Khảo sát tương tác của tuyến trùng Meloidogyne incognita và nấm Fusarium oxysporum f.sp. cubense trên cây chuối già Nam Mỹ (Musa acuminata) trong điều kiện nhà lưới
,
và
Article Sidebar
Full Text: 
PDF
Abstract
Investigation of the interaction between the root-knot nematode Meloidogyne incognita and the fungus Fusarium oxysporum f.sp. cubense cause Panama wilt disease on Cavendish bananas Musa cavendish “Grande Naine”. This study aimed to evaluate the simultaneous interaction of two pathogens and how the sequential increase in nematode populations affects the symptoms of Panama wilt. The results showed that simultaneously infected with two nematode and fungal agents had lower plant growth parameters and showed more severe symptoms of wilting and nutritional deficiency compared with the experimental ones infected individually. This level increased rapidly and was aggravated by the increased number of nematodes present simultaneously. In the treatment with the nematode M. incognita (1 individual/g soil) and the fungus Fusarium oxysporum (106 spores/mL), the plant growth was reduced and lower than that of the treatment alone, and the symptoms increased and wilting was proportional to the number of nematodes M. incognita. However, in the experiment infecting 4 nematodes/g of soil interacting with 106 fungal spores/mL, the density of nematodes increased the least compared to the other infection experiments, showing that with the density of infection. Initially high, there was a competition between the two parasitic agents.
Tóm tắt
Nghiên cứu được thực hiện nhằm khảo sát sự tương tác giữa tuyến trùng bướu rễ Meloidogyne incognita và nấm Fusarium oxysporum f.sp. cubense gây bệnh héo rũ Panama trên giống chuối già Nam Mỹ Musa cavendish “Grande Naine” đánh giá sự tương tác đồng thời của hai tác nhân mầm bệnh và sự gia tăng tuần tự của mật số tuyến trùng đến triệu chứng héo rũ Panama. Kết quả ghi nhận tại các thí nghiệm được lây nhiễm kết hợp đồng thời hai tác nhân tuyến trùng và nấm bệnh đều có chỉ tiêu sinh trưởng của cây thấp hơn, biểu hiện triệu chứng héo rũ và/hoặc thiếu dinh dưỡng nặng hơn so với khi lây nhiễm riêng lẻ, mức độ này tăng nhanh và trầm trọng hơn theo sự gia tăng mật số tuyến trùng hiện diện đồng thời. Nghiệm thức mật độ tuyến trùng Meloidogyne incognita 1 con/g đất và nấm Fusarium oxysporum 106 bào tử/mL thì sự phát triển của cây đã giảm và thấp hơn so với nghiệm thức chỉ xâm nhiễm riêng lẻ và sự gia tăng triệu chứng héo rũ tỷ lệ thuận với mật số tuyến trùng M. incognita. Tuy nhiên, thí nghiệm lây nhiễm 4 con tuyến trùng/g đất tương tác với 106 bào tử nấm/mL thì mật độ tuyến trùng gia tăng thấp nhất cho thấy với mật độ lây nhiễm ban đầu cao đã có sự cạnh tranh giữa hai tác nhân ký sinh.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Tài liệu tham khảo
Agrios, G. N. (2005). Plant pathology. Elsevier.
Andree Vega-Callo, R., Yaquelin Mendoza-Lima, M., Ruth Mamani-Mendoza, N., Sharon Lozada-Villanueva, L., Jose Tamo-Zegarra, J., Gloria Casa-Ruiz, T., & Belle, C. (2020). First report of southern root-knot nematode, Meloidogyne incognita, infecting pomegranate, Punica granatum, in Peru. Journal of Nematology, 52. https://doi.org/10.21307/jofnem-2020-026
Arias, P. (2003). The World Banana Economy, 1985-2002 (No. 1). Food & Agriculture Org.
Barker, K. R. (1985). Nematode extraction and bioassays. In: An advanced treatise on Meloidogyne, Volume II – Methodology. In Barker, K. R., Carter, C. C., & Sasser, J. N. (Eds). A Cooperative Publication of the Department of Plant Pathology, North Carolina State University and the U. S. Agency for International Development, USA (pp. 19-32).
Beckmann, J., Körber, C., Rau, G., Hubel, A., & Cravalho, E. G. (1990). Redefining cooling rate in terms of ice front velocity and thermal gradient: first evidence of relevance to freezing injury of lymphocytes. Cryobiology, 27(3), 279-287.
https://doi.org/10.1016/0011-2240(90)90027-2
Burgress, L. W., Knight, T. E., Tesoriero, L., & & Phan, H. T. (2009). Cẩm nang chẩn đoán bệnh cây ở Việt Nam. ACIAR: Canberra.
