual Science. 48:1621–1624.