Phân lập và tuyển chọn vi khuẩn chịu mặn từ nốt sần một số cây họ đậu ở khu vực đồng bằng sông Cửu Long
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Abstract
The study aimed to isolate and select salt tolerant bacteria from the nodules of leguminous plants such as Macroptilum lathyroides, Clitoria marianna, Sesbania sesban, Arachis pintoi and Aeschynomene indica. YEMA medium supplemented with 1% NaCl was used to isolate bacteria. As a result, 10 strains were isolated through identification characteristics, including Gram-negative, rod-shaped, motile, aerobic, non-spore-forming, and non-staining on YEMA medium supplemented with 0.025% Congo Red. In addition, they are resistant to salt in liquid culture medium with NaCl concentrations ranging from 2.5% to 10%. Among them, three strains LĐ6, TL3, and BV8 showed good salt tolerance up to 10% NaCl. Based on the results of 16S rDNA gene sequencing, strain TL3 was identified as Rhizobium sp. TL3, while LD6 and BV8 were characterised as Enterobacter sp. LD6 and Enterobacter sp. BV8, respectively.
Tóm tắt
Nghiên cứu được thực hiện nhằm phân lập và tuyển chọn chủng vi khuẩn chịu mặn từ nốt sần của Đậu hoang bông đỏ (Macroptilum lathyroides), Đậu hoang bông tím (Clitoria marianna), Điền thanh (Sesbania sesban), Cỏ lá đậu (Arachis pintoi) và Điền ma Ấn (Aeschynomene indica). Môi trường YEMA bổ sung 1% NaCl được dùng để phân lập vi khuẩn. Kết quả cho thấy, 10 dòng vi khuẩn đã được phân lập thông qua đặc tính nhận dạng gồm: Gram âm, hình que, di động, hiếu khí, không hình thành bào tử và không bắt màu trên môi trường YEMA có bổ sung 0,025% Congo Red. Ngoài ra, chúng có khả năng chịu mặn trong môi trường nuôi cấy lỏng có nồng độ NaCl dao động từ 2,5% đến 10%. Trong đó ba dòng LĐ6, TL3 và BV8 thể hiện khả năng chịu mặn tốt lên đến 10% NaCl. Dựa vào kết quả giải trình tự đoạn gen 16S rRNA, dòng TL3 được định danh với tên Rhizobium sp. TL3, trong khi LĐ6 và BV8 lần lượt là Enterobacter sp. LĐ6 và Enterobacter sp. BV8.
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