Byrd, J. R. D. W., Kirkpatrick, T., & Barker, K. (1983). An improved technique for clearing and staining plant tissues for detection of nematodes. Journal of nematology, 15(1), 142.
Cabasan, M. T. N., Kumar, A., Bellafiore, S., & De Waele, D. (2014). Histopathology of the rice root-knot nematode, Meloidogyne graminicola, on Oryza sativa and O. glaberrima. Nematology, 16(1), 73-81.
https://doi.org/10.1163/15685411-00002746
Castella, G., Bragulat, M. R., Rubiales, M. V., & Caba es, F. J. (1997). Development of a selective culture medium for Fusarium moniliforme. MICROBIOLOGIA-MADRID-, 13, 493-498.
Eco-Fruits (2020). Vietnam Green Cavendish Banana: Step-by-step dominate the market. https://eco-fruits.com/vietnam-green-cavendish-banana-step-by-step-dominate-the-market/ (March 4, 2020).
Eisenback, J. D., Hirschmann, H., Sasser, J. N., & Triantaphyllou, A. C. (1981). A guide to the four most common species of root-knot nematodes (Meloidogyne species) with a pictorial key. U.S. Agency for International Development (pp.48).
Gao, X., Jackson, T. A., Hartman, G. L., & Niblack, T. L. (2006). Interactions between the soybean cyst nematode and Fusarium solani f.sp. glycines based on greenhouse factorial experiments. Phytopathology, 96(12), 1409-1415. https://doi.org/10.1094/PHYTO-96-1409
Gowen, S., & Quénéhervé, P. (1990). Nematode Parasites of Bananas, Plantains and Abaca, 431-460. In Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (Eds. M. Luc, R. A. Sikora & Bridge, J.). CAB International, Wallingford, UK, (pp. 896) pp.
Griffin, G. D., & Thyr, B. D. (1988). Interaction of Meloidogyne hapla and Fusarium oxysporum f. sp. medicaginis on alfalfa. Phytopathology, 78(4), 421-425.
https://doi.org/10.1094/Phyto-78-421
Hartman, K. M., & Sasser, J. N. (1985). Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. In: An advanced treatise on Meloidogyne, Volume II. Methodology (Barker, K. R., Carter, C. C., & Sasser, J. N. (editors)). A Cooperative Publication of the Department of Plant Pathology, North Carolina State University and the U.S. Agency for International Development (pp. 69-78).
Hofmann, J., & Grundler, F. M. W. (2007). How do nematodes get their sweets? Solute supply to sedentary plant-parasitic nematodes. Nematology, 9, 451-458. https://doi.org/10.1163/156854107781487305
Hooper, D. J., Hallmann, J. and & Subbotin, S. A. (2005). Methods for extraction, processing and detection of plant and soil nematodes. In: Plant parasitic nematodes in subtropical and tropical agriculture (2nd edition) (M. Luc, M., Sikora, R. A. and Bridge, J. (editors)). CAB International. (pp. 53 -– 80). https://doi.org/10.1079/9780851997278.0053
Hunt, D. J., & Handoo, Z. A. (2009). Taxonomy, identification and principal species. In Root-knot nematodes (pp. 55-97). Wallingford UK: CABI.
Hussey, R. S., & Janssen, G. J. W. (2002). Root-knot nematode: Meloidogyne species. In: Starr JL, Cook R, Bridge J, editors. Plant Resistance to Parasitic Nematodes. Wallingford, UK: CAB International (pp. 43-70). DOI. 10.1079/9780851994666.0043
INIBAP (2000). International Network for the Improvement of Banana and Plantain (2000) Networking banana and plantain. 64 p. 521.
Jones, D. R. (2000). Diseases of banana, Abacá and Enset. CABI Publishing, Wallingford.
Jones, J. T., Haegeman, A., Danchin, E. G., Gaur, H. S., Helder, J., Jones, M. G., Kikuchi, T., Manzanilla‐López, R., Palomares‐Rius, J. E., Wesemael, W. M., & Perry, R. N. (2013). Top 10 plant‐parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14, 946-961. https://doi.org/10.1111/mpp.12057
Khan, M. R., Pal, S., Manohar, G. T., Bhattacharyya, S., Singh, A., Sarkar, P., & Lalliansanga, S. (2017). Detection, diagnosis and pathogenic potential of Meloidogyne incognita on passion fruit from mizoram, India. Pakistan Journal of Zoology, 49(4). https://doi.org/10.17582/journal.pjz/2017.49.4.1207.1214
Knogge, W. (1996). Fungal infection of plants. The Plant Cell, 8(10), 1711. https://doi.org/10.2307/3870224
Mak, C., Mohamed, A. A., Liew, K. W., & Ho, Y. W. (2004). Early screening technique for Fusarium wilt resistance in banana micropropagated plants. In Banana improvement: cellular, molecular biology, and induced mutations. Proceedings of a meeting held in Leuven, Belgium, 24-28 September 2001, (pp. 219-227). Science Publishers, Inc.
