Sự bài tiết ammonia và nitrite của tôm thẻ chân trắng (Litopenaeus vannamei) trong môi trường có độ mặn khác nhau
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Abstract
The study aimed to determine ammonia and nitrite excretion levels of whiteleg shrimp (Litopenaeus vannamei) in environments with different salinities. The experiments were arranged with 5 treatments (5, 10, 15, 20 and 25‰), 3 replications for each. Water with different salinities was mixed from concentrated seawater and tap water. The experiments were carried out in glass tanks containing 25 liters of water with a density of 10 shrimp/tank (1,5 ± 0,13g/shrimp) and 16 shrimp/tank (4,41 ± 0,77g/shrimp). Shrimp were fed ad libitum one hour before the experiment was conducted and not fed during the experiment. Water and blood samples were collected at 0, 1, 3, 6, 12, 24, 48, and 96 hours to measure TAN (phenate method 4500-NH3 F.) and NO2--N (colorimetric method 4500-NO2- B.) concentration. The results showed that NO2- was one of the excretory products of white-leg shrimp and shrimp excrete NO2- directly into the water environment. On average, shrimp excrete 25.32 mg TAN/g/h and 1.22 mg NO2--N/g/h. The TAN and NO2--N concentrations in the shrimp blood were 0.85 mg/mL and 0.052 mg/mL. Salinity has affected TAN, N-NO2- excrete level and TAN concentration in the shrimp blood, but the effect did not follow a clear pattern. Salinity has not impacted on NO2--N concentration in the shrimp blood.
Tóm tắt
Mục tiêu được đề ra trong nghiên cứu nhằm xác định được mức độ bài tiết ammonia và nitrite của tôm thẻ chân trắng (Litopenaeus vannamei)trong các môi trường có độ mặn khác nhau.Các thí nghiệm được bố trí gồm 5 nghiệm thức (5, 10, 15, 20 và 25%)với 3 lần lặp lại. Nước với các độ mặn khác nhau được pha từ nước ót và nước máy. Thí nghiệm được thực hiện trên bể kính chứa 25 lít nước với mật độ 10 con/bể (1,5 ± 0,13g/con)và 16 con/bể (4,41 ± 0,77g/con).Tôm được cho ăn no một giờ trước khi bố trí và không cho ăn trong thời gian thí nghiệm.Mẫu nước và mẫu máu tôm được thu ở các thời điểm 0, 1, 3, 6, 12, 24, 48 và 96 giờ để đo hàm lượng TAN (phương pháp phenate 4500-NH3 F.) và N-NO2-(phương pháp so màu 4500-NO2- B.)Kết quả cho thấy NO2- là một trong những sản phẩm bài tiết của tôm thẻ chân trắng, tôm bài tiết NO2- trực tiếp ra môi trường nước. Trung bình, tôm bài tiết 25,32 mg TAN/g/h và 1,22 mg N-NO2-/g/h. Hàm lượng TAN và và N-NO2- trong bình trong máu tôm là 0,85 mg/mL và 0,052 mg/mL. Độ mặn có ảnh hưởng đến mức độ bài tiết TAN, N-NO2- và hàm lượng TAN trong máu tôm nhưng sự tác động của độ mặn không theo một quy luật rõ ràng.Độ mặn không ảnh hưởng đến hàm lượng N-NO2- trong máu tôm.
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