Khảo sát hoạt tính chống oxy hóa và kháng viêm in vitro của dịch chiết ngoại bào vi khuẩn nội sinh phân lập từ cây mảnh cộng (Clinacanthus nutans)
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Abstract
This study aimed to isolate endophytic bacteria (EB) from Clinacanthus nutans with the ability to synthesize compounds possessing antioxidant and anti-inflammatory properties. The antioxidant activity of extracellular extracts (EE) from EB was evaluated based on total phenolic content, total polyphenol content, DPPH free radical scavenging, total antioxidant capacity, and reducing power. Anti-inflammatory activity was assessed through inhibition of bovine serum albumin denaturation. The study isolated 24 EB strains that could synthesize extracellular polyphenol compounds with antioxidant and anti-inflammatory properties. Total polyphenol content (TPC) ranged from 94.32 to 2284.44 mg GAE/mL EE, and total flavonoid content (TFC) ranged from 5.35 to 99.69 mg QE/mL EE. Antioxidant activity measured by DPPH, reducing power (RP), and total antioxidant capacity (TAC) ranged from 0.13 to 1067.36 mg vitamin C equivalent/mL EE. Anti-inflammatory content ranged from 0.32 to 0.46 mg diclofenac equivalent/mL EE. The three EB strains with consistently high activity across all parameters were identified as Bacillus sp. CN-R1, Enterobacter sp. CN-R15, and Enterobacter sp. CN-L7.
Tóm tắt
Mục tiêu nghiên cứu là phân lập vi khuẩn nội sinh (VKNS) có khả năng sinh tổng hợp các hợp chất chống oxy hóa và kháng viêm ở cây Mảnh Cộng (Clinacanthus nutans). Hoạt tính chống oxy hóa của dịch chiết ngoại bào (DCNB) từ VKNS được đánh giá qua hàm lượng polyphenol, flavonoid tổng số, khả năng trung hòa gốc tự do DPPH, khả năng chống oxy hóa tổng số và năng lực khử. Khả năng kháng viêm được xác định qua ức chế sự biến tính của albumin huyết thanh bò. Kết quả đã phân lập được 24 dòng VKNS có khả năng sinh tổng hợp các hợp chất polyphenol ngoại bào, chống oxy hóa và kháng viêm. Hàm lượng TPC từ 94,32 đến 2284,44 mg GAE/mL DCNB, TFC từ 5,35 đến 99,69 mg QE/mL DCNB. Khả năng chống oxy hóa bởi phương pháp DPPH, RP và TAC từ 0,13 đến 1067,36 mg vitamin C tương đương/mL DCNB. Hàm lượng chất kháng viêm từ 0,32 đến 0,46 mg diclofenac tương đương/mL DCNB. Ba dòng VKNS có hoạt tính cao đồng đều ở các chỉ tiêu khảo sát là Bacillus sp. CN-R1, Enterobacter sp. CN-R15 và Enterobacter sp. CN-L7.
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