THÀNH PHẦN HÓA HỌC, HOẠT TÍNH CHỐNG OXY HOÁ CỦA HỖN HỢP POLYSACCHARIDE LY TRÍCH TỪ RONG MƠ SARGASSUM MICROCYSTUM

Huỳnh Trường Giang * , Vũ Ngọc Út , Trương Quốc Phú và Dương Thị Hoàng Oanh

* Tác giả liên hệ (htgiang@ctu.edu.vn)

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Ngày nhận bài: 22-10-2012
Ngày duyệt đăng: 22-03-2013
Ngày xuất bản: 01-05-2013
Title: Chemical composition, antioxidant activities of polysacharide extracts from brown seaweed Sargassum microcystum
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Giang, H. T., Út, V. N., Phú, T. Q., & Oanh, D. T. H. (2013). THÀNH PHẦN HÓA HỌC, HOẠT TÍNH CHỐNG OXY HOÁ CỦA HỖN HỢP POLYSACCHARIDE LY TRÍCH TỪ RONG MƠ SARGASSUM MICROCYSTUM. Tạp chí Khoa học Đại học Cần Thơ, (25), 183-191. Truy vấn từ https://ctujsvn.ctu.edu.vn/index.php/ctujsvn/article/view/41
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Số. 25 (2013)
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Abstract

The aim of this study is to evaluate the chemical composition and antioxidant activities of polysaccharides extracted from brown seaweed Sargassum microcystum. Polysaccharides were extracted by three different extraction solvents: hot-water (100 oC), 0.1N HCl, and 90% aqueous ethanol. The results showed that among three extraction solvents, polysaccharide was extracted by 0.1N HCl exhibited higher yield of 40.2 ± 1.8% followed by hot-water (25.0 ± 1.3%) and 90% aqueous ethanol solvent (10.9 ± 0.4%). Crude protein obtained 9.3, 7.7, and 5.6% for treatments of hot-water, 0.1N HCl, and 90% aqueous ethanol, respectively. Total phlorotannins accounted for about 2.7, 6.5, and 2.1 mg/g of the hot-water, 0.1N HCl, and 90% aqueous ethanol treatments, respectively. The DPPHã free radicals scavenging activity, ferrous ion chelating activity, and Fe+3 reducing power of extracts from S. microcystum were increasing with increase of concentration. Judging from these results, it is therefore concluded that the polysaccharide extracts of brown seaweed S. microcystum possessed the good antioxidant activities and could be use in aquaculture.
Keywords: Antioxidant activity, polysaccharide, Sargassum microcystum

Tóm tắt

Nghiên cứu được thực hiện nhằm đánh giá thành phần hóa học và hoạt tính chống oxy hóa của hỗn hợp polysaccharide ly trích từ rong mơ S. microcystum. Polysaccharides được trích xuất bởi ba dung môi khai thác khác nhau: nước 100 oC, HCl 0,1N và Ethanol 90%. Kết quả cho thấy khi ly trích bằng dung môi HCl 0,1N thu được hàm lượng polysaccharide cao nhất (40,2 ± 1,8%) kế đến là dung môi nước 100 oC (25,0 ± 1,3%) và Ethanol 90% (10,9 ± 0,4%). Hàm lượng protein ở các nghiệm thức tương đối thấp, đạt giá trị 9,3; 7,7 và 5,6% đối với nghiệm thức nước 100 oC, HCl 0,1 và Ethanol 90% tương ứng. Hàm lượng phlorotannin cao nhất ở nghiệm thức HCl 0,1N (6,5 mg/g) kế đến là nghiệm thức nước 100 oC và Ethanol 90%. Hoạt tính khử gốc oxy hóa DPPHã, hoạt tính tạo phức với Fe+2 và hoạt tính khử Fe+3 gia tăng tỉ lệ thuận với sự gia tăng hàm lượng của polysaccharide. Điều này cho thấy polysaccharide ly trích từ rong mơ S. microsystum có thể sử dụng như một hợp chất giàu hoạt tính chống oxy hóa và có thể nghiên cứu ứng dụng trong nuôi trồng thủy sản để tăng cường miễn dịch của tôm cá nuôi.
Từ khóa: Hoạt tính chống oxy hóa, polysaccharide, Sargassum microcystum

Article Details

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