Classic Murashige, C. T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15, 473-497.
https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Châu, N. N., & Thanh, N. V. (2000). Động vật chí Việt Nam: Tuyến trùng ký sinh thực vật, tập 4. Việt Nam: Nhà xuất bản Khoa học và Kỹ thuật.
Oka, Y., Ben‐Daniel, B., & Cohen, Y. (2012). Nematicidal activity of the leaf powder and extracts of Myrtus communis against the root‐knot nematode Meloidogyne javanica. Plant pathology, 61(6), 1012-1020.
https://doi.org/10.1111/j.1365-3059.2011.02587.x
Pattison, A. B., Wright, C. L., Kukulies, T. L., & Molina, A. B. (2014). Ground cover management alters development of Fusarium wilt symptoms in Ducasse bananas. Australasian Plant Pathology, 43(4), 465-476. https://doi.org/10.1007/s13313-014-0296-5
Pérez-Vicente, L., Dita, M. A., & Martínez-de la Parte, E. (2014). Prevention and diagnostic of Fusarium wilt (Panama disease) of banana caused by Fusarium oxysporum f. sp. cubense. Tropical race 4 (TR4). FAO Rome, Italy (Edn), (pp.74).
Purwati, R. D., & Harran, S. (2007). In vitro selection of abaca for resistance to Fusarium oxysporum f. sp. cubense. HAYATI Journal of Biosciences, 14(2), 65-70. https://doi.org/10.4308/hjb.14.2.65
Ravichandra, N. G. (2014). Horticultural nematology. New Dehli: Springer India. https://doi.org/10.1007/978-81-322-1841-8
Ribeiro, R. C. F., Campos, V., Xavier, A., De Souza L. Rocha, Ribeiro, H. B., Aguiar F. M., Souza R. M., Mizobutsi E. H., & Dias-Arieira, C. R. (2012). Control of Meloidogyne javanica and Panama disease with rhizobacteria. Nematropica, 42(2), 218-226.
Rocha, L. D. S., Santana, R. F. D., Soares, A. C. F., & Haddad, F. (2018). Reaction of banana cultivars to the Meloidogyne javanica × Fusarium oxysporum f. sp. cubense complex. Revista Caatinga, 31, 572-583. https://doi.org/10.1590/1983-21252018v31n305rc
Sarah, J. L. (2000). Cultural practices as a way of nematode control of banana.
Sasser, J. N. (1980). Root-knot nematodes: a global menace to crop production. Plant disease, 64(1), 36-41.
https://doi.org/10.1094/PD-64-36
Sharrock, S., & Frison, E. A. (1999). # Musa# production around the world-trends, varieties and regional importance.
Sikora, R., Coyne, D., & Quénéhervé, P. (2018). Nematode parasites of bananas and plantains. In Plant parasitic nematodes in subtropical and tropical agriculture (pp. 617-657). Wallingford UK: CAB International. https://doi.org/10.1079/9781786391247.0617
Singh, Sunil S. K., Barry, CondeC., & Mike, Hodda H. (2012). Root-knot nematode (Meloidogyne incognita) on bitter melon (Momordica charantia) near Darwin, Australia. Australasian Plant Disease Notes 7, 75-78. https://doi.org/10.1007/s13314-012-0052-z
Speijer, P. R., Gold, C. S., Karamura, E. B., Goossens, B., Elsen, A., & De Waele, D. (2000). Rate of nematode infestation of clean banana planting material (Musa spp. AAA) in Uganda. Acta Horticulturae, 461-470. https://doi.org/10.17660/ActaHortic.2000.540.52
Tariq-Khan, M., Munir, A., Mukhtar, T., Hallmann, J., & Heuer, H. (2017). Distribution of root-knot nematode species and their virulence on vegetables in northern temperate agro-ecosystems of the Pakistani-administered territories of Azad Jammu and Kashmir. Journal of Plant Diseases and Protection, 124, 201-212. https://doi.org/10.1007/s41348-016-0045-9
Taylor, A. L., & Sasser, J. N. (1978). Biology, identification and control of root-knot nematodes. North Carolina State University Graphics, (pp111).
Taylor, D. P., & Netscher, C. (1974). An improved technique for preparing perineal patterns of Meloidogyne spp. Nematologica, 20(2), 268-269.